LOCUS       sequence-47997-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2275
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             75..140
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             147..167
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 tggctagcac catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt
      121 acaaggatga cgatgacaag atggccccca agaagaagag gaaggtgggc attcaccgcg
      181 gggtacctat ggtggacttg aggacactcg gttattcgca acagcaacag gagaaaatca
      241 agcctaaggt caggagcacc gtcgcgcaac accacgaggc gcttgtgggg catggcttca
      301 ctcatgcgca tattgtcgcg ctttcacagc accctgcggc gcttgggacg gtggctgtca
      361 aataccaaga tatgattgcg gccctgcccg aagccacgca cgaggcaatt gtaggggtcg
      421 gtaaacagtg gtcgggagcg cgagcacttg aggcgctgct gactgtggcg ggtgagctta
      481 gggggcctcc gctccagctc gacaccgggc agctgctgaa gatcgcgaag agagggggag
      541 taacagcggt agaggcagtg cacgcctggc gcaatgctct caccggggcc cccttgaacc
      601 tgaccccaga ccaggtagtc gcaatcgcgt cacatgacgg gggaaagcaa gccctggaaa
      661 ccgtgcaaag gttgttgccg gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg
      721 tggccattgc aaataataac ggtggcaaac aggctcttga gacggttcag agacttctcc
      781 cagttctctg tcaagcccac gggctgactc ccgatcaagt tgtagcgatt gcgtcgcatg
      841 acgga
//
