LOCUS       sequence-47998-e         552 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Perez-Pinera P, Ousterout DG, Brown MT, Gersbach CA
  TITLE     Gene targeting to the ROSA26 locus directed by engineered zinc 
            finger nucleases.
  JOURNAL   Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14.
  PUBMED    22169954
REFERENCE   2  (bases 1 to 552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-04-1"; volume: "40(8)"; pages: "3741-52. 
            Epub 2011 Dec 14"
COMMENT     Sequence Label: pCMV-RosaR4 KKR mutations
FEATURES             Location/Qualifiers
     source          1..552
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..55
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctacgcgaat tcccctggga tcccaggcat ggggcccaaa aagaaacgca aagttgggcg
       61 cctcgagccc ggggagaaac catacaaatg ccccgagtgt ggaaagtcat ttagtgatcc
      121 aggcgcatta gtaagacatc agcggacaca taccggggag aaaccttata aatgcccaga
      181 atgcgggaaa tcgttcagtc aaagagcaca tttagaaaga catcaacgga cccacaccgg
      241 ggagaagcca tacaagtgtc ccgaatgcgg gaagtcattc tccagaagtg acgatttagt
      301 aagacatcag cgcacgcaca ccggggaaaa gccatataaa tgccccgagt gcggcaaatc
      361 attcagcaga agcgaccacc tgaccaccca ccagcgcacc cataccggtg cggccgcccg
      421 cgccctggtg aagagcgagc tggaggagaa gaagtccgag ctgcggcaca agctgaagta
      481 cgtgccccac gagtacatcg agctgatcga gatcgccagg aaccccaccc aggaccgcat
      541 cctggagatg aa
//
