LOCUS       sequence-48041-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2321
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..158
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             165..185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcacca tggactacaa agaccatgac ggtgattata
      121 aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag aagaagagga
      181 aggtgggcat tcaccgcggg gtacctatgg tggacttgag gacactcggt tattcgcaac
      241 agcaacagga gaaaatcaag cctaaggtca ggagcaccgt cgcgcaacac cacgaggcgc
      301 ttgtggggca tggcttcact catgcgcata ttgtcgcgct ttcacagcac cctgcggcgc
      361 ttgggacggt ggctgtcaaa taccaagata tgattgcggc cctgcccgaa gccacgcacg
      421 aggcaattgt aggggtcggt aaacagtggt cgggagcgcg agcacttgag gcgctgctga
      481 ctgtggcggg tgagcttagg gggcctccgc tccagctcga caccgggcag ctgctgaaga
      541 tcgcgaagag agggggagta acagcggtag aggcagtgca cgcctggcgc aatgcgctca
      601 ccggggcccc cttgaacctg accccagacc aggtagtcgc aatcgcgtca catgacgggg
      661 gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa gaccacggcc
      721 ttacaccgga gcaagtcgtg gccattgcat cccacgacgg tggcaaacag gctcttgaga
      781 cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc gatcaagttg
      841 tagcgattgc gtcgaacatt ggagggaaac aagcattgga gactgtccaa cggctccttc
      901 ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc gccaacaaca
      961 acggcggtaa gcagg
//
