LOCUS       sequence-48098-e         537 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2391
FEATURES             Location/Qualifiers
     source          1..537
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..34
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15..33
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             59..124
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             131..151
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcaccatgga
       61 ctacaaagac catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga
      121 caagatggcc cccaagaaga agaggaaggt gggcattcac cgcggggtac ctatggtgga
      181 cttgaggaca ctcggttatt cgcaacagca acaggagaaa atcaagccta aggtcaggag
      241 caccgtcgcg caacaccacg aggcgcttgt ggggcatggc ttcactcatg cgcatattgt
      301 cgcgctttca cagcaccctg cggcgcttgg gacggtggct gtcaaatacc aagatatgat
      361 tgcggccctg cccgaagcca cgcacgaggc aattgtaggg gtcggtaaac agtggtcggg
      421 agcgcgagca cttgaggcgc tgctgactgt ggcgggtgag cttagggggc ctccgctcca
      481 gctcgacacc gggcagctgc tgaagatcgc gaagagaggg ggagtaacag cggtaga
//
