LOCUS       sequence-481501-        9029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  +end oriented kinesin for optogenetic pulling experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Pasolli M, Meiring JCM, Conboy JP, Koenderink GH, Akhmanova A
  TITLE     Optogenetic and chemical genetic tools for rapid repositioning of 
            vimentin intermediate filaments.
  JOURNAL   J Cell Biol. 2025 Sep 1;224(9):e202504004. doi: 
            10.1083/jcb.202504004. Epub 2025 Jul 8.
  PUBMED    40627079
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2025-09-1"; volume: "224(9):e202504004. doi"; pages: 
            " 10.1083/jcb.202504004. Epub 2025 Jul 8"
COMMENT     Sequence Label: KIF5A-mVenus-iLID
FEATURES             Location/Qualifiers
     source          1..9029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     941..963
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1026..1035
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2808..2810,2811..3521)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2808..2810,2811..3521)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2850..2871)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3111..3130)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3458..3479
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3621..4058
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     polyA_signal    4188..4322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4225..4244)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4279..4298
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4336..4352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4336..4352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4349..4371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4360..4376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4384..4390,4391..4408,4409..4414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4429..4450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4567..4584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4738..5326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4818..4837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5497..6288,6289..6357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6120..6139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6358..6462)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6530..6548
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6586..6608)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6708..6727
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6921..6943
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6935..6952
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6936..6952
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
       61 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      121 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      181 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      241 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      301 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
      361 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
      421 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
      481 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
      541 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
      601 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
      661 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
      721 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
      781 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
      841 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgtccg
      901 cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
      961 accggcgggg tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg
     1021 ggcgcgccac catggcggag actaacaacg aatgcagcat caaggtgctt tgccgatttc
     1081 ggcccctgaa ccaggccgag attctgcggg gggacaagtt catccccatt ttccaagggg
     1141 acgacagcgt cattattggg ggaaagccat atgtctttga ccgcgtcttc cccccaaaca
     1201 ccactcagga gcaggtttac cacgcctgtg ccatgcagat cgtcaaagac gtccttgctg
     1261 gttacaatgg cacaatcttc gcttatggac agacatcctc agggaaaacg cataccatgg
     1321 aggggaagct gcacgaccct cagctgatgg gcatcattcc ccggatcgct cgagacatct
     1381 tcaaccacat ctactccatg gatgagaacc ttgaattcca cattaaggta tcttacttcg
     1441 agatttacct ggataagatc cgtgaccttt tggatgtgac caagacgaac ctgtccgtgc
     1501 atgaggacaa aaaccgggtg ccgtttgtca agggttgtac cgaacgcttt gtgtccagcc
     1561 cagaggagat tctggatgtg atcgatgagg ggaagtccaa ccgtcacgta gctgtcacca
     1621 acatgaacga gcacagttct cggagccaca gcatcttcct catcaacatc aagcaggaga
     1681 acgtagagac cgagcagaag ctcagcggga agctgtacct cgtggatctg gccggaagcg
     1741 agaaggtcag caagacaggg gcagagggag ccgttctgga cgaggcaaag aatatcaaca
     1801 agtcgctgtc ggccctgggg aacgtgatct ctgcactggc agagggcacc aaaagctacg
     1861 tgccgtaccg cgacagcaaa atgacgagga ttctccagga ctctctggga gggaactgca
     1921 ggactaccat gttcatctgc tgctcgccgt ccagctacaa tgacgcagag accaagtcca
     1981 cgctcatgtt tggacagcgg gcgaagacca tcaagaacac tgcctcagtg aatctggagc
     2041 tgactgctga gcagtggaag aagaagtatg agaaggagaa ggagaagacc aaggcccaga
     2101 aggagacaat tgcgaagcta gaggctgagc ttagccggtg gcgcaatgga gagaatgtgc
     2161 ctgagactga gcgcctggct ggagaggact cagctctggg agctgagctc tgcgaggaga
     2221 cccctgtgaa tgacaactca tccattgtgg tacgcatcgc acctgaggaa aggcagaaat
     2281 atgaggaaga gatccgccgt ctctacaagc agcttgatga caaggatgat gagatcaacc
     2341 agcagagcca gctcattgag aagctgaagc agcagatgct ggaccaggaa gagctgctcg
     2401 tgtccactcg gggagacaac gagaaggtcc agcgggagct tagccacctg cagtccgaga
     2461 acgatgctgc gaaggacgag gtgaaggaag tgctgcaggc cctagaggag ctggcggtca
     2521 actacgacca gaagtcccag gaggtggagg agaagagcca gcagaaccag ctgctggtgg
     2581 acgagctgtc ccagaaagtg gccaccatgc tgtccctgga gtccgagcta cagcggctcc
     2641 aggaggtcag tggacaccag cgaaagcgga tcgctgaggt gctgaatggg ctgatgaggg
     2701 acctgagttc tagaggtggc gggggcagtg ggggaggggg aagtggaggc ggcggttctg
     2761 gcgggggagg ctccggtggc ggggggagcg ggggtgggag taagcttgtg agcaagggcg
     2821 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     2881 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     2941 agttgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctcg
     3001 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     3061 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     3121 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     3181 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     3241 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggcaaact
