LOCUS       sequence-480228-        6634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' AAVLINK plasmid for IQGAP3 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Zhonghua Lu Lab
  TITLE     AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery 
            in Gene Therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-5' IQGAP3
FEATURES             Location/Qualifiers
     source          1..6634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      4112..4567
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4199..4218)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4409..4430
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4584..4603)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4703..4725
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4763..4781)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4849..4953
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4954..5022,5023..5814)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5172..5191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5985..6573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6474..6493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctgaga ggagagcagc gggcccaggc tgggcagcct atgaacgcct
      781 cacagctgag gagatggatg agcagaggcg gcagaatgtt gcctatcagt acctgtgccg
      841 gctggaggag gccaagcgct ggatggaggc ctgcctgaag gaggagcttc cttccccggt
      901 ggagctggag gagagccttc ggaatggagt gctgctggcc aagctgggcc actgttttgc
      961 accctccgtg gttccgttga agaagatcta cgatgtggag cagctgcggt accaggcaac
     1021 tggcttacat ttccgtcaca cagacaacat caacttttgg ctatctgcaa tagcccacat
     1081 cggtctgcct tcgaccttct tcccagagac cacggacatc tatgacaaaa agaacatgcc
     1141 ccgggtagtc tactgcatcc atgctctcag tctcttcctc ttccggctgg gattggcccc
     1201 tcagatacat gatctatacg ggaaagtgaa attcacagct gaggaactca gcaacatggc
     1261 gtccgaactg gccaaatatg gcctccagct gcctgccttc agcaagatcg ggggcatctt
     1321 ggccaatgag ctctcggtgg atgaggctgc agtccatgca gctgttcttg ccatcaatga
     1381 agcagtggag cgaggggtgg tggaggacac cctggctgcc ttgcagaatc ccagtgctct
     1441 tctggagaat ctccgagagc ctctggcagc cgtctaccag gagatgctgg cccaggccaa
     1501 gatggagaag gcagccaatg ccaggaacca tgatgacaga gaaagccagg acatctatga
     1561 ccactaccta actcaggctg aaatccaggg caatatcaac catgtcaacg tccatggggc
     1621 tctagaagtt gttgatgatg ccctggaaag acagagccct gaagccttgc tcaaggccct
     1681 tcaagaccct gccctggccc tgcgaggggt gaggagagac tttgctgact ggtacctgga
     1741 gcagctgaac tcagacagag agcagaaggc acaggagctg ggcctggtgg agcttctgga
     1801 aaaggaggaa gtccaggctg gtgtggctgc agccaacaca aagggtgatc aggaacaagc
     1861 catgctccac gctgtgcagc ggatcaacaa agccatccgg aggggagtgg cggctgacac
     1921 tgtgaaggag ctgatgtgcc ctgaggccca gctgcctcca ctgtaccctg ttgcatcgtc
     1981 tatgtaccag ctggagctgg cagtgctcca gcagcagcag ggggagcttg gccaggagga
     2041 gctcttcgtg gctgtggaga tgctctcagc tgtggtcctg attaaccggg ccctggaggc
     2101 ccgggatgcc agtggcttct ggagcagcct ggtgaaccct gccacaggcc tggctgaggt
     2161 ggaaggagaa aatgcccagc gttacttcga tgccctgctg aaattgcgac aggagcgtgg
     2221 gatgggtgag gacttcctga gctggaatga cctgcaggcc accgtgagcc aggtcaatgc
     2281 acagacccag gaagagactg accgggtcct tgcagtcagc ctcatcaatg aggctctgga
     2341 caaaggcagc cctgagaaga ctctgtctgc cctactgctt cctgcagctg gcctagatga
     2401 tgtcagcctc cctgtcgccc ctcggtacca tctcctcctt gtggcagcca aaaggcagaa
     2461 ggcccaggtg acaggggatc ctggagctgt gctgtggctt gaggagatcc gccagggagt
     2521 ggtcagagcc aaccaggaca ctaatacagc tcagagaatg gctcttggtg tggctgccat
     2581 caatcaagcc atcaaggagg gcaaggcagc ccagactgag cgggtgttga ggaaccccgc
     2641 agtggccctt cgaggggtag ttcccgactg tgccaacggc taccagcgag ccctggaaag
     2701 tgccatggca aagaaacagc gtccagcaga cacagctttc tgggttcaac atgacatgaa
     2761 ggatggcact gcctactact tccatctgca gaccttccag gggatctggg agcaacctcc
     2821 tggctgcccc ctcaacacct ctcacctgac ccgggaggag atccagtcag ctgtcaccaa
     2881 ggtcactgct gcctatgacc gccaacagct ctggaaagcc aacgtcggct ttgttatcca
     2941 gctccaggcc cgcctccgtg gcttcctagt tcggcagaag tttgctgagc attcccactt
     3001 tctgaggacc tggctcccag cagtcatcaa gatccaggct cattggcggg gttataggca
     3061 gcggaagatt tacctggagt ggttgcagta ttttaaagca aacctggatg ccataatcaa
     3121 gatccaggcc tgggcccgga tgtgggcagc tcggaggcaa tacctgaggc gtctgcacta
     3181 cttccagaag aatgttaact ccattgtgaa gatccaggca tttttccgag ccaggaaagc
     3241 ccaagatgac tacaggatat tagtgcatgc accccaccct cctctcagtg tggtacgcag
     3301 atttgcccat ctcttgaatc aaagccagca agacttcttg gctgaggcag agctgctgaa
     3361 gctccaggaa gaggtagtta ggaagatccg atccaatcag cagctggagc aggacctcaa
     3421 catcatggac atcaagattg gcctgctggt gaagaaccgg atcactctgc aggaagtggt
     3481 ctcccactgc aagaagctga ccaagaggaa taaggaacag ctgtcagata tgatggttct
     3541 ggacaagcag aagggtttaa agtcgctgag caaagagaaa cggcagaaac tagaagcata
     3601 ccaacacctc ttctacctgc tccagactca gcccatctac ctggccaagc tgatctttca
     3661 gatgccacag aacaaaacca ccaagttcat ggaggcagtg attttcagcc tgtacaacta
     3721 tgcctccagc cgccgagagg cctatctcct gctccagctg ttcaagacag cactccagga
     3781 ggaaatcaag tcaaaggtaa gtatcaaggt tacaagacag gtttaaggag accaataagc
     3841 tattattcgt ataggatact ttatacgaag ttattaagtt atggagaccg gtgtggcacg
     3901 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     3961 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg gcctcagtga gcgagcgagc
     4021 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4081 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4141 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     4201 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4261 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4321 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4381 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4441 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     4501 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4561 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4621 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4681 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4741 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4801 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4861 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4921 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4981 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5041 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5101 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5161 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5221 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5281 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5341 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5401 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5461 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5521 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5581 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5641 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5701 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5761 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5821 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5881 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5941 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6001 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6061 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6121 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6181 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6241 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6301 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6361 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6421 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6481 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6541 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6601 ttcctggcct tttgctggcc ttttgctcac atgt
//
