LOCUS       sequence-479767-       17226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17226)
  AUTHORS   Sung YC, Li Y, Bernasconi Z, Baik S, Asuke S, Keller B, Fahima T, 
            Coaker G
  TITLE     Wheat tandem kinase RWT4 directly binds a fungal effector to 
            activate defense.
  JOURNAL   Nat Genet. 2025 Apr 14. doi: 10.1038/s41588-025-02162-w.
  PUBMED    40229601
REFERENCE   2  (bases 1 to 17226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 Apr 14. doi: 10.1038/s41588-025-02162-w."
COMMENT     Sequence Label: pTA7001-RWT4Cs
FEATURES             Location/Qualifiers
     source          1..17226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  1335..2102
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2294..3088
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3387..4535
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6131..6155
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(6402..6423)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6614..6633
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6758..6780
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6772..6789
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6773..6789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6999..7045
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    7077..7101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9885..9908
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(9915..9939)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9947..10012
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      complement(10538..10790)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(11905..12088)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(11941..12120)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(13412..13645)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        complement(13907..14252)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    14767..14791
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             15256..15906
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(16094..16111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(16345..16364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            16587..16696
                     /note="incP origin of transfer"
     CDS             16729..17100
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 gcgcctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agcgctggca
      721 gtccttgcca ttgccgggat cggggcagta acgggatggg cgatcagccc gagcgcgacg
      781 cccggaagca ttgacgtgcc gcaggtgctg gcatcgacat tcagcgacca ggtgccgggc
      841 agtgagggcg gcggcctggg tggcggcctg cccttcactt cggccgtcgg ggcattcacg
      901 gacttcatgg cggggccggc aatttttacc ttgggcattc ttggcatagt ggtcgcgggt
      961 gccgtgctcg tgttcggggg tgcgataaac ccagcgaacc atttgaggtg ataggtaaga
     1021 ttataccgag gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat
     1081 atttaaaaag ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat
     1141 atattgacaa tactgataag ataatatatc ttttatatag aagatatcgc cgtatgtaag
     1201 gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga atgggcaaag
     1261 cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat agcttgtaaa
     1321 ttctatcata attgggtaat gactccaact tattgatagt gttttatgtt cagataatgc
     1381 ccgatgactt tgtcatgcag ctccaccgat tttgagaacg acagcgactt ccgtcccagc
     1441 cgtgccaggt gctgcctcag attcaggtta tgccgctcaa ttcgctgcgt atatcgcttg
     1501 ctgattacgt gcagctttcc cttcaggcgg gattcataca gcggccagcc atccgtcatc
     1561 catatcacca cgtcaaaggg tgacagcagg ctcataagac gccccagcgt cgccatagtg
     1621 cgttcaccga atacgtgcgc aacaaccgtc ttccggagac tgtcatacgc gtaaaacagc
     1681 cagcgctggc gcgatttagc cccgacatag ccccactgtt cgtccatttc cgcgcagacg
