LOCUS       sequence-48045-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Gyenis L, Duncan JS, Turowec JP, Bretner M, Litchfield DW
  TITLE     Unbiased functional proteomics strategy for protein kinase inhibitor
            validation and identification of bona fide protein kinase 
            substrates: application to identification of EEF1D as a substrate 
            for CK2.
  JOURNAL   J Proteome Res. 2011 Nov 4;10(11):4887-901. Epub 2011 Oct 13.
  PUBMED    21936567
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Proteome 
            Res."; date: "2011-11-4"; volume: "10(11)"; pages: "4887-901. Epub 
            2011 Oct 13"
COMMENT     Sequence Label: pEEF1D-FLAG (pcDNA 3.1+) LG6-1
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgg taccatggct acaaacttcc tagcacatga
      121 gaagatctgg ttcgacaagt tcaaatatga cgacgcagaa aggagattct acgagcagat
      181 gaacgggcct gtggcaggtg cctcccgcca ggagaacggc gccagcgtga tcctccgtga
      241 cattgcgaga gccagagaga acatccagaa atccctggct ggaagctcag gccccggggc
      301 ctccagcggc accagcggag accacggtga gctcgtcgtc cggattgcca gtctggaagt
      361 ggagaaccag agtctgcgtg gcgtggtaca ggagctgcag caggccatct ccaagctgga
      421 ggcccggctg aacgtgctgg agaagagctc gcctggccac cgggccacgg ccccacagac
      481 ccagcacgta tctcccatgc gccaagtgga gcccccagcc aagaagccag ccacaccagc
      541 agaggatgac gaggatgatg acattgacct gtttggcagt gacaatgagg aggaggacaa
      601 ggaggcggca cagctgcggg aggagcggct acggcagtac gcggagaaga aggccaagaa
      661 gcctgcactg gtggccaagt cctccatcct gctggatgtc aagccttggg atgatgagac
      721 ggacatggcc cagctggagg cctgtgtgcg ctctatccag ctggacgggc tggtctgggg
      781 ggcttccaag ctggtgcccg tgggctacgg tatccggaag ctacagattc agtgtgtggt
      841 ggaggacga
//
