LOCUS       sequence-48024-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Acker MG, Kolitz SE, Mitchell SF, Nanda JS, Lorsch JR
  TITLE     Reconstitution of yeast translation initiation.
  JOURNAL   Methods Enzymol. 2007;430:111-45.
  PUBMED    17913637
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2007"; volume: "430"; pages: "111-45"
COMMENT     Sequence Label: pUC19-HHtRNA
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(134..153)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(135..153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(206..222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(206..222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(219..241)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    230..246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(254..260,261..278,279..284))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    299..320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(437..454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(688..707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cattagcttc ctggtagcgc cgctcggttt cgatccgagg acatcagggt tatgagccct
       61 gcgcgcttcc actgcgccac ggcgctgacg gtaccgggta ccgtttcgtc ctcacggact
      121 catcagagcg tctccctata gtgagtcgta ttaaggggga tcctctagag tcgacctgca
      181 ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      241 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      301 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      361 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      421 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      481 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      541 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      601 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      661 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      721 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      781 cgggaagcgt ggc
//
