LOCUS       sequence-481227-       11959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NanoLuc-CBP Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NanoLuc-CBP Fusion Vector
FEATURES             Location/Qualifiers
     source          1..11959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1065..1577
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     regulatory      6465..6474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9038..9056)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    9093..9214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9104..9123)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9158..9177
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        9459..9816
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9459..9650
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9482..9502)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9667..9802
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9831..9878
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9910..10704
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9964..9983)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10574..10593
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10768..10816
                     /note="synthetic polyadenylation signal"
     rep_origin      10973..11518
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     11419..11438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11635..11652
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggctgagaac ttgctggacg
     1621 gaccgcccaa ccccaaaaga gccaaactca gctcgcccgg tttctcggcg aatgacagca
     1681 cagattttgg atcattgttt gacttggaaa atgatcttcc tgatgagctg atacccaatg
     1741 gaggagaatt aggcctttta aacagtggga accttgttcc agatgctgct tccaaacata
     1801 aacaactgtc ggagcttcta cgaggaggca gcggctctag tatcaaccca ggaataggaa
     1861 atgtgagcgc cagcagcccc gtgcagcagg gcctgggtgg ccaggctcaa gggcagccga
     1921 acagtgctaa catggccagc ctcagtgcca tgggcaagag ccctctgagc cagggagatt
     1981 cttcagcccc cagcctgcct aaacaggcag ccagcacctc tgggcccacc cccgctgcct
     2041 cccaagcact gaatccgcaa gcacaaaagc aagtggggct ggcgactagc agccctgcca
     2101 cgtcacagac tggacctggt atctgcatga atgctaactt taaccagacc cacccaggcc
     2161 tcctcaatag taactctggc catagcttaa ttaatcaggc ttcacaaggg caggcgcaag
     2221 tcatgaatgg atctcttggg gctgctggca gaggaagggg agctggaatg ccgtacccta
     2281 ctccagccat gcagggcgcc tcgagcagcg tgctggctga gaccctaacg caggtttccc
     2341 cgcaaatgac tggtcacgcg ggactgaaca ccgcacaggc aggaggcatg gccaagatgg
     2401 gaataactgg gaacacaagt ccatttggac agccctttag tcaagctgga gggcagccaa
     2461 tgggagccac tggagtgaac ccccagttag ccagcaaaca gagcatggtc aacagtttgc
     2521 ccaccttccc tacagatatc aagaatactt cagtcaccaa cgtgccaaat atgtctcaga
     2581 tgcaaacatc agtgggaatt gtacccacac aagcaattgc aacaggcccc actgcagatc
     2641 ctgaaaaacg caaactgata cagcagcagc tggttctact gcttcatgct cataagtgtc
     2701 agagacgaga gcaagcaaac ggagaggttc gggcctgctc gctcccgcat tgtcgaacca
     2761 tgaaaaacgt tttgaatcac atgacgcatt gtcaggctgg gaaagcctgc caagttgccc
     2821 attgtgcatc ttcacgacaa atcatctctc attggaagaa ctgcacacga catgactgtc
     2881 ctgtttgcct ccctttgaaa aatgccagtg acaagcgaaa ccaacaaacc atcctggggt
     2941 ctccagctag tggaattcaa aacacaattg gttctgttgg cacagggcaa cagaatgcca
     3001 cttctttaag taacccaaat cccatagacc ccagctccat gcagcgagcc tatgctgctc
     3061 tcggactccc ctacatgaac cagccccaga cgcagctgca gcctcaggtt cctggccagc
     3121 aaccagcaca gcctcaaacc caccagcaga tgaggactct caaccccctg ggaaataatc
