LOCUS       sequence-479481-        9523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expressing scramble of shRNA targeting Miro1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Rodriguez Salazar MP, Kolanukuduru S, Ramirez V, Lyu B, Manigault G,
            Sejourne G, Sesaki H, Yu G, Eroglu C
  TITLE     Mitochondrial fission controls astrocyte morphogenesis and 
            organization in the cortex.
  JOURNAL   J Cell Biol. 2025 Oct 6;224(10):e202410130. doi: 
            10.1083/jcb.202410130. Epub 2025 Sep 3.
  PUBMED    40899994
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2025-10-6"; volume: "224(10):e202410130. doi"; pages:
            " 10.1083/jcb.202410130. Epub 2025 Sep 3"
COMMENT     Sequence Label: pLKO.1-scrambled-GFP
FEATURES             Location/Qualifiers
     source          1..9523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    101..122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(137..142,143..160,161..167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    175..191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     180..202
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     199..215
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     199..215
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     234..254
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        236..254
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        282..508
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             509..689
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    736..861
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1354..1587
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1772..1816
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2114..2362
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2114..2134
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2285..2304
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2459..2576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2943..3246
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3599..4615
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4539..4561
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4623..4642
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4692..4701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4698..4700,4701..4703,4704..5417)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4698..4700,4701..4703,4704..5414)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4743..4764)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5004..5023)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5351..5372
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5437..5470)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5526..6114
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5579..5599)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5997..6008)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6117..6133)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6118..6134)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6323..6556
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6628..6749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6665..6684)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6719..6738
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6789..6924
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6944..6963)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6945..6963)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6973..6990)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6973..6989)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6982..7004)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7131..7586
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7218..7237)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7428..7449
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7612..7716
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7717..7785,7786..8577)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7935..7954)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8748..9336
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9237..9256
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9490..9507
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
      241 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
      301 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
      361 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
      421 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
      481 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
      541 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      601 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      661 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
      721 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
      781 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
      841 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
      901 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
      961 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1021 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1081 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1141 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1201 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1261 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1321 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1381 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1441 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1501 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1561 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1621 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     1681 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     1741 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     1801 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     1861 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     1921 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     1981 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2041 atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggc
     2101 cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     2161 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
     2221 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     2281 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     2341 cttgtggaaa ggacgaaaca ccgggttcta agttccgtgt tcaggctcga gcctgaacac
     2401 ggaacttaga actttttgaa ttctcgacct cgagacaaat ggcagtattc atccacaatt
     2461 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2521 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2581 tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc
     2641 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     2701 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     2761 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     2821 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     2881 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2941 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     3001 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     3061 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     3121 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     3181 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     3241 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     3301 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     3361 gggggggggc gcgccccggg gggggggggg gggggggggg gggggggggg gggggggggg
     3421 gggggggggg ggggggccag gggggggggg ggggggggga aggttttttt tttggggggg
     3481 gggggggagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     3541 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     3601 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
     3661 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     3721 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
     3781 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
     3841 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
     3901 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     3961 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     4021 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     4081 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     4141 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     4201 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     4261 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     4321 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     4381 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     4441 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     4501 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     4561 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     4621 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg cggccgctag
     4681 cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4741 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     4801 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4861 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     4921 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4981 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     5041 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     5101 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     5161 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     5221 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     5281 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     5341 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     5401 acgagctgta caagtaggaa ttcgtcgagg gacctaataa cttcgtatag catacattat
     5461 acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta
     5521 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5581 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5641 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5701 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5761 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5821 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5881 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5941 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6001 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6061 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     6121 accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     6181 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     6241 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6301 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     6361 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6421 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6481 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6541 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6601 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     6661 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6721 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     6781 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6841 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6901 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     6961 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7021 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     7081 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     7141 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7201 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7261 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7321 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7441 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7501 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     7561 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     7621 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7681 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7741 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7801 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7861 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7921 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7981 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8041 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8101 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8161 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8221 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8281 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8341 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8401 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8461 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8521 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8581 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8641 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8881 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9121 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9361 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9421 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9481 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcc
//
