LOCUS       sequence-479775-        9641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 expression cassette encoding a guide RNA targeting the Dhps 
            locus, flanked by a hammerhead (HH) ribozyme at the 5′ end and a 
            hepatitis delta virus (HDV) ribozyme at the 3′ end.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Sayson SG, Ashbaugh A, Bauer LC, Smulian AG
  TITLE     Extracellular vesicle-mediated delivery of genetic material for 
            transformation and CRISPR/Cas9-based gene editing in Pneumocystis 
            murina.
  JOURNAL   mBio. 2025 Sep 23:e0182525. doi: 10.1128/mbio.01825-25.
  PUBMED    40985725
REFERENCE   2  (bases 1 to 9641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2025 
            Sep 23:e0182525. doi: 10.1128/mbio.01825-25."
COMMENT     Sequence Label: pSS2.1-Cas9-HH-gRNADhps-HDV
FEATURES             Location/Qualifiers
     source          1..9641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..25
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7..25
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             205..4311
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             205..4308
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     ncRNA           4318..4354
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        4378..4453
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           4458..4525
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(4706..4725)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4707..4725)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4936..5524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5016..5035)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7922..8716)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8033..8052)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8643..8662
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gctagcaatt aaccctcact aaagggagac tctacgtcaa ttgcacaaat ttgagaaaag
       61 agacggatat caagaaaaat atatttttca aacgctaaaa caaataacgg gtaaagcaaa
      121 tacacagtcc ggaaataacc cctttaatac gaaaataata attaacattc tcataaactc
      181 ccgatctgac ctaaggtcaa aaaaatggat aaaaaatatt ctattggact tgatattgga
      241 acaaattctg ttggttgggc tgttattaca gatgaatata aagtaccttc aaaaaaattt
      301 aaggttcttg ggaatacaga tcgacattct ataaaaaaaa atcttatagg agctctttta
      361 tttgattcag gtgagacagc agaggcaaca agattaaaaa gaactgctcg tagaaggtat
      421 acacgaagaa aaaatcgtat ttgttattta caagagatat ttagtaatga gatggcaaaa
      481 gttgatgatt cattttttca tagattggaa gaatcttttc ttgtagaaga ggacaaaaag
      541 catgaacgtc atcctatttt tggaaatata gttgatgagg tagcttatca tgagaagtat
      601 ccaacaattt atcacttaag aaaaaaactt gtagattcaa cagataaggc tgatttaagg
      661 ttaatatatt tagctttagc acatatgata aaatttagag gacatttttt gatagaggga
      721 gatcttaatc ctgataattc tgatgttgat aaattattta tacaattggt tcaaacatat
      781 aaccagttgt ttgaggaaaa tccaattaat gcaagtggag ttgatgcaaa agcaattctt
      841 tcagcaagac tttctaaatc aaggcgatta gagaatttaa tagcacaact tccaggagag
      901 aaaaaaaatg gtttgtttgg taatttaata gctttatctc ttgggttaac tccaaatttt
      961 aaatcaaact ttgatcttgc agaagatgct aaattgcaat tgtctaaaga tacttatgat
     1021 gatgatttag ataatctttt agcacaaatt ggagatcaat atgcagactt gtttttggca
     1081 gcaaaaaatc tttcagatgc tatattactt tctgatattc ttcgtgttaa tacagagatt
     1141 acaaaggctc ctttatcagc aagtatgatt aagagatatg acgagcatca tcaagatctt