     1741 atgacgtcac tgcccggctg tatgcgcgag gttaccgact gcggcctgag ttttttaagt
     1801 gacgtaaaat cgtgttgagg ccaacgccca taatgcgggc tgttgcccgg catccaacgc
     1861 cattcatggc catatcaatg attttctggt gcgtaccggg ttgagaagcg gtgtaagtga
     1921 actgcagttg ccatgtttta cggcagtgag agcagagata gcgctgatgt ccggcggtgc
     1981 ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg atagaaacag
     2041 aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca
     2101 cccataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     2161 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     2221 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     2281 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     2341 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     2401 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     2461 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     2521 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     2581 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     2641 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     2701 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     2761 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     2821 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     2881 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     2941 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     3001 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     3061 ttatatttta ctggatgaat tgttttagta cctagatgtg gcgcaacgat gccggcgaca
     3121 agcaggagcg caccgacttc ttccgcatca agtgttttgg ctctcaggcc gaggcccacg
     3181 gcaagtattt gggcaagggg tcgctggtat tcgtgcaggg caagattcgg aataccaagt
     3241 acgagaagga cggccagacg gtctacggga ccgacttcat tgccgataag gtggattatc
     3301 tggacaccaa ggcaccaggc gggtcaaatc aggaataagg gcacattgcc ccggcgtgag
     3361 tcggggcaat cccgcaagga gggtgaatga atcggacgtt tgaccggaag gcatacaggc
     3421 aagaactgat cgacgcgggg ttttccgccg aggatgccga aaccatcgca agccgcaccg
     3481 tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc gatggtccag caagctacgg
     3541 ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg
     3601 ccgtggagcg ttcgcgtcgt ctcgaacagg aggcggcagg tttggcgaag tcgatgacca
     3661 tcgacacgcg aggaactatg acgaccaaga agcgaaaaac cgccggcgag gacctggcaa
     3721 aacaggtcag cgaggccaag caggccgcgt tgctgaaaca cacgaagcag cagatcaagg
     3781 aaatgcagct ttccttgttc gatattgcgc cgtggccgga cacgatgcga gcgatgccaa
     3841 acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc
     3901 tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa gatcacctac accggcgtcg
     3961 agctgcgggc cgacgatgac gaactggtgt ggcagcaggt gttggagtac gcgaagcgca
     4021 cccctatcgg cgagccgatc accttcacgt tctacgagct ttgccaggac ctgggctggt
     4081 cgatcaatgg ccggtattac acgaaggccg aggaatgcct gtcgcgccta caggcgacgg
     4141 cgatgggctt cacgtccgac cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct
     4201 tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa
     4261 tcgtcgtgct gtttgctggc gaccactaca cgaaattcat atgggagaag taccgcaagc
     4321 tgtcgccgac ggcccgacgg atgttcgact atttcagctc gcaccgggag ccgtacccgc
     4381 tcaagctgga aaccttccgc ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc
     4441 gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct
     4501 gggtcaatga tgacctggtg cattgcaaac gctagggcct tgtggggtca gttccggctg
     4561 ggggttcagc agccagcgct ttactggcat ttcaggaaca agcgggcact gctcgacgca
     4621 cttgcttcgc tcagtatcgc tcgggacgca cggcgcgctc tacgaactgc cgataaacag
     4681 aggattaaaa ttgacaattg tgattaaggc tcagattcga cggcttggag cggccgacgt
     4741 gcaggatttc cgcgagatcc gattgtcggc cctgaagaaa gctccagaga tgttcgggtc
     4801 cgtttacgag cacgaggaga aaaagcccat ggaggcgttc gctgaacggt tgcgagatgc
     4861 cgtggcattc ggcgcctaca tcgacggcga gatcattggg ctgtcggtct tcaaacagga