     3301 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
     3361 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     3421 ccaaactgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     3481 ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggctagcgga ggcggtggct
     3541 caggcggagg agggtcagga gggggaggca gtggcggggg cggctctgga ggagggggtt
     3601 ctggtggtgg tagctacgta ctggcaacca cactggaacg gatcgagaaa aatttcgtga
     3661 ttactgatcc gagactgcct gacaacccaa tcatttttgc gagcgattcc ttcctgcagc
     3721 tgacagaata ttctcgggaa gagatcctgg ggcgcaattg ccgttttctg cagggacccg
     3781 agacagaccg tgccactgtt cggaaaatca gagatgctat tgacaaccag actgaagtga
     3841 ccgttcagct gatcaattat accaagagcg gcaagaagtt ctggaacgtg ttccacctgc
     3901 agccgatgcg cgattataag ggcgacgtcc agtacttcat tggcgtgcag ctggatggca
     3961 ccgaacgtct tcatggcgcc gctgagcgtg aggcggtctg cctgatcaaa aagacagcct
     4021 ttcagattgc tgaggcagcg aacgacgaaa attacttttg aaccggtcct agataagtaa
     4081 tgatcataat cagccatatc acatctgtag aggttttact tgctttaaaa aacctcccac
     4141 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     4201 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     4321 tcaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4381 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4441 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4501 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4561 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4621 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4681 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4741 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4801 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4861 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4921 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4981 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5041 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5101 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5161 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5221 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5281 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5341 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5401 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5461 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5521 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5581 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5641 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5701 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5761 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5821 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5881 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5941 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6001 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6061 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6121 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6181 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6241 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6301 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6361 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6421 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6481 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     6541 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     6601 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     6661 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     6721 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     6781 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     6841 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     6901 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagtt
     6961 gggatctttg cattggccca cggctctcag gatggggatg ctccccttca gcacccggtt
     7021 ccccttggaa actgatggtc ctggctctgt ggcatggcag tggcactgtg aggagccccc
     7081 taccagcagc acacagtggg gttggcactg ccacgctccg gatgccgcgc tctgatccaa
     7141 ccccataatc aagggaaccc gaattgcccc atcattgccc ccaccacccc catcctgccg
     7201 ggccctcaca ccccacgctg ccttctggtg acattcccca gcccaaaccc acggcttcat
     7261 ggctaccgcg gggcatttcc cattgccgcc ccattatcag ctctgcacac ctcccgctgt
     7321 acccatgcct cgtggctgcc cttctttgac gtataatctt ctaattaata cccggccttg
     7381 tcaaagtgga gcacaaacgt taattaattc cccagcaggc aggtaattaa cagtgtgact
     7441 ccctttttgc tgcgagtggg gctgatacag agagatgtgg cactatggag cccacggggt
     7501 cctggcactg ggtgcccacg gaggtcccca tgtgctgcag tgtcaccgcc tccgaggaga
     7561 cagtattgtc cctgcggtgt ccctgcagct cagctctgtc cacagggcca cctccagttt
     7621 ggaggggaca caatgcagcc ccgatgcaac ccatcctcgc agcatcccag ggacaaagac
     7681 cccactgcaa gaccgcacac agggctgggt cccgctcccc taatatctac agtgcttctt
     7741 gcatggcccc ttaatcaatg cagttaatca gcatgcgctc atgcaccgct ctggagctgc
     7801 aaagcccctc gcagcgctgc tcaccaacac cgcgcaccgc cccggcccag cctgcagcac
     7861 gcgctgcaaa caggaaagaa acaaaatatt gcccaaatgt aggcaaaggc attcggctgc
     7921 cttgacctcc gccgggccgg gccctgcctg actcagctcc ttactcagcg ctcgcttcct
     7981 ccctccggct gccaccgccg cagcgcacac cctgacaaag agtggccctt aacgggctct
     8041 gaggtgcacc cagcagtgca ctcagcagtc caagggccgg cctggaggtt tgcaccgcta
     8101 cgtgctgaca ttagcattga acttggccct gggtagtgct gcaggccggg cggggtgggt
     8161 gtagagagtg cagcgcgcgt tgcacccggt gccccttccc ctcccttgca tcccagcagg
     8221 ctgcacccca gcaccaggcc cgtgcatgca tgctcctggt gttattgcag cctggtgcat
     8281 gcatgcgtct tgatggtgca gcgctgtgca tgcatcctcc ttggtgtgta gcagtctagt
     8341 gcatgcatac ccctcggtgt tattgctgct ctgtgcacgc acgctcattg tatcacttca
     8401 tcccagtgca tgcactcaca ctggagcgat tgctgctcgg tgcacgcaca ctcattgtat
     8461 cactgcagct cagtggctgc acgcacaccg gtgttattgc tgctcggtgc gtgcatgcac
     8521 atcagtgtcg ctgcagctca gtgcatgcac gctcattgcc catcgctatc cctgcctctc
     8581 ctgctggcgc tccccgggag gtgacttcaa ggggaccgca ggaccacctc gggggtgggg
     8641 ggagggctgc acacgcggac cccgctcccc ctccccaaca aagcactgtg gaatcaaaaa
     8701 ggggggaggg gggatggagg ggcgcgtcac acccccgccc cacaccctca cctcgaggtg
     8761 agccccacgt tctgcttcac tctccccatc tccccccccc ccccaccccc aattttgtat
     8821 ttatttattt tttaattatt ttgggggggg aggggggggg gggggggggg gcgcgcgcca
     8881 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
     8941 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
     9001 tataaaaagc gaagcgcgcg gcgggcggg
//