     1201 actcttttga aggctttagt tagacaacaa cttcctgaaa aatataaaga gatttttttt
     1261 gatcaaagta agaatggtta tgcaggatat attgatggtg gagcatctca agaggaattt
     1321 tataaattta taaaaccaat tcttgagaag atggatggaa ctgaagaatt attagttaaa
     1381 cttaacagag aagatttgct tagaaaacag cgtacttttg ataatggatc tataccacat
     1441 cagattcatt taggagagtt acatgctatt cttagaagac aagaggactt ttatcctttt
     1501 ttgaaggata atagagagaa gatagaaaaa atacttacat ttcgaattcc atattatgta
     1561 ggtccattag caagaggaaa ttcaagattt gcatggatga ctagaaaaag tgaagaaaca
     1621 ataactccat ggaattttga ggaggtagta gataaaggtg catcagctca atcttttata
     1681 gaaagaatga caaattttga taagaatttg cctaatgaga aggttttgcc aaaacattca
     1741 ttattatatg agtattttac agtttataat gaacttacaa aggtaaaata tgttactgaa
     1801 gggatgagaa aaccagcttt tcttagtgga gaacaaaaaa aggctattgt tgatttactt
     1861 tttaaaacta atcgaaaagt tactgtaaaa caacttaaag aggattattt taaaaaaatt
     1921 gagtgttttg attctgttga aatatcaggg gttgaagatc gttttaatgc ttcacttgga
     1981 acttatcatg atcttcttaa aattataaaa gataaggatt ttttggataa tgaagaaaat
     2041 gaagatattc ttgaagatat agttcttaca ttaactttat ttgaagatag agaaatgatt
     2101 gaggaaaggc ttaaaactta tgctcatctt tttgacgata aagttatgaa acaattaaaa
     2161 cgtcgtagat atacaggatg gggtcgactt tctagaaaat tgattaacgg tattcgtgat
     2221 aagcaatcag gaaagacaat tttagatttt ttaaaatcag atggatttgc aaatagaaat
     2281 tttatgcaat taatacatga tgattcactt acatttaaag aggatattca aaaagcacaa
     2341 gtttcaggtc aaggtgatag tcttcatgaa catattgcta atttagcagg atctccagca
     2401 attaagaaag gtattcttca aacagtaaaa gttgttgatg aacttgttaa agtaatggga
     2461 cgtcataaac ctgaaaatat tgttatagag atggcaaggg agaatcagac aacacaaaaa
     2521 ggtcagaaaa attctaggga acgtatgaag cgaattgagg aaggaattaa ggaattagga
     2581 tctcaaattc ttaaagaaca tccagtagag aatacacaac ttcaaaatga gaagctttat
     2641 ttgtattatt tacaaaacgg tagggatatg tatgttgatc aggaattaga tattaatcgt
     2701 ctttcagatt atgatgtaga tcatattgtt cctcaatctt ttttgaagga tgattcaata
     2761 gataacaaag tattaacaag atcagataaa aatcgtggta aatcagacaa cgttccatct
     2821 gaggaggttg taaagaaaat gaagaattat tggcgtcagt tacttaacgc taaattgata
     2881 acacaaagaa aatttgataa tttgacaaag gcagaaaggg gaggactttc tgagttggat
     2941 aaagcaggtt ttataaagag acaattagtt gagactcgtc aaattacaaa acatgttgct
     3001 cagattttgg atagtcgtat gaatacaaaa tatgatgaga atgataaact tattagagag
     3061 gtaaaagtta taacattaaa atctaaatta gtatctgatt ttaggaaaga ttttcaattt
     3121 tataaggttc gtgagattaa taattatcat catgcacatg atgcttatct taatgcagtt
     3181 gttggaacag ctcttattaa aaaatatcca aaattagaat ctgagtttgt ttatggagat
     3241 tataaggttt atgatgttcg taaaatgatt gcaaaatctg agcaagaaat aggaaaagca
     3301 acagcaaaat atttttttta ttctaatatt atgaattttt ttaaaacaga aataactttg
     3361 gctaacggag aaattagaaa gagaccatta attgagacta atggtgagac aggagagatt
     3421 gtttgggata aaggaagaga ttttgctaca gtaagaaagg ttttaagtat gccacaggtt
     3481 aatatagtta aaaaaactga ggttcaaaca ggaggatttt ctaaagagtc aatacttcca
     3541 aaaaggaatt cagataaatt gattgctcga aaaaaagatt gggatccaaa gaaatatggt
     3601 ggatttgatt cacctactgt tgcatattca gtattagttg tagcaaaagt tgaaaaaggt
     3661 aaatcaaaaa aacttaaatc agttaaagaa ttattaggaa taacaattat ggaacgttct
     3721 tcatttgaaa aaaatccaat agattttctt gaggctaaag gttataaaga ggttaagaaa
     3781 gatcttatta ttaaattacc taaatattca ctttttgagc ttgaaaatgg tcgaaaacgt
     3841 atgcttgcat ctgctggaga attacaaaag ggaaatgaac ttgcacttcc tagtaaatat