     4921 ggacggcccc aaggacgctc acaaggcgca tctgtccggc gttttcgtgg agcccgaaca
     4981 gcgaggccga ggggtcgccg gtatgctgct gcgggcgttg ccggcgggtt tattgctcgt
     5041 gatgatcgtc cgacagattc caacgggaat ctggtggatg cgcatcttca tcctcggcgc
     5101 acttaatatt tcgctattct ggagcttgtt gtttatttcg gtctaccgcc tgccgggcgg
     5161 ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc gtgttcatct ctgccgctct
     5221 gctaggtagc ccgatacgat tgatggcggt cctgggggct atttgcggaa ctgcgggcgt
     5281 ggcgctgttg gtgttgacac caaacgcagc gctagatcct gtcggcgtcg cagcgggcct
     5341 ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc cgcaagtggc aacctcccgt
     5401 gcctctgctc acctttaccg cctggcaact ggcggccgga ggacttctgc tcgttccagt
     5461 agctttagtg tttgatccgc caatcccgat gcctacagga accaatgttc tcggcctggc
     5521 gtggctcggc ctgatcggag cgggtttaac ctacttcctt tggttccggg ggatctcgcg
     5581 actcgaacct acagttgttt ccttactggg ctttctcagc cccagatctg gggtcgatca
     5641 gccggggatg catcaggccg acagtcggaa cttcgggtcc ccgacctgta ccattcggtg
     5701 agcaatggat aggggagttg atatcgtcaa cgttcacttc taaagaaata gcgccactca
     5761 gcttcctcag cggctttatc cagcgatttc ctattatgtc ggcatagttc tcaagatcga
     5821 cagcctgtca cggttaagcg agaaatgaat aagaaggctg ataattcgga tctctgcgag
     5881 ggagatgata tttgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
     5941 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
     6001 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
     6061 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
     6121 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
     6181 acacattgcg gacgttttta atgtactggg gtggtttttc ttttcaccag tgagacgggc
     6241 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     6301 gtttgcccca gcaggcgaaa atcctgtttg atggtggttc cgaaatcggc aaaatccctt
     6361 ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6421 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6481 gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6541 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6601 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc cattcaggct gcgcaactgt
     6661 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6721 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6781 acggccagtg aattcctcga agcttgcatg ccggtcgact ctagaggatc cgggtgacag
     6841 ccctccgacg ggtgacagcc ctccgacggg tgacagccct ccgaattcta gaggatccgg
     6901 gtgacagccc tccgacgggt gacagccctc cgacgggtga cagccctccg aattcgagct
     6961 cggtacccgg ggatctgtcg acctcgatcg agatcttcgc aagacccttc ctctatataa
     7021 ggaagttcat ttcatttgga gaggacacgc tgaagctagt cgactctagc ctcgagacaa
     7081 gtttgtacaa aaaagcaggc tccaccatga tgggtggcta tgaatttcaa cgagcagaat
     7141 tggacgctct ggagggagtc gtcagagacc caactgcaga accaatgtcc cttacattac
     7201 cccttttacg tcacataacg aatgactttt ccccagagtt tgagatcagc aaggatgatt
     7261 ccgccgtggt ttatctcggc gttctcccaa gtggtttcag agtcgcagtg aagaagctcc
     7321 ataaccgaca ttggctggaa gatgaggatg cctttattaa tgaggtaagt atcgctatga
     7381 aggccgctaa aaagaacacg gtccgtgtta ttggctattg tcagcatacg caagagcaaa
     7441 tcgcagaata cgaaggaaaa caagtgttcg ccgagcttac ggagcgtttg atctgcaccg
     7501 aatacgtccc caacggaccg ctctctggcc acattgaggg gaaaatatgt gcacaaatgg
     7561 acggatatga gtttcaaagg gcagagttgg acgcactgga aagagtcgta cgagacactt
     7621 cagctgaacc gatgtccttg actcttccgt tgctcagaca tattacgaat gacttttccg
     7681 acgaaagccg aataggcagg ggaggctttg ccgtggtata cctgggagtt ttacctagcg
     7741 gtttgaggat tgctgttaag aggcttagta atattgccta catgaatgaa tcagcttttc
     7801 aaaacgaggt tttcattacg atgaaagcca cccataaaaa taccgtccga ttcatgggct
     7861 attgctcaca aatacaggga aaattaatag agcacaatgg gcagcatgtc tttgctcagt
     7921 tagaggagag gctgatttgt gtcgaatacg ctccaaaagg cactttagac gcacatatag
