LOCUS       sequence-481569-        9810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express AAV2 transgene (EGFP) and PLRS/tRNA for ncAA incorporation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9810)
  TITLE     An expanded toolbox for precise chemical editing of the 
            adeno-associated virus capsid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIDTsmart-PLRS-4xLtR-ITR-GFP
FEATURES             Location/Qualifiers
     source          1..9810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..22
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   210..350
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   221..350
                     /label=AAV2 ITR
     polyA_signal    390..866
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     648..667
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     CDS             complement(join(925..1635,1636..1638,1639..1641))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(925..1635,1636..1638,1639..1641))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(967..988)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1316..1335
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1575..1596
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     intron          1722..2100
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     primer_bind     complement(2228..2252)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(2249..2452)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2453..2756
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   2832..2961
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        3002..3250
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        3364..3612
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        3726..3974
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        4088..4336
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     enhancer        4489..4792
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4793..4996
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5041..5060
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5041..5059
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5098..5107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5104..7695
                     /codon_start=1
                     /product="aminoacyl-tRNA synthetase specific for the 
                     fluorescent amino acid Anap (Chatterjee et al., 2013)"
                     /label=AnapRS
                     /note="evolved from an E. coli leucyl-tRNA synthetase"
                     /translation="MEEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSILPYPS
                     GRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDN
                     IAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQ
                     TVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRN
                     WIGRSEGVEITFNVNDYDNTLTVYTTRPDAFMGCTYLAVAAGHPLAQKAAENNPELAAF
                     IDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVPG
                     HDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAI
                     ADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDV
                     VMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWIYARYTCPQYKEGMLDSEA
                     ANYWLPVDIAIGGIEHAIMGLLYFRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYY
                     VGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVE
                     RYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAALNVDAL
                     TENQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAPTDGEQDRALMQEA
                     LLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVEDSTLVVVQVNGKVRA
                     KITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVGGPV"
     primer_bind     complement(7733..7750)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7739..7963
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8111..8127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8111..8127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      8175..8206
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        8207..8309
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             8310..9125
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8383..8402)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      9149..9176
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(9188..9775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9268..9287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatccagt cgcgctgctc
      181 tcgtcgatcc gctagggcgg ccgcaaatac ctgcaggcag ctgcgcgctc gctcgctcac
      241 tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag
      301 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct gcggccgctc
      361 ggtccgcacg tggttaccta caaaatcaga aggacaggga agggagcagt ggttcacgcc
      421 tgtaatccca gcaatttggg aggccaaggt gggtagatca cctgagatta ggagttggag
      481 accagcctgg ccaatatggt gaaaccccgt ctctaccaaa aaaacaaaaa ttagctgagc
      541 ctggtcatgc atgcctggaa tcccaacaac tcgggaggct gaggcaggag aatcgcttga
      601 acccaggagg cggagattgc agtgagccaa gattgtgcca ctgcactcca gcttggttcc
      661 caatagaccc cgcaggccct acaggttgtc ttcccaactt gccccttgct ccataccacc
