LOCUS       sequence-481551-       17255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17255)
  AUTHORS   Sung YC, Li Y, Bernasconi Z, Baik S, Asuke S, Keller B, Fahima T, 
            Coaker G
  TITLE     Wheat tandem kinase RWT4 directly binds a fungal effector to 
            activate defense.
  JOURNAL   Nat Genet. 2025 Apr 14. doi: 10.1038/s41588-025-02162-w.
  PUBMED    40229601
REFERENCE   2  (bases 1 to 17255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 Apr 14. doi: 10.1038/s41588-025-02162-w."
COMMENT     Sequence Label: pTA7001-RWT4Ca
FEATURES             Location/Qualifiers
     source          1..17255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2803..2868
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(2887..2911)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2919..2984
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      complement(3510..3762)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(4877..5060)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(4913..5092)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(6384..6617)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        complement(6879..7224)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    7739..7763
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8228..8878
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(9066..9083)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9317..9336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            9559..9668
                     /note="incP origin of transfer"
     CDS             9701..10072
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      10199..10910
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  11533..12300
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             12492..13286
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             13585..14733
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    16329..16353
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(16600..16621)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     16812..16831
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16956..16978
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16970..16987
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16971..16987
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        17197..17243
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 agtcgactct agcctcgaga caagtttgta caaaaaagca ggcttaccac catgatgggt
       61 ggctatgagt tccagagggc agaattggac gcccttgagg gtgtagttcg tgacccaacc
      121 gccgaaccca tgtctttaac actacctctt ttgcgtcaca ttacgaatga tttctcccct
      181 gagttcgaga tatctaagga cgactccgcc gtggtatatc taggagtgct accatcagga
      241 tttagggtgg cagtgaagaa atcacatttt aggttttgtc tggacgacga ggacgccttc
      301 actaatgaag tttcaattgc aatgaaagcc gcacataaga atactgtaag agtaatcggc
      361 tattgtcatc atacacatga gcagattgcc gagtatgagg gaaagcaagt cttcgcagaa
      421 gtgcgtgaga ggttgatatg tactgagtac gtgcccaatg gacctctatc cggccatatc
      481 gaaggcaaaa tttgtgcaca aatggacggc tacgagttcc agcgtgctga gcttgatgca
      541 ttggagagag ttgttagaga tacctctgcc gagccaatgt ccttgacact gccccttttg
      601 cgtcatatca caaatgattt ctcagatgaa tccaggatcg gacgtggagg attcgctgtt
      661 gtctatcttg gtgttttgcc atccggcctg agaatagctg ttaagaggtt gtcaaatata
      721 gcttatatga atgaaagtgc attccagaac gaagtattca tcacaatgaa agccacacat
      781 aaaaatacag tgagattcat gggatactgc tcacagattc aaggcaaact aatagaacat
      841 gatggacaac atgtctttgc acagcttgaa gagaggctaa tttgtgtgga atacgcacct
      901 aagggaacat tagacgctca catcggagat tatggtgaac tggactggaa ccaaagatat
      961 cagattctga aaggcatatg tcagggttta catcatctac atgacgaaat gcatgtcttt
     1021 catggagata tcaaacccgc caacattctt atcggcgata acctagttcc taaaatctat
     1081 gatttcggat tgagtcaaat gttcgaggag gaggaaacgg aaagaattgt ggaaaacatt
     1141 gccggtacat tcggctacat ggcacctgag ttctgtacaa acaacatggt atccttcaag
     1201 gccgagattt acagtttagg cgttgtaata ggagaacttc ttatcggtaa aaaaggatgg
     1261 tttgacgagg acgtgcggaa gctttttgtt cagcagttga aaggccttcg caaaactttg
     1321 gtgaaggaag gcgcgttttc cagctgggag aataaatacc atcaagtgcg cacgtgtatg
     1381 gagattggtc aggattgcat cgatccaaat cctcataaac ggcctacgct tctcgaaatt
     1441 attcaacgcc ttaacgaagc cgaagacatg aattatagtg cggcatcact ctggcaggtt
     1501 cagtctggtg atgaagaatc agatctctcc gatactgaag cgttggagac agagaccacg
     1561 agcgagtttc ttccgtccga tgaagaaccg gcgtccgtcg gtaagaccgg cgagacctct
     1621 acacaggaac cggacaaacc cgatcttata tctaaacttc ccgcttcggt tgacctttct