     3901 gtaaattttt tgtatttagc ttcacactat gaaaagttaa agggatctcc tgaagataat
     3961 gagcaaaaac agttgtttgt agagcaacat aaacattatc ttgatgaaat tattgagcag
     4021 atatctgaat tttcaaaaag ggtaattctt gcagatgcaa atcttgacaa ggttttgtca
     4081 gcttataata aacacagaga taaacctatt agagagcaag ctgaaaatat aattcatttg
     4141 tttacattaa caaatcttgg tgctcctgca gcatttaaat attttgatac tacaattgat
     4201 cgaaagagat atacttctac taaagaggtt ttggatgcaa ctcttataca tcaatctatt
     4261 acaggacttt atgaaactag aattgatctt tcacaattag gaggtgatta atcctttctg
     4321 atgagtccgt gaggacgaaa cgagtaagct cgtcaaaggt ataatatgcg aacctgggtt
     4381 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     4441 accgagtcgg tgcttttggc cggcatggtc ccagcctcct cgctggcgcc ggctgggcaa
     4501 catgcttcgg catggcgaat gggacggtga gtgacactac gacgataagg agaatgtact
     4561 ggattttgat aagcgttgtt atcttgatta tttacttcaa tttatttttt gtttttatat
     4621 ttgtttttaa tattgtattc tagtctttaa actgtttatt taaatagtat tgccttaaag
     4681 caatacgtag aatgctactc accctcccta tagtgagtcg tattaggcca caatcgtgaa
     4741 tgtgtttgcg aatttagagg tatgtatttt atcagattat tttactataa cattcataat
     4801 caaattatat ttttaaaata ttacttcaag tgtatatcat gtataattac acggcataca
     4861 aaaaaaaaac tcgtcaataa ataaatagaa tttattttat taactacagg aatgtgatgg
     4921 ccgcgttgct ggcgttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4981 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5041 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5101 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5161 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5221 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5281 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5341 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5401 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5461 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5521 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5581 ttaagggatt ttggtcatga gattatcaaa gaagatcttc tagatccagg tacgacatta
     5641 tacttgttat gttttacaaa tgaattggta acaacacttg aatttatcaa aaaatatcca
     5701 tcaattattt ctgatattct tatttatggt tgttctggag caattggaca attgtttatt
     5761 ttccatacat tagaaaaata tggcagttta atcttaataa ctatcacact tacaagaaaa
     5821 atgctaacgc tattattaag tcttatttgg tttaatcata agttaacaat tggccagtgg
     5881 ataggtgtag tattggtatt ttatggagca agtttggaag catatataaa acatacacaa
     5941 ttagctaaaa aaaaagtata aaacatttat acgaaatctc atacgtaaat ttataaaatt
     6001 tataataaac atataaataa tataaaaatg aaacaatatt ctaaaaacaa atctataaca
     6061 aaaaacatat attttaatta cgctacataa ttaatatata atagcaatac catcaatata
     6121 ctgcttcctt aattacgagt atacttaaga ggtatcaatt cctcaatagt tgttttaaca
     6181 agtttaccat tcatctcaat aagaacaaaa caatctggcg cataatctga tataagctct
     6241 cgacacatac cacatggaga aacaacacga atagacctat caacttcatc agaataagga
     6301 tgtcgaactg caacaatagt atcaaaatcc ttttgtccat tagatactgc acttccaata
     6361 gctattgcct ctgcacaaac agtaacacgt cctatataag cttcaatgtg aacagctgat
     6421 ataatctctc cagtctttgt tctaattgct gctccaacat ggtgtttatt atcctcataa
     6481 agcattgtaa tcttttctgt agcaacttct actaattcta aatcctgctg agatatatta
     6541 aatgtcttca tctttttttt aatatatttt tttcaaatat ctaaatatta ttttatgtta
     6601 aaatgaattt ttgaacgtcg atattcaaaa aatatcatga ctgtttttat ttttttgacc
     6661 taattttttc agattaagaa taaatattta acattaagat ttttagatta tacaataaag
     6721 acgtcatttc ttatgacaac tagtccaaat aactacataa ttataaaaat tattagaaaa