      721 cccctccacc ccataatatt atagaaggac acctagtcag acaaaatgat gcaacttaat
      781 tttattagga caaggctggt gggcactgga gtggcaactt ccagggccag gagaggcact
      841 ggggaggggt cacagggatg ccacccgtag atctctcgag cagcgctgct cgaggcaagc
      901 ttacttgagc tcgagatctg agtacttgta cagctcgtcc atgccgagag tgatcccggc
      961 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
     1021 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
     1081 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
     1141 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
     1201 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
     1261 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
     1321 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
     1381 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
     1441 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
     1501 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
     1561 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
     1621 ctcctcgccc ttgctcacca ttcagaattc aatcgatgtt cgaatcccaa ttctttgcca
     1681 aagtgatggg ccagcacaca gaccagcacg ttgcccagga gctgtgggag gaagataaga
     1741 ggtatgaaca tgattagcaa aagggcctag cttggactca gaataatcca gccttatccc
     1801 aaccataaaa taaaagcaga atggtagctg gattgtagct gctattagca atatgaaacc
     1861 tcttacatca gttacaattt atatgcagaa atatttatat gcagaaatat tgctattgcc
     1921 ttaacccaga aattatcact gttattcttt agaatggtgc aaagaggcat gatacattgt
     1981 atcattattg ccctgaaaga aagagattag ggaaagtatt agaaataaga taaacaaaaa
     2041 agtatattaa aagaagaaag cattttttgt gggcctatag actctatagg cggtacttac
     2101 gtcactcttg gcacggggaa tccgcgttcc aatgcaccgt tcccggccgg gattcgaatc
     2161 cgcggaggct ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc
     2221 tccaggcgat ctgacggttc actaaacgag ctctgcttat atagacctcc caccgtacac
     2281 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     2341 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     2401 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     2461 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     2521 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     2581 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     2641 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     2701 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     2761 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagacg
     2821 cgtgcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     2881 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggcggcctca gtgagcgagc
     2941 gagcgcgcag ctgcctgcag gctcgatccg ctcgcacccc taggggtacc tcgggcagga
     3001 agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     3061 gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     3121 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     3181 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     3241 acgaaacacc gcccgtatgg tggaatcggt agacacaagg gattctaaat ccctcggcgt
     3301 tcgcgctgtg cgggttcaag tcccgctgcg ggcatttttt gctaggggta cctcgggcag
     3361 gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     3421 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     3481 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     3541 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     3601 ggacgaaaca ccgcccgtat ggtggaatcg gtagacacaa gggattctaa atccctcggc
     3661 gttcgcgctg tgcgggttca agtcccgctg cgggcatttt ttgctagggg tacctcgggc
     3721 aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
     3781 gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
     3841 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
     3901 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     3961 aaggacgaaa caccgcccgt atggtggaat cggtagacac aagggattct aaatccctcg
     4021 gcgttcgcgc tgtgcgggtt caagtcccgc tgcgggcatt ttttgctagg ggtacctcgg
     4081 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     4141 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     4201 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     4261 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     4321 gaaaggacga aacaccgccc gtatggtgga atcggtagac acaagggatt ctaaatccct
     4381 cggcgttcgc gctgtgcggg ttcaagtccc gctgcgggca ttttttgcta gttattaata
     4441 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     4501 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     4561 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     4621 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     4681 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     4741 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     4801 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     4861 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     4921 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     4981 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     5041 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttgccgcc
     5101 accatggaag agcaataccg cccggaagag atagaatcca aagtacagct tcattgggat
     5161 gagaagcgca catttgaagt aaccgaagac gagagcaaag agaagtatta ctgcctgtct
     5221 atcctcccct atccttctgg tcgactacac atgggccacg tacgtaacta caccatcggt
     5281 gacgtgatcg cccgctacca gcgtatgctg ggcaaaaacg tcctgcagcc gatcggctgg
     5341 gacgcgtttg gtctgcctgc ggaaggcgcg gcggtgaaaa acaacaccgc tccggcaccg
