LOCUS       sequence-48106-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2385
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             65..130
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             137..157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagcac
       61 catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
      121 cgatgacaag atggccccca agaagaagag gaaggtgggc attcaccgcg gggtacctat
      181 ggtggacttg aggacactcg gttattcgca acagcaacag gagaaaatca agcctaaggt
      241 caggagcacc gtcgcgcaac accacgaggc gcttgtgggg catggcttca ctcatgcgca
      301 tattgtcgcg ctttcacagc accctgcggc gcttgggacg gtggctgtca aataccaaga
      361 tatgattgcg gccctgcccg aagccacgca cgaggcaatt gtaggggtcg gtaaacagtg
      421 gtcgggagcg cgagcacttg aggcgctgct gactgtggcg ggtgagctta gggggcctcc
      481 gctccagctc gacaccgggc agctgctgaa gatcgcgaag agagggggag taacagcggt
      541 agaggcagtg cacgcctggc gcaatgcgct caccggggcc cccttgaacc tgaccccaga
      601 ccaggtagtc gcaatcgcgt cacatgacgg gggaaagcaa gccctggaaa ccgtgcaaag
      661 gttgttgccg gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg tggccattgc
      721 aagcaacatc ggtggcaaac aggctcttga gacggttcag agacttctcc cagttctctg
      781 tcaagcccac gggctgactc ccgatcaagt tgtagcgatt gcgaataaca atggagggaa
      841 acaagcattg gagactgtcc aacggctcct tcccgtgttg tgtcaagccc acggtttgac
      901 gcctgcacaa gtggtcgcca tcgcca
//