     1681 gatctcaagg tgcttgaaaa gataaccgat gattttagcc atgagagaat agtcgggaaa
     1741 gacgggactt ttaaaggatg ccataaggct ttcgtttata aaggtgacat tcctctgagg
     1801 gagatgattg cagttaaacg gctcattggt gttgagatac ccttcgagaa gttcaagaga
     1861 gaggctgaac aattcatcag tttggatcat aagaacatag ttaaggttgc aagttattgc
     1921 cacgaccaga gccggggaca tagactcgtc cagttcaagg ggaagcctct gccccaactt
     1981 tttaatgggc ctgagcaact gttgtgttac gagtacatgc acaatggttc tcttagagat
     2041 tacttgatgg gccaaggctc ccgggtcata gattggcaga tgcggtacaa gctgataaaa
     2101 gggacctgcg caggcttgca ttatcttcat aagggtcgcg caggatgtcc gatcgttcac
     2161 cttaatctga gtcccagcaa cgtgcttttg gatcataatt acattccgcg gataactggc
     2221 ttcgactttt ccaagcttat aggagaaaaa aacactaaat ctgtggtctt gaagctcaat
     2281 ggacccatcg cttatctgcc ccccgacttc ttccacagca aaggtacaga tctcaagtac
     2341 ctggcgacag ttgatatata ctctttgggc ctgatgatac tcgagattgc aacacagcag
     2401 gaaatcaagg gcatacatgg tgttctgata aagtcaatcg aggaaaactg gcgggaagag
     2461 tcccaaatca cacgcctcta tacctctctt ggggctgatg agctgaggca ggtgaagatg
     2521 tgtattgata taggacttga ctgtgtcaag agtaacccag agaagcgccc cactgcagga
     2581 gcaattatgt tgtggcttga taaagaatct aagccagtcc ccgtgtcacg ggccggagcg
     2641 ggcgttttgc cacggccccc agttccgacc aacatcaacc atgcggggcg catccagggt
     2701 atcccaatcg agaaatcaca agtccttatt ccttcattcg cttgtaatga atatagctct
     2761 gtcaaaattc aaaatttgca cttgctgctc tttatgggta gcgactacaa ggaccacgac
     2821 ggtgactaca aggaccacga catcgactac aaggacgacg acgacaagat ctagacccag
     2881 ctttctaccc agctttcttg tacaaagtgg tgctcgacga ttataaggat catgatggag
     2941 attataagga tcatgatatt gattataagg atgatgatga taagtaaact agtcgatcca
     3001 ggcctcccag ctttcgtccg tatcatcggt ttcgacaacg ttcgtcaagt tcaatgcatc
     3061 agtttcattg cccacacacc agaatcctac taagtttgag tattatggca ttggaaaagc
     3121 tgttttcttc tatcatttgt tctgcttgta atttactgtg ttctttcagt ttttgttttc
     3181 ggacatcaaa atgcaaatgg atggataaga gttaataaat gatatggtcc ttttgttcat
     3241 tctcaaatta ttattatctg ttgtttttac tttaatgggt tgaatttaag taagaaagga
     3301 actaacagtg tgatattaag gtgcaatgtt agacatataa aacagtcttt cacctctctt
     3361 tggttatgtc ttgaattggt ttgtttcttc acttatctgt gtaatcaagt ttactatgag
     3421 tctatgatca agtaattatg caatcaagtt aagtacagta taggcttttt gtgtcgaggg
     3481 ggtaccgagt cgagggggga tcaattcccg atctagtaac atagatgaca ccgcgcgcga
     3541 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg
     3601 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat
     3661 tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg
     3721 attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgggggatc
     3781 agcttgtgat catgttacat cacctccaag atcccggacg agtgctgggg cgtcggtttc
     3841 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     3901 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     3961 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     4021 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
     4081 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     4141 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     4201 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
     4261 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
     4321 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
     4381 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
     4441 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg
     4501 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
     4561 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg
     4621 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
     4681 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
     4741 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
     4801 tcgcggtgag ttcaggcttt ttcatatctc attgcccccc gggatcttgc aggtcgagat
     4861 cctctagcgg atccggtgca gattatttgg attgagagtg aatatgagac tctaattgga
     4921 taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct
     4981 gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc
     5041 tcattaaact ccagaaaccc gcggctgagt ggctccttca acgttgcggt tctgtcagtt
     5101 ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat
     5161 tctccgctca tgatcagatt gtcgtttccc gccttcagtt tcaagcttgt ttggggatct
     5221 agtgttttac tcctcatatt aacttcggtc attagaggcc acgatttgac acatttttac
     5281 tcaaaacaaa atgtttgcat atctcttata atttcaaatt caacacacaa caaataagag
     5341 aaaaaacaaa taatattaat ttgagaatga acaaaaggac catatcattc attaactctt
     5401 ctccatccat ttccatttca cagttcgata gcgaaaaccg aataaaaaac acagtaaatt
     5461 acaagcacaa caaatggtac aagaaaaaca gttttcccaa tgccataata ctcgaacaag
