LOCUS       sequence-481626-        8200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid 
            with I-SceI-ELT4 counterselectable site for BpiI cloning. 
            L2-P15A-Gm-ISceI-ELT4; GG assembly from pOGG006, pOGG009, pNDGG006, 
            pGQ0023, pOGG014. GmR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Geddes BA, Williamson R, Schumacher J, Ardi A, Levin G, Cervenka E, 
            Huang R, diCenzo G
  TITLE     BEVA2.0: modular assembly of golden gate-compatible vectors with 
            expanded utility for genetic engineering.
  JOURNAL   Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246.
  PUBMED    39919271
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Can J 
            Microbiol."; date: "2025-01-1"; volume: "71:1-13. doi"; pages: " 
            10.1139/cjm-2024-0246"
COMMENT     Sequence Label: pQGG005
FEATURES             Location/Qualifiers
     source          1..8200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1532..1541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      5627..5721
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(5730..5735,5736..5752,5753..5758)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5947..6480
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            6674..6783
                     /note="incP origin of transfer"
     rep_origin      7166..7711
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     7612..7631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7828..7845
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8109..8195)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgcctgcctt gtcttctggt cgactcgacg gagggtaccg cacggtctgc caatccgacg
       61 gaggtttatg aattttccac cttttccaca agctcaacta gtattaacga tgtggattta
      121 gcaaaaaaaa cctgtaaccc taaatgtaaa ataacgggta agcctgccaa ccatgttatg
      181 gcagattaag cgtctttttg aagggcaccg catctttcgc gttgccgtaa atgtatccgt
      241 ttataaggac agcccgaatg acggtctgcg caaaaaaaca cgttcatctc actcgcgatg
      301 ctgcggagca gttactggct gatattgatc gacgccttga tcagttattg cccgtggagg
      361 gagaacggga tgttgtgggt gccgcgatgc gtgaaggtgc gctggcaccg ggaaaacgta
      421 ttcgccccat gttgctgttg ctgaccgccc gcgatctggg ttgcgctgtc agccatgacg
      481 gattactgga tttggcctgt gcggtggaaa tggtccacgc ggcttcgctg atccttgacg
      541 atatgccctg catggacgat gcgaagctgc ggcgcggacg ccctaccatt cattctcatt
      601 acggagagca tgtggcaata ctggcggcgg ttgccttgct gagtaaagcc tttggcgtaa
      661 ttgccgatgc agatggcctc acgccgctgg caaaaaatcg ggcggtttct gaactgtcaa
      721 acgccatcgg catgcaagga ttggttcagg gtcagttcaa ggatctgtct gaaggggata
      781 agccgcgcag cgctgaagct attttgatga cgaatcactt taaaaccagc acgctgtttt
      841 gtgcctccat gcagatggcc tcgattgttg cgaatgcctc cagcgaagcg cgtgattgcc
      901 tgcatcgttt ttcacttgat cttggtcagg catttcaact gctggacgat ttgaccgatg
      961 gcatgaccga caccggtaag gatagcaatc aggacgccgg taaatcgacg ctggtcaatc
     1021 tgttaggccc gagggcggtt gaagaacgtc tgagacaaca tcttcagctt gccagtgagc
     1081 atctctctgc ggcctgccaa cacgggcacg ccactcaaca ttttattcag gcctggtttg
     1141 acaaaaaact cgctgccgtc agttaaggat gctgcatgag cgcacatgcc ctgcccaagg
     1201 cagatctgac cgccaccagc ctgatcgtat cgggcggcat catcgccgct tggctggccc
     1261 tgcatgtgca tgcgctgtgg tttctggacg cagcggcgca tcccatcctg gcgatcgcaa
     1321 atttcctggg gctgacctgg ctgtcggtcg gattgttcat catcgcgcat gacgcgatgc
     1381 acgggtcggt ggtgccgggg cgtccgcgcg ccaatgcggc gatgggccag cttgtcctgt
     1441 ggctgtatgc cggattttcg tggcgcaaga tgatcgtcaa gcacatggcc catcaccgcc
     1501 atgccggaac cgacgacgac cccgatttcg accatggcgg cccggtccgc tggtacgccc
     1561 gcttcatcgg cacctatttc ggctggcgcg aggggctgct tctgcccgtc atcgtgacgg
     1621 tctatgcgct gatccttggg gatcgctgga tgtacgtggt attctggccg ctgccgtcga
     1681 tcctggcgtc gatccagctg ttcgtgttcg gcacctggct gccgcaccgc cccggccacg
     1741 acgcgttccc ggaccgccac aatgcgcggt cgtcgcggat cagcgacccc gtgtcgctgc