     3181 caatgaacat tccagcagga ggaataacaa cagatcagca gcccccaaac ttgatttcag
     3241 aatcagctct tccgacttcc ctgggggcca caaacccact gatgaacgat ggctccaact
     3301 ctggtaacat tggaaccctc agcactatac caacagcagc tcctccttct agcaccggtg
     3361 taaggaaagg ctggcacgaa catgtcactc aggacctgcg gagccatcta gtgcataaac
     3421 tcgtccaagc catcttccca acacctgatc ccgcagctct aaaggatcgc cgcatggaaa
     3481 acctggtagc ctatgctaag aaagtggaag gggacatgta cgagtctgcc aacagcaggg
     3541 atgaatatta tcacttatta gcagagaaaa tctacaagat acaaaaagaa ctagaagaaa
     3601 aacggaggtc gcgtttacat aaacaaggca tcttggggaa ccagccagcc ttaccagccc
     3661 cgggggctca gccccctgtg attccacagg cacaacctgt gagacctcca aatggacccc
     3721 tgtccctgcc agtgaatcgc atgcaagttt ctcaagggat gaattcattt aaccccatgt
     3781 ccttggggaa cgtccagttg ccacaagcac ccatgggacc tcgtgcagcc tccccaatga
     3841 accactctgt ccagatgaac agcatgggct cagtgccagg gatggccatt tctccttccc
     3901 gaatgcctca gcctccgaac atgatgggtg cacacaccaa caacatgatg gcccaggcgc
     3961 ccgctcagag ccagtttctg ccacagaacc agttcccgtc atccagcggg gcgatgagtg
     4021 tgggcatggg gcagccgcca gcccaaacag gcgtgtcaca gggacaggtg cctggtgctg
     4081 ctcttcctaa ccctctcaac atgctggggc ctcaggccag ccagctacct tgccctccag
     4141 tgacacagtc accactgcac ccaacaccgc ctcctgcttc cacggctgct ggcatgccat
     4201 ctctccagca cacgacacca cctgggatga ctcctcccca gccagcagct cccactcagc
     4261 catcaactcc tgtgtcgtct tccgggcaga ctcccacccc gactcctggc tcagtgccca
     4321 gtgctaccca aacccagagc acccctacag tccaggcagc agcccaggcc caggtgaccc
     4381 cgcagcctca aaccccagtt cagcccccgt ctgtggctac ccctcagtca tcgcagcaac
     4441 agccgacgcc tgtgcacgcc cagcctcctg gcacaccgct ttcccaggca gcagccagca
     4501 ttgataacag agtccctacc ccctcctcgg tggccagcgc agaaaccaat tcccagcagc
     4561 caggacctga cgtacctgtg ctggaaatga agacggagac ccaagcagag gacactgagc
     4621 ccgatcctgg tgaatccaaa ggggagccca ggtctgagat gatggaggag gatttgcaag
     4681 gagcttccca agttaaagaa gaaacagaca tagcagagca gaaatcagaa ccaatggaag
     4741 tggatgaaaa gaaacctgaa gtgaaagtag aagttaaaga ggaagaagag agtagcagta
     4801 acggcacagc ctctcagtca acatctcctt cgcagccgcg caaaaaaatc tttaaaccag
     4861 aggagttacg ccaggccctc atgccaaccc tagaagcact gtatcgacag gacccagagt
     4921 cattaccttt ccggcagcct gtagatcccc agctcctcgg aattccagac tattttgaca
     4981 tcgtaaagaa tcccatggac ctctccacca tcaagcggaa gctggacaca gggcaatacc
     5041 aagagccctg gcagtacgtg gacgacgtct ggctcatgtt caacaatgcc tggctctata
     5101 atcgcaagac atcccgagtc tataagtttt gcagtaagct tgcagaggtc tttgagcagg
     5161 aaattgaccc tgtcatgcag tcccttggat attgctgtgg acgcaagtat gagttttccc
     5221 cacagacttt gtgctgctat gggaagcagc tgtgtaccat tcctcgcgat gctgcctact
     5281 acagctatca gaataggtat catttctgtg agaagtgttt cacagagatc cagggcgaga
     5341 atgtgaccct gggtgacgac ccttcacagc cccagacgac aatttcaaag gatcagtttg
     5401 aaaagaagaa aaatgatacc ttagaccccg aacctttcgt tgattgcaag gagtgtggcc
     5461 ggaagatgca tcagatttgc gttctgcact atgacatcat ttggccttca ggttttgtgt
     5521 gcgacaactg cttgaagaaa actggcagac ctcgaaaaga aaacaaattc agtgctaaga
     5581 ggctgcagac cacaagactg ggaaaccact tggaagaccg agtgaacaaa tttttgcggc
     5641 gccagaatca ccctgaagcc ggggaggttt ttgtccgagt ggtggccagc tcagacaaga
     5701 cggtggaggt caagcccggg atgaagtcac ggtttgtgga ttctggggaa atgtctgaat
     5761 ctttcccata tcgaaccaaa gctctgtttg cttttgagga aattgacggc gtggatgtct
     5821 gcttttttgg aatgcacgtc caagaatacg gctctgattg cccccctcca aacacgaggc
     5881 gtgtgtacat ttcttatctg gatagtattc atttcttccg gccacgttgc ctccgcacag
     5941 ccgtttacca tgagatcctt attggatatt tagagtatgt gaagaaatta gggtatgtga