     6781 atctacgttt ctaaaaccaa ttcaatatta tatatatata tatatagcct tcataattat
     6841 tctagaaaac aatttatttt aatactattc ttttaaagct acaatattca atattatcat
     6901 ctatatcatt attattacat atctttaata tttttcagaa aaataattat aaaaataaaa
     6961 cgtgccatca tattttttat tgaaattaaa tctatgaagt tttaaatcaa tctaaagtat
     7021 atatgagtaa ccggttctga cagcacccag aaacgctggt caaagtaaaa gatgctgaag
     7081 accagttggg tgcgcgggtg ggttacatcg aactggatct caatagcggt aagatccttg
     7141 agagttttcg ccccgaagaa cgtttcccaa tgatgagcac ctttaaagtt ctgctgtgtg
     7201 gcgcggtgtt atcccgtatt gacgccggtc aagagcagct cggtcgccgc attcactatt
     7261 cgcagaatga cttggttgaa tatagtccgg tcacagaaaa gcatctgacg gatggcatga
     7321 cagtcagaga actgtgctca gctgccataa ccatgagcga taacactgcg gccaacttac
     7381 ttctgaccac gatcggagga ccgaaagaac tgaccgcctt cttgcacaac atgggggatc
     7441 atgtgactcg cttagatcgt tgggaaccgg agctgaatga agcgataccg aacgacgagc
     7501 gtgacaccac gatgcctgtg gcaatggcaa caaccttgcg caaactatta actggcgaac
     7561 ttctgactct cgcttcccgg caacaactga ttgactggat ggaagcggat aaagttgcag
     7621 gacctctgct gcgctcggcc cttccggctg gctggtttat tgcggataaa tctggcgccg
     7681 gggagcgtgg ctctcgcggt attattgcag cgctggggcc ggatggtaaa cccagtcgta
     7741 tcgtagtgat ctacacgacc ggcagccagg caaccatgga tgaacgaaat aggcagatcg
     7801 cggagattgg cgcctcactg attaagcatt ggtaaagcaa aaacaggaag gcaaaatgcc
     7861 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7921 ttcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     7981 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     8041 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
     8101 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     8161 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
     8221 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     8281 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
     8341 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
     8401 gggatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
     8461 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
     8521 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
     8581 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
     8641 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
     8701 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
     8761 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
     8821 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat agtagttcta
     8881 gaatttttgt taaaatttta tttcaagagt tgatgggagt tcttggtata cgcgaattag
     8941 atgaacgttt gaaagatcca cttttacaag agactctttc taatatgttt cctcgggata
     9001 atccaaaaaa tactagattt gcaataaatt attttacttc tattggatta ggattgataa
     9061 ctgtttcatt gcgtgagcat ttggaaacta tcaaggtaaa gctttgaaaa tatagtatta
     9121 actctaattt ctttagaata ctgttacagt aaatcgaagt ctttctaaat cttcatcatc
     9181 ttcaattata tcttcctata ctacatcata ttcatctgaa tctttatcat atcccaatga
     9241 tacttcagag tcagaatcat tgaatcgttc aagatctcgt catcggtcac ctgctcgtca
     9301 tagaatgaga tcccctcgag ataatcgttc aaggccacgt tcatgttcat taaatcgttc
     9361 tagattgcaa tcttctgagt cggaatcatt gaatcgttcg agatctcgtc atcggtcacc
     9421 tccgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg
     9481 cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt
     9541 ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcatgaatc agaaatttgg
     9601 ggataatggg aattttttac tatatacaaa ggaccagctc a
//