     7981 gggactacgg ggagctcgac tggaaccaac gataccagat acttaagggg atatgtcaag
     8041 gcctccacca cctccacgat gagatgcatg tctttcacgg tgatatcaag cctgccaata
     8101 tcttgattgg tgacaatctc gtccccaaaa tctacgattt tggactttca caaatgtttg
     8161 aagaagaaga gacggagcga atcgtaaaaa atatggctgg tacgttgtgc tacctcgcac
     8221 cggaggttct taacacccat atgatgtctt ttaaggctga gatatattct ttaggtgtgg
     8281 tgattgagga attactgacg ggtaagaagg gctggatcga tgaagacgtt agaaagcaac
     8341 tcaagggcct cagaaagaca ttagtgaagg aaggtgcttt ttcaagttgg gagaacaaat
     8401 accatcaggt aaggacctgc atggaaattg gtcaggactg tatcgatccc aatccgcata
     8461 agaggcccac cctgttcgaa attattcagc gtcttaatga agctgaggac atgaattaca
     8521 gtgccgcaag cttgtggcag gttcagtctg gtgacgaaga atcagacttg tctgacacgg
     8581 aagcattaga aaccgaaaca actagcgagt tcctcccgtc agacgaggag ccggcaagtg
     8641 ttggtaagac tggggagact tctactcagg aacccgataa gccagacttg gtttcaaagc
     8701 tgccagcaag cgtcgatctg tccgatttaa aagtgctcga aaaaataaca gatgacttca
     8761 gccatgagag aatcgttggc aaggacggta cattcaaggg ttgccacaaa gcattcgtgt
     8821 ataagggcga cattccgcga cgtgagatga tcgccgtcaa aagactcatc ggtgttgaaa
     8881 tacccgtcga aaagttcaag agggaggctg aacagttcat tcgtttagat cataagaaca
     8941 ttgttaaagt cgcatcctat tgccacgacc aatccactgg tcacaggctc gtgcagttta
     9001 aaggaaaacc attgccccag ttatttaacg gtcctgaaca gttgctctgc tatgagtata
     9061 tgcataatgg gtctttgcgt gactatctta tgggtcaggg tagccgtgaa atcgattggc
     9121 agatgcgtta taaactgatc aaagggactt gcgcaggcct tgattacctt cataaggggc
     9181 gagctggttg cccgattgtc catctcaact taagcccgag caacgtttta ttggatcata
     9241 actacatacc taggattacc ggcttcgatt tctctaagtt gattggagaa aaaaatacga
     9301 agagtgtagt tctcaaactc aacggaccaa ttgcctatct tcctccggat tttttccaca
     9361 gtaaaggcac cgacttgaag tacctggcaa ccgtcgacat ttactcactt gggttgatga
     9421 tcctggaaat tgctacgcaa caagaaatta agggaattca cggagtgctg atcaagagta
     9481 tcgaagagaa ctggagggag gagagtcaga taacccgatt gtatacctcc ttgggagctg
     9541 atgaattacg tcaagtgaaa atgtgcattg atatcggcct ggactgcgtt aagtcaaacc
     9601 ctgaaaaaag gcctaccgca ggggctataa tgctctggtt ggataaggaa tctaaacccg
     9661 taccagtatc aagggctggg gccggagtct taccacgacc ccctgtacct actaacataa
     9721 accacgctgg taggatacag ggaataccaa ttgaaaaaag ccaggtctta atccctagct
     9781 tcgcatgtaa cgaatattcc tcagttaaga ttcaaaacct gcaccttttg ctcttcatgt
     9841 tcatacaagt tgaccaaggt agcgactaca aggaccacga cggtgactac aaggacgacg
     9901 acgacaagat ctagacccag ctttcttgta caaagtggtg ctcgacgatt ataaggatca
     9961 tgatggagat tataaggatc atgatattga ttataaggat gatgatgata agtaaactag
    10021 tcgatccagg cctcccagct ttcgtccgta tcatcggttt cgacaacgtt cgtcaagttc
    10081 aatgcatcag tttcattgcc cacacaccag aatcctacta agtttgagta ttatggcatt
    10141 ggaaaagctg ttttcttcta tcatttgttc tgcttgtaat ttactgtgtt ctttcagttt
    10201 ttgttttcgg acatcaaaat gcaaatggat ggataagagt taataaatga tatggtcctt
    10261 ttgttcattc tcaaattatt attatctgtt gtttttactt taatgggttg aatttaagta
    10321 agaaaggaac taacagtgtg atattaaggt gcaatgttag acatataaaa cagtctttca
    10381 cctctctttg gttatgtctt gaattggttt gtttcttcac ttatctgtgt aatcaagttt
    10441 actatgagtc tatgatcaag taattatgca atcaagttaa gtacagtata ggctttttgt
    10501 gtcgaggggg taccgagtcg aggggggatc aattcccgat ctagtaacat agatgacacc
    10561 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
    10621 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
    10681 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
    10741 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc
    10801 gggggatcag cttgtgatca tgttacatca cctccaagat cccggacgag tgctggggcg
    10861 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
    10921 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
    10981 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
