LOCUS       sequence-48004-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Gyenis L, Duncan JS, Turowec JP, Bretner M, Litchfield DW
  TITLE     Unbiased functional proteomics strategy for protein kinase inhibitor
            validation and identification of bona fide protein kinase 
            substrates: application to identification of EEF1D as a substrate 
            for CK2.
  JOURNAL   J Proteome Res. 2011 Nov 4;10(11):4887-901. Epub 2011 Oct 13.
  PUBMED    21936567
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Proteome 
            Res."; date: "2011-11-4"; volume: "10(11)"; pages: "4887-901. Epub 
            2011 Oct 13"
COMMENT     Sequence Label: pFLAG-EEF1D (pcDNA 3.1+) LG3-1
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             111..134
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttatg gactacaaag
      121 acgatgacga caagatggct acaaacttcc tagcacatga gaagatctgg ttcgacaagt
      181 tcaaatatga cgacgcagaa aggagattct acgagcagat gaacgggcct gtggcaggtg
      241 cctcccgcca ggagaacggc gccagcgtga tcctccgtga cattgcgaga gccagagaga
      301 acatccagaa atccctggct ggaagctcag gccccggggc ctccagcggc accagcggag
      361 accacggtga gctcgtcgtc cggattgcca gtctggaagt ggagaaccag agtctgcgtg
      421 gcgtggtaca ggagctgcag caggccatct ccaagctgga ggcccggctg aacgtgctgg
      481 agaagagctc gcctggccac cgggccacgg ccccacagac ccagcacgta tctcccatgc
      541 gccaagtgga gcccccagcc aagaagccag ccacaccagc agaggatgac gaggatgatg
      601 acattgacct gtttggcagt gacaatgagg aggaggacaa ggaggcggca cagctgcggg
      661 aggagcggct acggcagtac gcggagaaga aggccaagaa gcctgcactg gtggccaagt
      721 cctccatcct gctggatgtc aagccttggg atgatgagac ggacatggcc cagctggagg
      781 cctgtgtgcg ctctatccag ctggacgggc tggtctgggg ggcttccaag ctggtgcccg
      841 tgggctacgg tatccggaag ctacagattc agtgtgtggt ggaggacga
//