     6001 cagggcacat ctgggcctgt cctccaagtg aaggagatga ttacatcttc cattgccacc
     6061 cacctgatca aaaaataccc aagccaaaac gactgcagga gtggtacaaa aagatgctgg
     6121 acaaggcgtt tgcagagcgg atcatccatg actacaagga tattttcaaa caagcaactg
     6181 aagacaggct caccagtgcc aaggaactgc cctattttga aggtgatttc tggcccaatg
     6241 tgttagaaga gagcattaag gaactagaac aagaagaaga ggagaggaaa aaggaagaga
     6301 gcactgcagc cagtgaaacc actgagggca gtcagggcga cagcaagaat gccaagaaga
     6361 agaacaacaa gaaaaccaac aagaacaaaa gcagcatcag ccgcgccaac aagaagaagc
     6421 ccagcatgcc caacgtgtcc aatgacctgt cccagaagct gtatgccacc atggagaagc
     6481 acaaggaggt cttcttcgtg atccacctgc acgctgggcc tgtcatcaac accctgcccc
     6541 ccatcgtcga ccccgacccc ctgctcagct gtgacctcat ggatgggcgc gacgccttcc
     6601 tcaccctcgc cagagacaag cactgggagt tctcctcctt gcgccgctcc aagtggtcca
     6661 cgctctgcat gctggtggag ctgcacaccc agggccagga ccgctttgtc tacacctgca
     6721 acgagtgcaa gcaccacgtg gagacgcgct ggcactgcac tgtgtgcgag gactacgacc
     6781 tctgcatcaa ctgctataac acgaagagcc atgcccataa gatggtgaag tgggggctgg
     6841 gcctggatga cgagggcagc agccagggcg agccacagtc aaagagcccc caggagtcac
     6901 gccggctgag catccagcgc tgcatccagt cgctggtgca cgcgtgccag tgccgcaacg
     6961 ccaactgctc gctgccatcc tgccagaaga tgaagcgggt ggtgcagcac accaagggct
     7021 gcaaacgcaa gaccaacggg ggctgcccgg tgtgcaagca gctcatcgcc ctctgctgct
     7081 accacgccaa gcactgccaa gaaaacaaat gccccgtgcc cttctgcctc aacatcaaac
     7141 acaagctccg ccagcagcag atccagcacc gcctgcagca ggcccagctc atgcgccggc
     7201 ggatggccac catgaacacc cgcaacgtgc ctcagcagag tctgccttct cctacctcag
     7261 caccgcccgg gacccccaca cagcagccca gcacacccca gacgccgcag ccccctgccc
     7321 agccccaacc ctcacccgtg agcatgtcac cagctggctt ccccagcgtg gcccggactc
     7381 agccccccac cacggtgtcc acagggaagc ctaccagcca ggtgccggcc cccccacccc
     7441 cggcccagcc ccctcctgca gcggtggaag cggctcggca gatcgagcgt gaggcccagc
     7501 agcagcagca cctgtaccgg gtgaacatca acaacagcat gcccccagga cgcacgggca
     7561 tggggacccc ggggagccag atggcccccg tgagcctgaa tgtgccccga cccaaccagg
     7621 tgagcgggcc cgtcatgccc agcatgcctc ccgggcagtg gcagcaggcg ccccttcccc
     7681 agcagcagcc catgccaggc ttgcccaggc ctgtgatatc catgcaggcc caggcggccg
     7741 tggctgggcc ccggatgccc agcgtgcagc cacccaggag catctcaccc agcgctctgc
     7801 aagacctgct gcggaccctg aagtcgccca gctcccctca gcagcaacag caggtgctga
     7861 acattctcaa atcaaacccg cagctaatgg cagctttcat caaacagcgc acagccaagt
     7921 acgtggccaa tcagcccggc atgcagcccc agcctggcct ccagtcccag cccggcatgc
     7981 aaccccagcc tggcatgcac cagcagccca gcctgcagaa cctgaatgcc atgcaggctg
     8041 gcgtgccgcg gcccggtgtg cctccacagc agcaggcgat gggaggcctg aacccccagg
     8101 gccaggcctt gaacatcatg aacccaggac acaaccccaa catggcgagt atgaatccac
     8161 agtaccgaga aatgttacgg aggcagctgc tgcagcagca gcagcaacag cagcagcaac
     8221 aacagcagca acagcagcag cagcaaggga gtgccggcat ggctgggggc atggcggggc
     8281 acggccagtt ccagcagcct caaggacccg gaggctaccc accggccatg cagcagcagc
     8341 agcgcatgca gcagcatctc cccctccagg gcagctccat gggccagatg gcggctcaga
     8401 tgggacagct tggccagatg gggcagccgg ggctgggggc agacagcacc cccaacatcc
     8461 agcaagccct gcagcagcgg attctgcagc aacagcagat gaagcagcag attgggtccc
     8521 caggccagcc gaaccccatg agcccccagc aacacatgct ctcaggacag ccacaggcct
     8581 cgcatctccc tggccagcag atcgccacgt cccttagtaa ccaggtgcgg tctccagccc
     8641 ctgtccagtc tccacggccc cagtcccagc ctccacattc cagcccgtca ccacggatac
     8701 agccccagcc ttcgccacac cacgtctcac cccagactgg ttccccccac cccggactcg
     8761 cagtcaccat ggccagctcc atagatcagg gacacttggg gaaccccgaa cagagtgcaa