    11041 aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct
    11101 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
    11161 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
    11221 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
    11281 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
    11341 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
    11401 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
    11461 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag
    11521 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
    11581 gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg gaatcgggag
    11641 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
    11701 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
    11761 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
    11821 gacagacgtc gcggtgagtt caggcttttt catatctcat tgccccccgg gatcttgcag
    11881 gtcgagatcc tctagcggat ccggtgcaga ttatttggat tgagagtgaa tatgagactc
    11941 taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt
    12001 tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg
    12061 tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc
    12121 tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact
    12181 cccttaattc tccgctcatg atcagattgt cgtttcccgc cttcagtttc aagcttgttt
    12241 ggggatctag tgttttactc ctcatattaa cttcggtcat tagaggccac gatttgacac
    12301 atttttactc aaaacaaaat gtttgcatat ctcttataat ttcaaattca acacacaaca
    12361 aataagagaa aaaacaaata atattaattt gagaatgaac aaaaggacca tatcattcat
    12421 taactcttct ccatccattt ccatttcaca gttcgatagc gaaaaccgaa taaaaaacac
    12481 agtaaattac aagcacaaca aatggtacaa gaaaaacagt tttcccaatg ccataatact
    12541 cgaacaagct agcttactca gttaggtcga gtcatttttg atgaaacaga agctttttga
    12601 tatttccatt tgaatatttt ggtatctgat tagtgatgat ttcagctaac atctctggga
    12661 attcaatact catggtctta tccaaaaatg tctggaagca gtaggtaagg agattctcaa
    12721 ccacctcatg catggagtcc agaagctttg tcagttggta aaaccgttgc cagttctgac
    12781 tggagttccc ttcccttttg acgatggctt ttcctagctc tttgatataa gtcattcgaa
    12841 tctcatcaaa taactcttgg ctcttcagac cttccttagg aactgaggag agaagcagta
    12901 aggttttcat acagagatac tcttcatagg atacctgcaa tctttgtaat tcagaggaga
    12961 caaacagcat gtgtttacat tggtcataca tgcagggtag agacattctc tgctcattaa
    13021 taatcagatc aggagcaaag cagagcaggt ttccacttga ttgtctgtat gatctccaac
    13081 ccagggcaaa tgccatgaga aacatccatg agtactgtag cagggtcatt tggtcatcca
    13141 ggtgtaagtt tctgaagcct ggtatcgcct ttgcccattt cactgctgca atcacttgac
    13201 gcccacctaa catgttgagt gtggtcataa ttctccatgc tgaatctgga acagagctat
    13261 catatcctgc atacaacacc tcgggttcaa tcacctccag cagtgacacc aaggtagggg
    13321 tgagctgtgg taatgcagca ggaactattg ttttgttagg attttccgaa gtgtcttgtg
    13381 agactcctgc agtggcttgc tgaattggat ccccaccgta ctcgtcaatt ccaagggcat
    13441 cggtaaacat ctgctcaaac tcgaagtcgg ccatatccag agcgccgtag ggggcggagt
    13501 cgtggggggt aaatcccgga cccggggaat ccccgtcccc caacatgtcc agatcgaaat
    13561 cgtctagcgc gtcggcatgc gccatcgcca cgtcctcgcc gtctaagtgg agctcgtccc
    13621 ccaggctgac atcggtcggg ggggcgctcg acctaggaaa aatcagtaga aatagctgtt
    13681 ccagtctttc tagccttgat tccacttctg tcagatgtgc cctagtcagc ggagaccttt
    13741 tggttttggg agagtagcga cactcccagt tgttcttcag acacttggcg cacttcggtt
    13801 tttctttgga gcacttgagc tttttaagtc ggcaaatatc gcatgcttgt tcgatagaag
    13861 acagtagctt cattggatct gccattgttt aaactagagt cccccgtgtt ctctccaaat
    13921 gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat
    13981 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
    14041 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
    14101 aggcatcttc aacgatggcc tttcctttat cgcaatgatg gcatttgtag gagccacctt
    14161 ccttttccac tatcttcaca ataaagtgac agatagctgg gcaatggaat ccgaggaggt
