LOCUS       sequence-48095-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2287
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..31
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             56..121
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             128..148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagca ccatggacta
       61 caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
      121 gatggccccc aagaagaaga ggaaggtggg cattcaccgc ggggtaccta tggtggactt
      181 gaggacactc ggttattcgc aacagcaaca ggagaaaatc aagcctaagg tcaggagcac
      241 cgtcgcgcaa caccacgagg cgcttgtggg gcatggcttc actcatgcgc atattgtcgc
      301 gctttcacag caccctgcgg cgcttgggac ggtggctgtc aaataccaag atatgattgc
      361 ggccctgccc gaagccacgc acgaggcaat tgtaggggtc ggtaaacagt ggtcgggagc
      421 gcgagcactt gaggcgctgc tgactgtggc gggtgagctt agggggcctc cgctccagct
      481 cgacaccggg cagctgctga agatcgcgaa gagaggggga gtaacagcgg tagaggcagt
      541 gcacgcctgg cgcaatgcgc tcaccggggc ccccttgaac ctgaccccag accaggtagt
      601 cgcaatcgcg tcacatgacg ggggaaagca agccctggaa accgtgcaaa ggttgttgcc
      661 ggtcctttgt caagaccacg gccttacacc ggagcaagtc gtggccattg catcccacga
      721 cggtggcaaa caggctcttg agacggttca gagacttctc ccagttctct gtcaagccca
      781 cgggctgact cccgatcaag ttgtagcgat tgcgaataac aatggaggga aacaagcatt
      841 ggagactgtc caacggctcc ttcccgtgtt gtgtcaagcc cacggtttga cgcctgcaca
      901 agtggtc
//
