LOCUS       sequence-47952-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Zou J, Mali P, Huang X, Dowey SN, Cheng L
  TITLE     Site-specific gene correction of a point mutation in human iPS cells
            derived from an adult patient with sickle cell disease.
  JOURNAL   Blood. 2011 Oct 27;118(17):4599-608. Epub 2011 Aug 31.
  PUBMED    21881051
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood."; 
            date: "2011-10-27"; volume: "118(17)"; pages: "4599-608. Epub 2011 
            Aug 31"
COMMENT     Sequence Label: BD2
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    62..286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     275..292
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(371..392)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     720..739
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctactatagg gcgaattggg ccctctagat gcatgctcga aattccgcgg gcccgctcat
       61 tccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
      121 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
      181 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
      241 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggcgttgacg
      301 cggccgcttt acttgtacgg ccgctttact tgtacagctc gtccatgccg agagtgatcc
      361 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      421 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
      481 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
      541 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
      601 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      661 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      721 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
      781 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
      841 cgccgtaggt caaggtggtc acgagggtgg gccaagggc
//
