LOCUS       sequence-48046-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2375
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..38
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             63..128
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             135..155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg gctagcacca
       61 tggactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
      121 atgacaagat ggcccccaag aagaagagga aggtgggcat tcaccgcggg gtacctatgg
      181 tggacttgag gacactcggt tattcgcaac agcaacagga gaaaatcaag cctaaggtca
      241 ggagcaccgt cgcgcaacac cacgaggcgc ttgtggggca tggcttcact catgcgcata
      301 ttgtcgcgct ttcacagcac cctgcggcgc ttgggacggt ggctgtcaaa taccaagata
      361 tgattgcggc cctgcccgaa gccacgcacg aggcaattgt aggggtcggt aaacagtggt
      421 cgggagcgcg agcacttgag gcgctgctga ctgtggcggg tgagcttagg gggcctccgc
      481 tccagctcga caccgggcag ctgctgaaga tcgcgaagag agggggagta acagcggtag
      541 aggcagtgca cgcctggcgc aatgcgctca ccggggcccc cttgaacctg accccagacc
      601 aggtagtcgc aatcgcgaac aataatgggg gaaagcaagc cctggaaacc gtgcaaaggt
      661 tgttgccggt cctttgtcaa gaccacggcc ttacaccgga gcaagtcgtg gccattgcat
      721 cccacgacgg tggcaaacag gctcttgaga cggttcagag acttctccca gttctctgtc
      781 aagcccacgg gctgactccc gatcaagttg ta
//