     8821 tgctccccca gctgaacacc cccagcagga gtgcgctgtc cagcgaactg tccctggtcg
     8881 gggacaccac gggggacacg ctagagaagt ttgtggaggg cttggtttaa acgaattcgg
     8941 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagctga tccggctgct
     9001 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     9061 ccccttgggg cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     9121 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     9181 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     9241 gtttcaggtt cagggggaga tgtgggaggt ttttttaagc aagtaaaacc tctacaaatg
     9301 tggtaaaatc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     9361 ccttacgcat ctgtgcggta tttcacaccg catacgcgga tctgcgcagc accatggcct
     9421 gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg
     9481 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9541 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     9601 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     9661 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9721 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9781 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaattaact gttggcaatt
     9841 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaacccagg
     9901 aggcagatca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     9961 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
    10021 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
    10081 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
    10141 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
    10201 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
    10261 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
    10321 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
    10381 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
    10441 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
    10501 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
    10561 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
    10621 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
    10681 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
    10741 cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt acatctgtgt
    10801 gttggttttt tgtgtgaatc gatagcgata aggatccgct catgcgatga taagctgtca
    10861 aacatgagaa ttacaactta tatcgtatgg ggctgacttc aggtgctaca tttgaagaga
    10921 taaattgcac tgaaatctag aaatatttta tctgattaat aagatgatct tcttgagatc
    10981 gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt
    11041 tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc
    11101 agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact
    11161 cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg
    11221 gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc
    11281 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
    11341 caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
    11401 cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    11461 ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa
    11521 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa
    11581 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag
    11641 tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca
    11701 gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt
    11761 aagccagtat acactccgct agcgctgatg tccggcggtg cttttgccgt tacgcaccac
    11821 cccgtcatga gattacgcgt tttagcggtg cgtacaatta agggattatg gtaaatccac
    11881 ttactgtctg ccctcgtagc catcgagata aaccgcagta ctccggccac gatgcgtccg
    11941 gcgtagagga tcgagatct
//