     5401 tggacgtacg acaacatcgc gtatatgaaa aaccagctca aaatgctggg ctttggttat
     5461 gactggagcc gcgagctggc aacctgtacg ccggaatact accgttggga acagaaattc
     5521 ttcaccgagc tgtataaaaa aggcctggta tataagaaga cttctgcggt caactggtgt
     5581 ccgaacgacc agaccgtact ggcgaacgaa caagttatcg acggctgctg ctggcgctgc
     5641 gataccaaag ttgaacgtaa agagatcccg cagtggttta tcaaaatcac tgcttacgct
     5701 gacgagctgc tcaacgatct ggataaactg gatcactggc cagacaccgt taaaaccatg
     5761 cagcgtaact ggatcggtcg ttccgaaggc gtggagatca ccttcaacgt taacgactat
     5821 gacaacacgc tgaccgttta cactacccgc ccggacgcgt ttatgggttg tacctacctg
     5881 gcggtagctg cgggtcatcc gctggcgcag aaagcggcgg aaaataatcc tgaactggcg
     5941 gcctttattg acgaatgccg taacaccaaa gttgccgaag ctgaaatggc gacgatggag
     6001 aaaaaaggcg tcgatactgg ctttaaagcg gttcacccat taacgggcga agaaattccc
     6061 gtttgggcag caaacttcgt attgatggag tacggcacgg gcgcagttat ggcggtaccg
     6121 gggcacgacc agcgcgacta cgagtttgcc tctaaatacg gcctgaacat caaaccggtt
     6181 atcctggcag ctgacggctc tgagccagat ctttctcagc aagccctgac tgaaaaaggc
     6241 gtgctgttca actctggcga gttcaacggt cttgaccatg aagcggcctt caacgccatc
     6301 gccgataaac tgactgcgat gggcgttggc gagcgtaaag tgaactaccg cctgcgcgac
     6361 tggggtgttt cccgtcagcg ttactggggc gcgccgattc cgatggtgac tctagaagac
     6421 ggtaccgtaa tgccgacccc ggacgaccag ctgccggtga tcctgccgga agatgtggta
     6481 atggacggca ttaccagccc gattaaagca gatccggagt gggcgaaaac taccgttaac
     6541 ggtatgccag cactgcgtga aaccgacact ttcgacacct ttatggagtc ctcctggatc
     6601 tatgcgcgct acacttgccc gcagtacaaa gaaggtatgc tggattccga agcggctaac
     6661 tactggctgc cggtggatat cgctattggt ggtattgaac acgccattat gggtctgctc
     6721 tacttccgct tcttccacaa actgatgcgt gatgcaggca tggtgaactc tgacgaacca
     6781 gcgaaacagt tgctgtgtca gggtatggtg ctggcagatg ccttctacta tgttggcgaa
     6841 aacggcgaac gtaactgggt ttccccggtt gatgctatcg ttgaacgtga cgagaaaggc
     6901 cgtatcgtga aagcgaaaga tgcggcaggc catgaactgg tttataccgg catgagcaaa
     6961 atgtccaagt cgaagaacaa cggtatcgac ccgcaggtga tggttgaacg ttacggcgcg
     7021 gacaccgttc gtctgtttat gatgtttgct tctccggctg atatgactct cgaatggcag
     7081 gaatccggtg tggaaggggc taaccgcttc ctgaaacgtg tctggaaact ggtttacgag
     7141 cacacagcaa aaggtgatgt tgcggcactg aacgttgatg cgctgactga aaatcagaaa
     7201 gcgctgcgtc gcgatgtgca taaaacgatc gctaaagtga ccgatgatat cggccgtcgt
     7261 cagaccttca acaccgcaat tgcggcgatt atggagctga tgaacaaact ggcgaaagca
     7321 ccaaccgatg gcgagcagga tcgcgctctg atgcaggaag cactgctggc cgttgtccgt
     7381 atgcttaacc cgttcacccc gcacatctgc ttcacgctgt ggcaggaact gaaaggcgaa
     7441 ggcgatatcg acaacgcgcc gtggccggtt gctgacgaaa aagcgatggt ggaagactcc
     7501 acgctggtcg tggtgcaggt taacggtaaa gtccgtgcca aaatcaccgt tccggtggac
     7561 gcaacggaag aacaggttcg cgaacgtgct ggccaggaac atctggtagc aaaatatctt
     7621 gatggcgtta ctgtacgtaa agtgatttac gtaccaggta aactcctcaa tctggtcgtt
     7681 ggcgggcccg tttaactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     7741 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     7801 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     7861 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     7921 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     7981 accctagggg tgcgagcgga tcgagcagtg tcgatcacta ctggaccgcg agctgtgctg
     8041 cgacccgtga tcttacggca ttatacgtat gatcggtcca cgatcagcta gattatctag
     8101 tcagcttgat gtcatagctg tttcctgagg ctcaatactg accatttaaa tcatacctga
     8161 cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa
     8221 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
     8281 cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct
     8341 tgaagccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     8401 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag
     8461 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     8521 ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     8581 ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc caggtattag
     8641 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     8701 tgcattcgat tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc
     8761 aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag tgattttgat gacgagcgta
     8821 atggctggcc tgttgaacaa gtctggaaag aaatgcataa actcttgcca ttctcaccgg
     8881 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     8941 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     9001 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     9061 atggtattga taatcctgat atgaataaat tgcagtttca cttgatgctc gatgagtttt
     9121 tctaatgagg acctaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc
     9181 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga tgctcaagtc
     9241 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9301 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9361 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9421 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9481 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9541 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9601 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9661 cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9721 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9781 tcctttgatt ttctaccgaa gaaaggccca
//
