LOCUS       sequence-48159-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2209
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             64..129
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             136..156
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggctagcacc
       61 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
      121 gatgacaaga tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg ggtacctatg
      181 gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa gcctaaggtc
      241 aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac tcatgcgcat
      301 attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa ataccaagat
      361 atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg taaacagtgg
      421 tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag ggggcctccg
      481 ctccagctcg acaccgggca gctgctgaag atcgcgaaga gagggggagt aacagcggta
      541 gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacct gaccccagac
      601 caggtagtcg caatcgcgtc acatgacggg ggaaagcaag ccctggaaac cgtgcaaagg
      661 ttgttgccgg tcctttgtca agaccacggc cttacaccgg agcaagtcgt ggccattgca
      721 aataataacg gtggcaaaca ggctcttgag acggttcaga gacttctccc agttctctgt
      781 caagcccacg ggctgactcc cgatcaagtt gtagcgattg cgtccaacgg tggagggaaa
      841 caagcattgg agactgtcca acggctc
//
