LOCUS       sequence-48000-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Perez-Pinera P, Ousterout DG, Brown MT, Gersbach CA
  TITLE     Gene targeting to the ROSA26 locus directed by engineered zinc 
            finger nucleases.
  JOURNAL   Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14.
  PUBMED    22169954
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-04-1"; volume: "40(8)"; pages: "3741-52. 
            Epub 2011 Dec 14"
COMMENT     Sequence Label: pCMV-RosaR4 KKR mutations
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    63..197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(87..106)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     141..160
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      201..210
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctaaccaag ttcctctttc agaggttatt tcaggccatg gtgctgcgcc gaattaattc
       61 ccgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
      121 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
      181 gctgcaataa acaagttggg ccatggcggc caagcttctg caggtcgact ctagattagt
      241 tataatcaga agttgatctc gccgttgttg aacttgcgcc gcacctcctc cagtgtcagg
      301 gtgccggctt tgatcatctc gccgccgatc agcagctcct ccacgctcag cacggcgcca
      361 ttgcagttgg ttttgcggtt cagcctggtc agctgggcct tgtagttgcc cttgaagtgg
      421 ccgctcacga acaggaactt gaactcggtc acgctgctag ggtacacctt ccaccactcg
      481 ttggggttga tgtgcttatt ccgggtctgg ttctccttca cgtatctctg catctcgtcg
      541 gcctggccga taggcagatt gtagccgccg ctgtaggcct ttgtgtccac gatcacgccg
      601 taatcgatgg ggctgcccac tgtatagatg gcgccgtcag gctttctgct tccgcccagg
      661 tgctctcccc tgtagccgta caccttcatg aagaactcca tcaccttcat ctccaggatg
      721 cggtcctggg tggggttcct ggcgatctcg atcagctcga tgtactcgtg gggcacgtac
      781 ttcagcttgt gccgcagctc ggacttcttc tcctccagct cgctctt
//
