LOCUS       sequence-48085-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2200
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             90..155
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             162..182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcaccatgg actacaaaga ccatgacggt gattataaag
      121 atcatgacat cgattacaag gatgacgatg acaagatggc ccccaagaag aagaggaagg
      181 tgggcattca ccgcggggta cctatggtgg acttgaggac actcggttat tcgcaacagc
      241 aacaggagaa aatcaagcct aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg
      301 tggggcatgg cttcactcat gcgcatattg tcgcgctttc acagcaccct gcggcgcttg
      361 ggacggtggc tgtcaaatac caagatatga ttgcggccct gcccgaagcc acgcacgagg
      421 caattgtagg ggtcggtaaa cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg
      481 tggcgggtga gcttaggggg cctccgctcc agctcgacac cgggcagctg ctgaagatcg
      541 cgaagagagg gggagtaaca gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg
      601 gggccccctt gaacctgacc ccagaccagg tagtcgcaat cgcgtcacat gacgggggaa
      661 agcaagccct ggaaaccgtg caaaggttgt tgccggtcct ttgtcaagac cacggcctta
      721 caccggagca agtcgtggcc attgcatccc acgacggtgg caaacaggct cttgagacgg
      781 ttcagagact tctcccagtt ctctgtcaag cccacgggct gactcccgat caagttgtag
      841 cgattgcgtc gcatgacgga
//
