LOCUS       sequence-47995-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Perez-Pinera P, Ousterout DG, Brown MT, Gersbach CA
  TITLE     Gene targeting to the ROSA26 locus directed by engineered zinc 
            finger nucleases.
  JOURNAL   Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14.
  PUBMED    22169954
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-04-1"; volume: "40(8)"; pages: "3741-52. 
            Epub 2011 Dec 14"
COMMENT     Sequence Label: pCMV-RosaL6 ELD mutations
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    61..195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(85..104)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     139..158
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      199..208
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taaccaagtt cctctttcag aggttatttc aggccatggt gctgcgccga attaattccc
       61 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      121 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
      181 tgcaataaac aagttgggcc atggcggcca agcttctgca ggtcgactct agattagtta
      241 attaatcaga agttgatctc gccgttgttg aacttgcgcc gcacctcctc cagtgtcagg
      301 gtgccggctt tgatcatctc gccgccgatc agcagctcct ccacgctcag cacggcgcca
      361 ttgcagttgg tgatgtggtt cagcctggtc agctgggcct tgtagttgcc cttgaagtgg
      421 ccgctcacga acaggaactt gaactcggtc acgctgctag ggtacacctt ccaccactcg
      481 ttggggttga ggtgcttatc ccgtgtctgg ttctcctcca cgtatctctc catctcgtcg
      541 gcctggccga taggcagatt gtagccgccg ctgtaggcct ttgtgtccac gatcacgccg
      601 taatcgatgg ggctgcccac tgtatagatg gcgccgtcag gctttctgct tccgcccagg
      661 tgctctcccc tgtagccgta caccttcatg aagaactcca tcaccttcat ctccaggatg
      721 cggtcctggg tggggttcct ggcgatctcg atcagctcga tgtactcgtg gggcacgtac
      781 ttcagcttgt gccgcagctc ggacttcttc tcctcc
//
