LOCUS       sequence-48013-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2307
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..114
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             121..141
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aattaatacg actcacttat agggagaccc aagctggcta gcaccatgga ctacaaagac
       61 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagatggcc
      121 cccaagaaga agaggaaggt gggcattcac cgcggggtac ctatggtgga cttgaggaca
      181 ctcggttatt cgcaacagca acaggagaaa atcaagccta aggtcaggag caccgtcgcg
      241 caacaccacg aggcgcttgt ggggcatggc ttcactcatg cgcatattgt cgcgctttca
      301 cagcaccctg cggcgcttgg gacggtggct gtcaaatacc aagatatgat tgcggccctg
      361 cccgaagcca cgcacgaggc aattgtaggg gtcggtaaac agtggtcggg agcgcgagca
      421 cttgaggcgc tgctgactgt ggcgggtgag cttagggggc ctccgctcca gctcgacacc
      481 gggcagctgc tgaagatcgc gaagagaggg ggagtaacag cggtagaggc agtgcacgcc
      541 tggcgcaatg cgctcaccgg ggcccccttg aacctgaccc cagaccaggt agtcgcaatc
      601 gcgtcgaaca ttgggggaaa gcaagccctg gaaaccgtgc aaaggttgtt gccggtcctt
      661 tgtcaagacc acggccttac accggagcaa gtcgtggcca ttgcaaataa taacggtggc
      721 aaacaggctc ttgagacggt tcagagactt ctcccagttc tctgtcaagc ccacgggctg
      781 actcccgatc aagttgtagc gattgcgtcg catgacggag gg
//
