LOCUS       sequence-48071-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2448
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..161
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             168..188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagca ccatggacta caaagaccat gacggtgatt
      121 ataaagatca tgacatcgat tacaaggatg acgatgacaa gatggccccc aagaagaaga
      181 ggaaggtggg cattcaccgc ggggtaccta tggtggactt gaggacactc ggttattcgc
      241 aacagcaaca ggagaaaatc aagcctaagg tcaggagcac cgtcgcgcaa caccacgagg
      301 cgcttgtggg gcatggcttc actcatgcgc atattgtcgc gctttcacag caccctgcgg
      361 cgcttgggac ggtggctgtc aaataccaag atatgattgc ggccctgccc gaagccacgc
      421 acgaggcaat tgtaggggtc ggtaaacagt ggtcgggagc gcgagcactt gaggcgctgc
      481 tgactgtggc gggtgagctt agggggcctc cgctccagct cgacaccggg cagctgctga
      541 agatcgcgaa gagaggggga gtaacagcgg tagaggcagt gcacgcctgg cgcaatgcgc
      601 tcaccggggc ccccttgaac ctgaccccag accaggtagt cgcaatcgcg tcacatgacg
      661 ggggaaagca agccctggaa accgtgcaaa ggttgttgcc ggtcctttgt caagaccacg
      721 gccttacacc ggagcaagtc gtggccattg caagcaatgg gggtggcaaa caggctcttg
      781 agacggttca gagacttctc ccagttctct gtcaagccca cgggctgact cccgatcaag
      841 ttgtagcga
//
