LOCUS       sequence-48025-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Mitchell SF, Walker SE, Algire MA, Park EH, Hinnebusch AG, Lorsch JR
  TITLE     The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to 
            stimulate canonical translation initiation and to block an 
            alternative pathway.
  JOURNAL   Mol Cell. 2010 Sep 24;39(6):950-62.
  PUBMED    20864040
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2010-09-24"; volume: "39(6)"; pages: "950-62"
COMMENT     Sequence Label: pUC19-RPL41A
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(359..375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(359..375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(372..394)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    383..399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(407..413,414..431,432..437))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    452..473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(590..607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cacatcgatt caatcgaaat gagagccaag tggagaaaga agagaactag aagacttaag
       61 agaaagagac ggaaggtgag agccagatcc aaataagcgg attatgagta aataactcta
      121 attttgtttt aaattctttc aagagtatcg taatgtcatt gatgaattaa catgttagtt
      181 tctattctac ctcataatgg atctaaattg catactaatc tcacggtggg gtgtaaacca
      241 ttgcctacta tttatatagt gctttatata tgtctcacat agtttaatca attgtccgtt
      301 tttttgaaaa ggatcctcta gagtcgacct gcaggcatgc aagcttggcg taatcatggt
      361 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      421 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      481 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      541 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      601 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      661 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      721 aaaaggccag gaaccgtaaa aaggccgcgt tgct
//
