LOCUS       sequence-47946-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   White MD, Milne RV, Nolan MF
  TITLE     A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or 
            Down-Regulate Neuronal Gene Expression in vivo.
  JOURNAL   Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.
  PUBMED    21772812
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2011"; volume: "4"; pages: "8. Epub 2011 Jul 4"
COMMENT     Sequence Label: pENTR-L3-oPRE-L2
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    111..135
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(719..814)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(831..848)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(831..847)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(840..862)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttggggaatt cgcggccgcc ctgcagctgg atggcaaata atgattttat tttgactgat
       61 agtgacctgt ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt
      121 acaagaaagc tgggtacgac aacaccacgg aattatcagt gcccagcaac ctagcccctg
      181 tccagcagcg ggcaaggcag gcggcgatga gttctgccgt ggcgatcggg agggggaaag
      241 cgaaagtccc agaaaggagt tgacaggtgg tggcaatgcc ccagccagtg ggggttgcgt
      301 cagcaaacac agagcacacc acgccacgtt gacggacaac gggccacaac tcctctaaaa
      361 gagacagcaa ccaggattta tacaaggagg agaaaacgaa agccgtacgg gaagcaatag
      421 ctagatacag aggctataaa gcagcatatc cacacagcgt aaaaggagca acatagttaa
      481 gaatatcagt caatctttca caaattttgt aatccagagg ttgattaaca ggaacagagc
      541 gtaaataacg ggaaagtttc ttaacatgtt tgtcttgtgg caatacacct gaactagtaa
      601 ttacatatcc ctaaaaatgt aaatgattgc cccaccattt tgttttatta acatttaaat
      661 gtatacccaa atcaagaaaa acagaacaaa tatgggtata aatggcggta agatgctcca
      721 actttattat acaaagttgg cattataaaa aagcattgct catcaatttg ttgcaacgaa
      781 caggtcacta tcagtcaaaa taaaatcatt atttggggcc cgagcttaag actggccgtc
      841 gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag agtagggaac
      901 tgccaggcat caataaacaa agtcatcgaa
//
