LOCUS       sequence-48109-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Pasque V, Gillich A, Garrett N, Gurdon JB
  TITLE     Histone variant macroH2A confers resistance to nuclear 
            reprogramming.
  JOURNAL   EMBO J. 2011 May 6;30(12):2373-87. doi: 10.1038/emboj.2011.144.
  PUBMED    21552206
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011"; journalName: "EMBO J"; date: "2011-05-6- 6"; 
            volume: "30"; issue: "12"; pages: "2373-87"
COMMENT     Sequence Label: pENTR macroH2A1.2-C
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     138..154
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     138..154
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     159..178
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        159..177
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    195..294
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
       61 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
      121 acacgggcca gagctgccag gaaacagcta tgaccatgta atacgactca ctatagggga
      181 tatcagctgg atggcaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
      241 aaattgataa gcaatgcttt cttataatgc caactttgta caagaaagct gggtcggcgc
      301 gcccaccctt gttggcgtcc agcttggcca tttcctgcac atagatacct atgctctcac
      361 tgtcaaaaag catgaagtac acagttttga tggaggagga catcgtggag acaaagtagc
      421 tggagatggc cttcagaatg agctgggccg ctgtctgctt cgggaacccg ttcctgccgc
      481 tgccaatgga tgggaaggcg atggatttca gctttctgtc atcagctaga gccaagcagt
      541 ttttcaccgt cttttctaga agttcttcac atttgtctgc accccagaca ggactattac
      601 agtggatcac aaacttggca ggcaggccat ggcctgcact aatagcagct ccagctacct
      661 ccaagggccc gttctttttc cggagttcca gaacagcttc tacaaactcc ttgccgccct
      721 tcttctccag tgtgtttcct aggtcatctt ta
//
