LOCUS       sequence-479420-         900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses multifunctional construct enabling the YES-gated release 
            of mCerulean from materials in response to TEV treatment. Construct 
            contains a SpyTag for material tethering..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gharios R, Ross ML, Li A, Kottantharayil SP, Hoye J, DeForest CA
  TITLE     Boolean logic-gated protein presentation through autonomously 
            compiled molecular topology.
  JOURNAL   Nat Chem Biol. 2025 Oct 9. doi: 10.1038/s41589-025-02037-5.
  PUBMED    41068299
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Oct 9. doi: 10.1038/s41589-025-02037-5."
COMMENT     Sequence Label: mCerulean-T-SpyTag
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             757..777
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             805..852
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
     CDS             880..897
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggatctt cgatggtctc caaaggcgaa gaactgttca cgggtgtggt cccgattctg
       61 gtggaattgg atggtgacgt aaatggccat aagttttcgg tgagcggcga aggcgaaggc
      121 gatgcgacct atggcaagtt aaccctgaaa ttcatctgta ccacgggaaa actaccggtg
      181 ccgtggccaa cgctggtgac caccctgacg tggggcgtac agtgctttgc tcgttatccg
      241 gatcacatga aacagcacga cttctttaaa agcgctatgc cggagggcta cgtgcaggaa
      301 cggacaatct tttttaaaga cgatgggaat tataaaacgc gtgccgaagt taaatttgag
      361 ggtgatactc tggttaatcg tatcgaactg aaagggatcg atttcaaaga ggatgggaac
      421 atactcggtc ataaattgga gtataacgcg atttcggata acgtttatat tacagcagac
      481 aaacagaaga acggcattaa ggccaacttt aaaatccgcc ataacattga agatggaagc
      541 gtgcagctcg cggatcacta ccaacaaaat acccccatcg gcgacggtcc tgtcctgctt
      601 cctgataatc actatttaag tactcaaagt aaactttcaa aggacccaaa cgaaaaacgc
      661 gaccacatgg tgttgctgga gttcgtcacc gccgcgggca ttactttagg tatggatgaa
      721 ctgtacaaag gtggatcagg tggttccggg ggcagcgaaa atctgtactt tcagtcaggc
      781 ggctccgggg ggtctggagg cagccgcggt gtaccgcata ttgttatggt tgatgcatac
      841 aagcgatata aaggtggcag tggtgggtct ggtggtagcc atcatcatca ccatcattaa
//