     5521 ctagcttact cagttaggtc gagtcatttt tgatgaaaca gaagcttttt gatatttcca
     5581 tttgaatatt ttggtatctg attagtgatg atttcagcta acatctctgg gaattcaata
     5641 ctcatggtct tatccaaaaa tgtctggaag cagtaggtaa ggagattctc aaccacctca
     5701 tgcatggagt ccagaagctt tgtcagttgg taaaaccgtt gccagttctg actggagttc
     5761 ccttcccttt tgacgatggc ttttcctagc tctttgatat aagtcattcg aatctcatca
     5821 aataactctt ggctcttcag accttcctta ggaactgagg agagaagcag taaggttttc
     5881 atacagagat actcttcata ggatacctgc aatctttgta attcagagga gacaaacagc
     5941 atgtgtttac attggtcata catgcagggt agagacattc tctgctcatt aataatcaga
     6001 tcaggagcaa agcagagcag gtttccactt gattgtctgt atgatctcca acccagggca
     6061 aatgccatga gaaacatcca tgagtactgt agcagggtca tttggtcatc caggtgtaag
     6121 tttctgaagc ctggtatcgc ctttgcccat ttcactgctg caatcacttg acgcccacct
     6181 aacatgttga gtgtggtcat aattctccat gctgaatctg gaacagagct atcatatcct
     6241 gcatacaaca cctcgggttc aatcacctcc agcagtgaca ccaaggtagg ggtgagctgt
     6301 ggtaatgcag caggaactat tgttttgtta ggattttccg aagtgtcttg tgagactcct
     6361 gcagtggctt gctgaattgg atccccaccg tactcgtcaa ttccaagggc atcggtaaac
     6421 atctgctcaa actcgaagtc ggccatatcc agagcgccgt agggggcgga gtcgtggggg
     6481 gtaaatcccg gacccgggga atccccgtcc cccaacatgt ccagatcgaa atcgtctagc
     6541 gcgtcggcat gcgccatcgc cacgtcctcg ccgtctaagt ggagctcgtc ccccaggctg
     6601 acatcggtcg ggggggcgct cgacctagga aaaatcagta gaaatagctg ttccagtctt
     6661 tctagccttg attccacttc tgtcagatgt gccctagtca gcggagacct tttggttttg
     6721 ggagagtagc gacactccca gttgttcttc agacacttgg cgcacttcgg tttttctttg
     6781 gagcacttga gctttttaag tcggcaaata tcgcatgctt gttcgataga agacagtagc
     6841 ttcattggat ctgccattgt ttaaactaga gtcccccgtg ttctctccaa atgaaatgaa
     6901 cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
     6961 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     7021 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     7081 tcaacgatgg cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttcc
     7141 actatcttca caataaagtg acagatagct gggcaatgga atccgaggag gtttccggat
     7201 attacccttt gttgaaaagt ctcaattgcc ctttggtctt ctgagactgt atctttgata
     7261 tttttggagt agacaagtgt gtcgtgctcc accatgttga cgaagatttt cttcttgtca
     7321 ttgagtcgta agagactctg tatgaactgt tcgccagtct ttacggcgag ttctgttagg
     7381 tcctctattt gaatctttga ctccatggcc tttgattcag tgggaactac ctttttagag
     7441 actccaatct ctattacttg ccttggtttg tgaagcaagc cttgaatcgt ccatactgga
     7501 atagtacttc tgatcttgag aaatatatct ttctctgtgt tcttgatgca gttagtcctg
     7561 aatcttttga ctgcatcttt aaccttcttg ggaaggtatt tgatttcctg gagattattg
     7621 ctcgggtaga tcgtcttgat gagacctgct gcgtaagcct ctctaaccat ctgtgggtta
     7681 gcattctttc tgaaattgaa aaggctaatc tggggacctg caggaaacta tcagtgtttg
     7741 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat
     7801 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg
     7861 gttccccaga tctggcgccg gccagcgaga cgagcaagat tggccgccgc ccgaaacgat
     7921 ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac caacgcatac agcgccagca
     7981 gaatgccata gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg aggcccggca
     8041 gcaccggcat aatcaggccg atgccgacag cgtcgagcgc gacagtgctc agaattacga
     8101 tcaggggtat gttgggtttc acgtctggcc tccggaccag cctccgctgg tccgattgaa
     8161 cgcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt
     8221 gtcaagcatg acaaagttgc agccgaatac agtgatccgt gccgccctgg acctgttgaa
     8281 cgaggtcggc gtagacggtc tgacgacacg caaactggcg gaacggttgg gggttcagca
     8341 gccggcgctt tactggcact tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc
     8401 catgctggcg gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt
     8461 tctgatcggg aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg
     8521 catccatgcc ggcacgcgac cgggcgcacc gcagatggaa acggccgacg cgcagcttcg
     8581 cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga tgacaatcag
     8641 ctacttcact gttggggccg tgcttgagga gcaggccggc gacagcgatg ccggcgagcg
     8701 cggcggcacc gttgaacagg ctccgctctc gccgctgttg cgggccgcga tagacgcctt
     8761 cgacgaagcc ggtccggacg cagcgttcga gcagggactc gcggtgattg tcgatggatt
     8821 ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg acgattgatc
     8881 aggaccgctg ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct
     8941 ccccctttcc accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc
     9001 cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcttcc
     9061 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9421 gcgcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc
     9481 atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg
     9541 tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc
     9601 cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg
     9661 ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc
     9721 aaccaggaag ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat
     9781 tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca
     9841 gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa
     9901 tggcgacctg ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac
     9961 ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga
    10021 cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta
    10081 gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca
    10141 agaagagcga cttcgcggag ctggtgaagt acatcaccga cgagcaaggc aagaccgagc
    10201 gcctttccga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc
    10261 cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt
    10321 tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact
    10381 tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg
    10441 gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc
    10501 ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc
    10561 gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga
    10621 tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc
    10681 aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca
    10741 atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg
    10801 aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc
    10861 ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt
    10921 ccttgccatt gccgggatcg gggcagtaac gggatgggcg atcagcccga gcgcgacgcc
    10981 cggaagcatt gacgtgccgc aggtgctggc atcgacattc agcgaccagg tgccgggcag
    11041 tgagggcggc ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg cattcacgga
    11101 cttcatggcg gggccggcaa tttttacctt gggcattctt ggcatagtgg tcgcgggtgc
    11161 cgtgctcgtg ttcgggggtg cgataaaccc agcgaaccat ttgaggtgat aggtaagatt
    11221 ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat
    11281 ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat
    11341 attgacaata ctgataagat aatatatctt ttatatagaa gatatcgccg tatgtaagga
    11401 tttcaggggg caaggcatag gcagcgcgct tatcaatata tctatagaat gggcaaagca
    11461 taaaaacttg catggactaa tgcttgaaac ccaggacaat aaccttatag cttgtaaatt
    11521 ctatcataat tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc
    11581 gatgactttg tcatgcagct ccaccgattt tgagaacgac agcgacttcc gtcccagccg
    11641 tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct
    11701 gattacgtgc agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca
    11761 tatcaccacg tcaaagggtg acagcaggct cataagacgc cccagcgtcg ccatagtgcg
    11821 ttcaccgaat acgtgcgcaa caaccgtctt ccggagactg tcatacgcgt aaaacagcca
    11881 gcgctggcgc gatttagccc cgacatagcc ccactgttcg tccatttccg cgcagacgat
    11941 gacgtcactg cccggctgta tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga
    12001 cgtaaaatcg tgttgaggcc aacgcccata atgcgggctg ttgcccggca tccaacgcca
    12061 ttcatggcca tatcaatgat tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac
    12121 tgcagttgcc atgttttacg gcagtgagag cagagatagc gctgatgtcc ggcggtgctt
    12181 ttgccgttac gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa
    12241 gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc
    12301 cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac
    12361 aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg
    12421 gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa
    12481 ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
    12541 accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
    12601 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
    12661 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
    12721 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
    12781 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg
    12841 agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag
    12901 acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg
    12961 aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga
    13021 cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct
    13081 ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca
    13141 agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt
    13201 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt
    13261 atattttact ggatgaattg ttttagtacc tagatgtggc gcaacgatgc cggcgacaag
    13321 caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc
