LOCUS       sequence-480230-        6413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' AAVLINK plasmid for SMARCA2 expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Zhonghua Lu Lab
  TITLE     AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery 
            in Gene Therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-5' SMARCA2
FEATURES             Location/Qualifiers
     source          1..6413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      3891..4346
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3978..3997)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4188..4209
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4363..4382)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4482..4504
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4542..4560)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4628..4732
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4733..4801,4802..5593)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4951..4970)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5764..6352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6253..6272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatcttcca cgcccacaga ccctggtgcg atgccccacc cagggccttc
      781 gccggggcct gggccttccc ctgggccaat tcttgggcct agtccaggac caggaccatc
      841 cccaggttcc gtccacagca tgatggggcc aagtcctgga cctccaagtg tctcccatcc
      901 tatgccgacg atggggtcca cagacttccc acaggaaggc atgcatcaaa tgcataagcc
      961 catcgatggt atacatgaca aggggattgt agaagacatc cattgtggat ccatgaaggg
     1021 cactggtatg cgaccacctc acccaggcat gggccctccc cagagtccaa tggatcaaca
     1081 cagccaaggt tatatgtcac cacacccatc tccattagga gccccagagc acgtctccag
     1141 ccctatgtct ggaggaggcc caactccacc tcagatgcca ccaagccagc cgggggccct
     1201 catcccaggt gatccgcagg ccatgagcca gcccaacaga ggtccctcac ctttcagtcc
     1261 tgtccagctg catcagcttc gagctcagat tttagcttat aaaatgctgg cccgaggcca
     1321 gcccctcccc gaaacgctgc agcttgcagt ccaggggaaa aggacgttgc ctggcttgca
     1381 gcaacaacag cagcagaagc agagccgcat cagccccatc cagaaaccgc aaggcctgga
     1441 ccccgtggaa attctgcaag agcgggaata cagacttcag gcccgcatag ctcataggat
     1501 acaagaactg gaaaatctgc ctggctcttt gccaccagat ttaagaacca aagcaaccgt
     1561 ggaactaaaa gcacttcggt tactcaattt ccagcgtcag ctgagacagg aggtggtggc
     1621 ctgcatgcgc agggacacga ccctggagac ggctctcaac tccaaagcat acaaacggag
     1681 caagcgccag actctgagag aagctcgcat gaccgagaag ctggagaagc agcagaagat
     1741 tgagcaggag aggaaacgcc gtcagaaaca ccaggaatac ctgaacagta ttttgcaaca
     1801 tgcaaaagat tttaaggaat atcatcggtc tgtggccgga aagatccaga agctctccaa
     1861 agcagtggca acttggcatg ccaacactga aagagagcag aagaaggaga cagagcggat
     1921 tgaaaaggag agaatgcggc gactgatggc tgaagatgag gagggttata gaaaactgat
     1981 tgatcaaaag aaagacaggc gtttagctta ccttttgcag cagaccgatg agtatgtagc
     2041 caatctgacc aatctggttt gggagcacaa gcaagcccag gcagccaaag agaagaagaa
     2101 gaggaggagg aggaagaaga aggctgagga gaatgcagag ggtggggagt ctgccctggg
     2161 accggatgga gagcccatag atgagagcag ccagatgagt gacctccctg tcaaagtgac
     2221 tcacacagaa accggcaagg ttctgttcgg accagaagca cccaaagcaa gtcagctgga
     2281 cgcctggctg gaaatgaatc ctggttatga agttgcccct agatctgaca gtgaagagag
     2341 tgattctgat tatgaggaag aggatgagga agaagagtcc agtaggcagg aaaccgaaga
     2401 gaaaatactc ctggatccaa atagcgaaga agtttctgag aaggatgcta agcagatcat
     2461 tgagacagct aagcaagacg tggatgatga atacagcatg cagtacagtg ccaggggctc
     2521 ccagtcctac tacaccgtgg ctcatgccat ctcggagagg gtggagaaac agtctgccct
     2581 cctaattaat gggaccctaa agcattacca gctccagggc ctggaatgga tggtttccct
     2641 gtataataac aacttgaacg gaatcttagc cgatgaaatg gggcttggaa agaccataca
     2701 gaccattgca ctcatcactt atctgatgga gcacaaaaga ctcaatggcc cctatctcat
     2761 cattgttccc ctttcgactc tatctaactg gacatatgaa tttgacaaat gggctccttc
     2821 tgtggtgaag atttcttaca agggtactcc tgccatgcgt cgctcccttg tcccccagct
     2881 acggagtggc aaattcaatg tcctcttgac tacttatgag tatattataa aagacaagca
     2941 cattcttgca aagattcggt ggaaatacat gatagtggac gaaggccacc gaatgaagaa
     3001 tcaccactgc aagctgactc aggtcttgaa cactcactat gtggccccca gaaggatcct
     3061 cttgactggg accccgctgc agaataagct ccctgaactc tgggccctcc tcaacttcct
     3121 cctcccaaca atttttaaga gctgcagcac atttgaacaa tggttcaatg ctccatttgc
     3181 catgactggt gaaagggtgg acttaaatga agaagaaact atattgatca tcaggcgtct
     3241 acataaggtg ttaagaccat ttttactaag gagactgaag aaagaagttg aatcccagct
     3301 tcccgaaaaa gtggaatatg tgatcaagtg tgacatgtca gctctgcaga agattctgta
     3361 tcgccatatg caagccaagg ggatccttct cacagatggt tctgagaaag ataagaaggg
     3421 gaaaggaggt gctaagacac ttatgaacac tattatgcag ttgagaaaaa tctgcaacca
     3481 cccatatatg tttcagcaca ttgaggaatc ctttgctgaa cacctaggct attcaaatgg
     3541 ggtcatcaat ggggctgaac tgtatcgggc ctcaggtaag tatcaaggtt acaagacagg
     3601 tttaaggaga ccaataagct attattcgta taggatactt tatacgaagt tattaagtta
     3661 tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     3721 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccgg
     3781 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     3841 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     3901 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3961 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4021 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4081 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     4141 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4201 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     4261 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4321 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     4381 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4441 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4501 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4561 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4621 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4681 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4741 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4801 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4861 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4921 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4981 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5041 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5101 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5161 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5221 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5281 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5341 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5401 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5461 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5521 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5581 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5641 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5701 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5761 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5821 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5881 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5941 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6001 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6061 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6121 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6181 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6241 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6301 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6361 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