    14221 ttccggatat taccctttgt tgaaaagtct caattgccct ttggtcttct gagactgtat
    14281 ctttgatatt tttggagtag acaagtgtgt cgtgctccac catgttgacg aagattttct
    14341 tcttgtcatt gagtcgtaag agactctgta tgaactgttc gccagtcttt acggcgagtt
    14401 ctgttaggtc ctctatttga atctttgact ccatggcctt tgattcagtg ggaactacct
    14461 ttttagagac tccaatctct attacttgcc ttggtttgtg aagcaagcct tgaatcgtcc
    14521 atactggaat agtacttctg atcttgagaa atatatcttt ctctgtgttc ttgatgcagt
    14581 tagtcctgaa tcttttgact gcatctttaa ccttcttggg aaggtatttg atttcctgga
    14641 gattattgct cgggtagatc gtcttgatga gacctgctgc gtaagcctct ctaaccatct
    14701 gtgggttagc attctttctg aaattgaaaa ggctaatctg gggacctgca ggaaactatc
    14761 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
    14821 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
    14881 accacagggt tccccagatc tggcgccggc cagcgagacg agcaagattg gccgccgccc
    14941 gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca aattgcacca acgcatacag
    15001 cgccagcaga atgccatagt gggcggtgac gtcgttcgag tgaaccagat cgcgcaggag
    15061 gcccggcagc accggcataa tcaggccgat gccgacagcg tcgagcgcga cagtgctcag
    15121 aattacgatc aggggtatgt tgggtttcac gtctggcctc cggaccagcc tccgctggtc
    15181 cgattgaacg cgcggattct ttatcactga taagttggtg gacatattat gtttatcagt
    15241 gataaagtgt caagcatgac aaagttgcag ccgaatacag tgatccgtgc cgccctggac
    15301 ctgttgaacg aggtcggcgt agacggtctg acgacacgca aactggcgga acggttgggg
    15361 gttcagcagc cggcgcttta ctggcacttc aggaacaagc gggcgctgct cgacgcactg
    15421 gccgaagcca tgctggcgga gaatcatacg cattcggtgc cgagagccga cgacgactgg
    15481 cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg cgctgctcgc ctaccgcgat
    15541 ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc agatggaaac ggccgacgcg
    15601 cagcttcgct tcctctgcga ggcgggtttt tcggccgggg acgccgtcaa tgcgctgatg
    15661 acaatcagct acttcactgt tggggccgtg cttgaggagc aggccggcga cagcgatgcc
    15721 ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc cgctgttgcg ggccgcgata
    15781 gacgccttcg acgaagccgg tccggacgca gcgttcgagc agggactcgc ggtgattgtc
    15841 gatggattgg cgaaaaggag gctcgttgtc aggaacgttg aaggaccgag aaagggtgac
    15901 gattgatcag gaccgctgcc ggagcgcaac ccactcacta cagcagagcc atgtagacaa
    15961 catcccctcc ccctttccac cgcgtcagac gcccgtagca gcccgctacg ggctttttca
    16021 tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc ctctctggcg gccttctggc
    16081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    16141 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    16201 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    16261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    16321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    16381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    16441 gaagcgtggc gcttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg
    16501 gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt
    16561 ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc
    16621 gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct
    16681 ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat ggctgatgaa
    16741 accaagccaa ccaggaaggg cagcccacct atcaaggtgt actgccttcc agacgaacga
    16801 agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc
    16861 gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg cgagctggcc
    16921 cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggct caccgacgac
    16981 ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa gatcgaagag
    17041 aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc agagccatga
    17101 cttttttagc cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg tccccatgcg
    17161 ctccatcaag aagagcgact tcgcggagct ggtgaagtac atcaccgacg agcaaggcaa
    17221 gaccga
//
