LOCUS       sequence-48056-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2262
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             98..163
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             170..190
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag caccatggac tacaaagacc atgacggtga
      121 ttataaagat catgacatcg attacaagga tgacgatgac aagatggccc ccaagaagaa
      181 gaggaaggtg ggcattcacc gcggggtacc tatggtggac ttgaggacac tcggttattc
      241 gcaacagcaa caggagaaaa tcaagcctaa ggtcaggagc accgtcgcgc aacaccacga
      301 ggcgcttgtg gggcatggct tcactcatgc gcatattgtc gcgctttcac agcaccctgc
      361 ggcgcttggg acggtggctg tcaaatacca agatatgatt gcggccctgc ccgaagccac
      421 gcacgaggca attgtagggg tcggtaaaca gtggtcggga gcgcgagcac ttgaggcgct
      481 gctgactgtg gcgggtgagc ttagggggcc tccgctccag ctcgacaccg ggcagctgct
      541 gaagatcgcg aagagagggg gagtaacagc ggtagaggca gtgcacgcct ggcgcaatgc
      601 gctcaccggg gcccccttga acctgacccc agaccaggta gtcgcaatcg cgtcacatga
      661 cgggggaaag caagccctgg aaaccgtgca aaggttgttg ccggtccttt gtcaagacca
      721 cggccttaca ccggagcaag tcgtggccat tgcatcccac gacggtggca aacaggctct
      781 tgagacggtt cagagacttc tcccagttct ctgtcaagcc cacgggctga ctcccgatca
      841 agttgtagcg attgcgtcga acattggagg gaaacaagca ttggagactg tccaacggct
      901 ccttcccgtg ttgtgtcaag cccacggttt gacgcctgca caagtggtcg ccatcgccaa
      961 caacaacggc gg
//
