LOCUS       sequence-48047-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Gyenis L, Duncan JS, Turowec JP, Bretner M, Litchfield DW
  TITLE     Unbiased functional proteomics strategy for protein kinase inhibitor
            validation and identification of bona fide protein kinase 
            substrates: application to identification of EEF1D as a substrate 
            for CK2.
  JOURNAL   J Proteome Res. 2011 Nov 4;10(11):4887-901. Epub 2011 Oct 13.
  PUBMED    21936567
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Proteome 
            Res."; date: "2011-11-4"; volume: "10(11)"; pages: "4887-901. Epub 
            2011 Oct 13"
COMMENT     Sequence Label: pEEF1D-FLAG (pcDNA 3.1+) LG6-1
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..48)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgctggatat ctgcagaatt ctcacttgtc gtcatcgtct ttgtagtcca tgatcttgtt
       61 gaaagctgcg atatcgacac tctgcacgtg ctcctcaaac ttggtgatct cctcctccag
      121 caagtctgtc cccaccttgt cgtcctccac cacacactga atctgtagct tccggatacc
      181 gtagcccacg ggcaccagct tggaagcccc ccagaccagc ccgtccagct ggatagagcg
      241 cacacaggcc tccagctggg ccatgtccgt ctcatcatcc caaggcttga catccagcag
      301 gatggaggac ttggccacca gtgcaggctt cttggccttc ttctccgcgt actgccgtag
      361 ccgctcctcc cgcagctgtg ccgcctcctt gtcctcctcc tcattgtcac tgccaaacag
      421 gtcaatgtca tcatcctcgt catcctctgc tggtgtggct ggcttcttgg ctgggggctc
      481 cacttggcgc atgggagata cgtgctgggt ctgtggggcc gtggcccggt ggccaggcga
      541 gctcttctcc agcacgttca gccgggcctc cagcttggag atggcctgct gcagctcctg
      601 taccacgcca cgcagactct ggttctccac ttccagactg gcaatccgga cgacgagctc
      661 accgtggtct ccgctggtgc cgctggaggc cccggggcct gagcttccag ccagggattt
      721 ctggatgttc tctctggctc tcgcaatgtc acggaggatc acgctggcgc cgttctcctg
      781 gcgggaggca cctgccacag gcccgttcat ctgctcgtag aatctccttt ctgcgtcgtc
      841 atatttgaac ttgcacaa
//
