LOCUS       sequence-479488-        6386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' AAVLINK plasmid for POGZ expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Zhonghua Lu Lab
  TITLE     AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery 
            in Gene Therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-5' POGZ
FEATURES             Location/Qualifiers
     source          1..6386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      3864..4319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3951..3970)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4161..4182
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4336..4355)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4455..4477
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4515..4533)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4601..4705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4706..4774,4775..5566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4924..4943)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5737..6325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6226..6245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctgcgg acaccgacct gttcatggaa tgtgaggagg aggagttgga
      781 gccatggcag aaaatcagtg atgtcattga ggactctgta gttgaagatt ataattcagt
      841 ggataaaact accacagttt ctgtgagcca gcagccagtc tcggctccag tgcccatcgc
      901 tgcccatgct tctgttgctg ggcacctctc tacatccacc accgttagta gcagcggggc
      961 acagaacagc gacagtacaa agaagactct tgtcacacta attgccaaca acaatgctgg
     1021 caatcctttg gtccagcaag gtggacagcc actcatcctg acccagaatc cagccccagg
     1081 tctgggcaca atggttactc aaccagtatt gaggcctgtt caggtcatgc agaatgccaa
     1141 tcatgtgact agttcccctg tggcctcaca accaatattt atcactacgc agggatttcc
     1201 tgtaaggaat gtccggcctg tacaaaatgc aatgaatcag gttgggattg tgctgaacgt
     1261 acagcaaggc caaacggtta gaccaattac actagttcca gccccaggta cccagtttgt
     1321 taagccgaca gttggagttc cacaagtgtt ctcccagatg acccctgtga ggccaggctc
     1381 cacaatgcct gtgaggccca ccaccaacac cttcaccacc gtcatcccgg ccactcttac
     1441 cattcgaagc accgtcccac agtcccagtc ccagcagacc aagtccactc ccagcacttc
     1501 taccactccc actgccacac agccaacctc actggggcaa ctagctgttc agtctccagg
     1561 ccagtcaaac cagaccacga atcccaagct agtgagcatt gccagctttg tcactgtgaa
     1621 gcgacctggt gttacaggcg aaaatagcaa tgaagtggcc aaattggtga atacccttaa
     1681 caccatccct tccctgggcc agagtcctgg gccagtggtg gtgtccaaca acagctctgc
     1741 tcatggctct caaagaacca gcggacctga gtcttcaatg aaagtgacct cttccatccc
     1801 agtatttgac ctccaggatg gtggacggaa aatatgtcca cgatgtaatg ctcaatttcg
     1861 tgttactgaa gctttgagag gtcacatgtg ttactgttgc ccagaaatgg ttgaatacca
     1921 gaagaaagga aagtccctgg attcagaacc cagtgtccca tcagcagcaa agcccccatc
     1981 ccctgagaaa acagctcctg ttgcttccac accctcttct acacctattc ctgctctgtc
     2041 accgcctacc aaagtaccag aaccaaatga gaacgtgggc gatgccgtcc agaccaaact
     2101 cattatgctt gtagatgact tctactatgg acgggatggt ggcaaagtag cccagctcac
     2161 aaatttccct aaggtcgcca catctttccg atgcccacat tgtaccaaaa ggctaaaaaa
     2221 caatattcga ttcatgaacc atatgaaaca ccacgtagaa ctcgatcagc agaacggtga
     2281 ggtagatggt cacactatct gccagcactg ttaccgccag ttttccactc ccttccagct
     2341 tcagtgccac ttggaaaatg ttcatagtcc ctatgaatct actaccaagt gcaagatctg
     2401 tgaatgggcg tttgaaagtg agccactatt tctccagcat atgaaggata ctcataagcc
     2461 tggagagatg ccttatgttt gccaggtgtg tcaatatcgc tcctcactct actctgaggt
     2521 agatgtccat tttcggatga tccatgagga tacccggcat ctgctctgcc cttattgcct
     2581 gaaggtcttc aaaaatggca atgcattcca acagcattac atgaggcacc agaagagaaa
     2641 tgtttatcac tgcaacaaat gccagctgca gtttctcttt gccaaggaca aaattgaaca
     2701 caagcttcaa caccataaaa ccttccgtaa acccaagcag ctggagggct tgaaaccagg
     2761 caccaaggtg acaatccggg cttcccgagg gcagccacga actgttcctg tatcctctaa
     2821 tgatacacct cccagcgcct tgcaggaggc agcaccgctg acctcctcaa tggaccctct
     2881 gcctgtcttc ctttatcccc ctgtccagcg cagcatccag aagagagctg ttaggaaaat
     2941 gagtgtcatg ggccggcaga catgcctgga gtgcagcttc gagatcccag acttccctaa
     3001 tcatttccct acttacgtac actgctctct gtgtcgctat agcacctgct gttctcgagc
     3061 ttatgccaac cacatgatca acaatcatgt tccacggaag agccccaagt atttggcttt
     3121 gtttaaaaat tctgtgagtg gaatcaagct ggcctgcact tcatgtacct ttgttacctc
     3181 tgtgggcgat gctatggcca agcatttggt attcaacccc tctcacagat ccagcagcat
     3241 cctgccacgg ggactcactt ggatagctca ctcaaggcat ggccagactc gtgaccgagt
     3301 gcatgaccgg aacgtgaaga atatgtaccc tcctccttcc ttccccacta acaaagctgc
     3361 cactgtgaaa tctgcggggg ccaccccagc tgagcctgaa gagctactaa ctcccttagc
     3421 cccagcactc ccatcaccag cctcaactgc aaccccacca ccaaccccca ctcacccgca
     3481 ggctttagcc cttccaccgc tggctacaga gggagccgaa tgtctgaatg ttgatgatca
     3541 ggatgaaggt aagtatcaag gttacaagac aggtttaagg agaccaataa gctattattc
     3601 gtataggata ctttatacga agttattaag ttatggagac cggtgtggca cgtgcggacc
     3661 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     3721 ctcactgagg ccgggcgacc aaaggtcgcc cggcctcagt gagcgagcga gcgcgcagct
     3781 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3841 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3901 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
     3961 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4021 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     4081 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4141 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
     4201 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
     4261 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     4321 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     4381 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4441 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4501 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4561 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4621 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4681 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4741 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4801 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4861 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4921 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4981 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5041 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5101 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5161 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5221 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5281 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5341 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5401 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5461 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5521 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5581 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5641 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5881 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6121 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6301 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6361 cttttgctgg ccttttgctc acatgt
//