    13381 aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac
    13441 gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg
    13501 gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc
    13561 ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa
    13621 gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc
    13681 atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc
    13741 aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc
    13801 gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc
    13861 gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa
    13921 caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa
    13981 atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac
    14041 gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg
    14101 caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag
    14161 ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc
    14221 cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg
    14281 atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg
    14341 atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc
    14401 cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc
    14461 gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg
    14521 tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc
    14581 aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc
    14641 gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg
    14701 gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg
    14761 ggttcagcag ccagcgcttt actggcattt caggaacaag cgggcactgc tcgacgcact
    14821 tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta cgaactgccg ataaacagag
    14881 gattaaaatt gacaattgtg attaaggctc agattcgacg gcttggagcg gccgacgtgc
    14941 aggatttccg cgagatccga ttgtcggccc tgaagaaagc tccagagatg ttcgggtccg
    15001 tttacgagca cgaggagaaa aagcccatgg aggcgttcgc tgaacggttg cgagatgccg
    15061 tggcattcgg cgcctacatc gacggcgaga tcattgggct gtcggtcttc aaacaggagg
    15121 acggccccaa ggacgctcac aaggcgcatc tgtccggcgt tttcgtggag cccgaacagc
    15181 gaggccgagg ggtcgccggt atgctgctgc gggcgttgcc ggcgggttta ttgctcgtga
    15241 tgatcgtccg acagattcca acgggaatct ggtggatgcg catcttcatc ctcggcgcac
    15301 ttaatatttc gctattctgg agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg
    15361 tcgcggcgac ggtaggcgct gtgcagccgc tgatggtcgt gttcatctct gccgctctgc
    15421 taggtagccc gatacgattg atggcggtcc tgggggctat ttgcggaact gcgggcgtgg
    15481 cgctgttggt gttgacacca aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg
    15541 cgggggcggt ttccatggcg ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc
    15601 ctctgctcac ctttaccgcc tggcaactgg cggccggagg acttctgctc gttccagtag
    15661 ctttagtgtt tgatccgcca atcccgatgc ctacaggaac caatgttctc ggcctggcgt
    15721 ggctcggcct gatcggagcg ggtttaacct acttcctttg gttccggggg atctcgcgac
    15781 tcgaacctac agttgtttcc ttactgggct ttctcagccc cagatctggg gtcgatcagc
    15841 cggggatgca tcaggccgac agtcggaact tcgggtcccc gacctgtacc attcggtgag
    15901 caatggatag gggagttgat atcgtcaacg ttcacttcta aagaaatagc gccactcagc
    15961 ttcctcagcg gctttatcca gcgatttcct attatgtcgg catagttctc aagatcgaca
    16021 gcctgtcacg gttaagcgag aaatgaataa gaaggctgat aattcggatc tctgcgaggg
    16081 agatgatatt tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
    16141 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
    16201 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
    16261 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
    16321 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
    16381 acattgcgga cgtttttaat gtactggggt ggtttttctt ttcaccagtg agacgggcaa
    16441 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
    16501 ttgccccagc aggcgaaaat cctgtttgat ggtggttccg aaatcggcaa aatcccttat
    16561 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    16621 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    16681 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
    16741 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    16801 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcca ttcaggctgc gcaactgttg
    16861 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    16921 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    16981 ggccagtgaa ttcctcgaag cttgcatgcc ggtcgactct agaggatccg ggtgacagcc
    17041 ctccgacggg tgacagccct ccgacgggtg acagccctcc gaattctaga ggatccgggt
    17101 gacagccctc cgacgggtga cagccctccg acgggtgaca gccctccgaa ttcgagctcg
    17161 gtacccgggg atctgtcgac ctcgatcgag atcttcgcaa gacccttcct ctatataagg
    17221 aagttcattt catttggaga ggacacgctg aagct
//
