LOCUS       sequence-481112-        2750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  30x 147-bp Widom 601 nucleosome positioning sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Hammonds EF, Singh A, Suresh KK, Yang S, Zahorodny SSM, Gupta R, 
            Potoyan DA, Banerjee PR, Morrison EA
  TITLE     Histone H3 tail charge patterns govern nucleosome condensate 
            formation and dynamics.
  JOURNAL   bioRxiv [Preprint]. 2025 Apr 10:2025.04.09.647968. doi: 
            10.1101/2025.04.09.647968.
  PUBMED    40291647
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Apr 10:2025.04.09.647968. doi: 
            10.1101/2025.04.09.647968."
COMMENT     Sequence Label: pEM184_30x147bp_Widom601
FEATURES             Location/Qualifiers
     source          1..2750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..810
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(68..87)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      957..1545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1446..1465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1699..1716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1877..1904)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1996..2082)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2131..2153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2145..2162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2146..2162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2172..2191
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2172..2190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2530..2546)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2530..2546)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2583..2603)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2583..2601)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2622..2638)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2622..2638)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(join(2740..2750,1..9))
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
       61 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
      121 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
      181 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
      241 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
      301 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
      361 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
      421 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
      481 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
      541 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
      601 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
      661 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
      721 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
      781 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
      841 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
      901 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1081 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1621 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1681 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cgcatatgag cgcccaatac
     1741 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     1801 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     1861 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     1921 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     1981 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     2041 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     2101 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
     2161 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg tacctaacgg agatctgata
     2221 tcgagaatcc cggtgccgag gccgctcaat tggtcgtaga cagctctagc accgcttaaa
     2281 cgcacgtacg cgctgtcccc cgcgttttaa ccgccaaggg gattactccc tagtctccag
     2341 gcacgtgtca gatatataca tccgatatcg gatctgatat cgagaatccc ggtgccgagg
     2401 ccgctcaatt ggtcgtagac agctctagca ccgcttaaac gcacgtacgc gctgtccccc
     2461 gcgttttaac cgccaagggg attactccct agtctccagg cacgtgtcag atatatacat
     2521 ccgatatcgg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
     2581 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctggc
     2641 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     2701 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
//
