LOCUS       sequence-48034-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2472
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             163..183
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcaccatg gactacaaag accatgacgg tgattataaa
      121 gatcatgaca tcgattacaa ggatgacgat gacaagatgg cccccaagaa gaagaggaag
      181 gtgggcattc accgcggggt acctatggtg gacttgagga cactcggtta ttcgcaacag
      241 caacaggaga aaatcaagcc taaggtcagg agcaccgtcg cgcaacacca cgaggcgctt
      301 gtggggcatg gcttcactca tgcgcatatt gtcgcgcttt cacagcaccc tgcggcgctt
      361 gggacggtgg ctgtcaaata ccaagatatg attgcggccc tgcccgaagc cacgcacgag
      421 gcaattgtag gggtcggtaa acagtggtcg ggagcgcgag cacttgaggc gctgctgact
      481 gtggcgggtg agcttagggg gcctccgctc cagctcgaca ccgggcagct gctgaagatc
      541 gcgaagagag ggggagtaac agcggtagag gcagtgcacg cctggcgcaa tgcgctcacc
      601 ggggccccct tgaacctgac cccagaccag gtagtcgcaa tcgcgaacaa taatggggga
      661 aagcaagccc tggaaaccgt gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt
      721 acaccggagc aagtcgtggc cattgcaagc aatgggggtg gcaaacaggc tcttgagacg
      781 gttcagagac ttctcccagt tctctgtcaa gcccacgggc tgactcccga tcaagttgta
      841 gcgattgcgt ccaacggtgg agggaaacaa gcattggaga ctgtccaacg gctccttccc
      901 gtgttgtgtc aagcccacgg tttgacgcct gcacaagtgg tcgccatcgc cagccatgat
      961 g
//