     1801 tgacctgttt tcactttggc ggttatcatc acgaacacca cctgcacccg acggtgccgt
     1861 ggtggcgcct gcccagcacc cgcaccaagg gggacaccgc atgagtgact taagtgggag
     1921 cggctatgca accgcattat gatctgattc tcgtgggggc tggactcgcg aatggcctta
     1981 tcgccctgcg acttcagcag cagcaacctg atatgcgtat tttgcttatc gacgccgcac
     2041 cccaggcggg cgggaatcat acgtggtcat ttcaccacga tgatttgact gagagccaac
     2101 atcgttggat agctccgctg gtggttcatc actggcccga ctatcaggta cgctttccca
     2161 cacgccgtcg taagctgaac agcggctact tttgtattac ttctcagcgt ttcgctgagg
     2221 ttttacagcg acagtttggc ccgcacttgt ggatggatac cgcggtcgca gaggttaatg
     2281 cggaatctgt tcggttgaaa aagggtcagg ttatcggtgc ccgcgcggtg attgacgggc
     2341 ggggttatgc ggcaaattca gcactgagcg tgggcttcca ggcgtttatt ggccaggaat
     2401 ggcgattgag ccacccgcat ggtttatcat ctcccattat catggatgcc acggtcgatc
     2461 agcaaaatgg ttatcgcttc gtgtacagcc tgccgctctc gccgaccaga ttgttaattg
     2521 aagatacgca ctatattgat aatgcgacat tagatcctga atgcgcgcgg caaaatattt
     2581 gcgactatgc cgcgcaacag ggttggcagc ttcagacact gctgcgagaa gaacagggcg
     2641 ccttacccat tactctgtcg ggcaatgccg acgcattctg gcagcagcgc cccctggcct
     2701 gtagtggatt acgtgccggt ctgttccatc ctaccaccgg ctattcactg ccgctggcgg
     2761 ttgccgtggc cgaccgcctg agtgcacttg atgtctttac gtcggcctca attcaccatg
     2821 ccattacgca ttttgcccgc gagcgctggc agcagcaggg ctttttccgc atgctgaatc
     2881 gcatgctgtt tttagccgga cccgccgatt cacgctggcg ggttatgcag cgtttttatg
     2941 gtttacctga agatttaatt gcccgttttt atgcgggaaa actcacgctg accgatcggc
     3001 tacgtattct gagcggcaag ccgcctgttc cggtattagc agcattgcaa gccattatga
     3061 cgactcatcg ttaaagagcg actacatgaa accaactacg gtaattggtg caggcttcgg
     3121 tggcctggca ctggcaattc gtctacaagc tgcggggatc cccgtcttac tgcttgaaca
     3181 acgtgataaa cccggcggtc gggcttatgt ctacgaggat caggggttta cctttgatgc
     3241 aggcccgacg gttatcaccg atcccagtgc cattgaagaa ctgtttgcac tggcaggaaa
     3301 acagttaaaa gagtatgtcg aactgctgcc ggttacgccg ttttaccgcc tgtgttggga
     3361 gtcagggaag gtctttaatt acgataacga tcaaacccgg ctcgaagcgc agattcagca
     3421 gtttaatccc cgcgatgtcg aaggttatcg tcagtttctg gactattcac gcgcggtgtt
     3481 taaagaaggc tatctaaagc tcggtactgt ccctttttta tcgttcagag acatgcttcg
     3541 cgccgcacct caactggcga aactgcaggc atggagaagc gtttacagta aggttgccag
     3601 ttacatcgaa gatgaacatc tgcgccaggc gttttctttc cactcgctgt tggtgggcgg
     3661 caatcccttc gccacctcat ccatttatac gttgatacac gcgctggagc gtgagtgggg
     3721 cgtctggttt ccgcgtggcg gcaccggcgc attagttcag gggatgataa agctgtttca
     3781 ggatctgggt ggcgaagtcg tgttaaacgc cagagtcagc catatggaaa cgacaggaaa
     3841 caagattgaa gccgtgcatt tagaggacgg tcgcaggttc ctgacgcaag ccgtcgcgtc
     3901 aaatgcagat gtggttcata cctatcgcga cctgttaagc cagcaccctg ccgcggttaa
     3961 gcagtccaac aaactgcaga ctaagcgcat gagtaactct ctgtttgtgc tctattttgg
     4021 tttgaatcac catcatgatc agctcgcgca tcacacggtt tgtttcggcc cgcgttaccg
     4081 cgagctgatt gacgaaattt ttaatcatga tggcctcgca gaggacttct cactttatct
     4141 gcacgcgccc tgtgtcacgg attcgtcact ggcgcctgaa ggttgcggca gttactatgt
     4201 gttggcgccg gtgccgcatt taggcaccgc gaacctcgac tggacggttg aggggccaaa
     4261 actacgcgac cgtatttttg cgtaccttga gcagcattac atgcctggct tacggagtca
     4321 gctggtcacg caccggatgt ttacgccgtt tgattttcgc gaccagctta atgcctatca
     4381 tggctcagcc ttttctgtgg agcccgttct tacccagagc gcctggtttc ggccgcataa
     4441 ccgcgataaa accattacta atctctacct ggtcggcgca ggcacgcatc ccggcgcagg
     4501 cattcctggc gtcatcggct cggcaaaagc gacagcaggt ttgatgctgg aggatctgat
     4561 ttgaataatc cgtcgttact caatcatgcg gtcgaaacga tggcagttgg ctcgaaaagt
     4621 tttgcgacag cctcaaagtt atttgatgca aaaacccggc gcagcgtact gatgctctac
     4681 gcctggtgcc gccattgtga cgatgttatt gacgatcaga cgctgggctt tcaggcccgg
     4741 cagcctgcct tacaaacgcc cgaacaacgt ctgatgcaac ttgagatgaa aacgcgccag
     4801 gcctatgcag gatcgcagat gcacgaaccg gcgtttgcgg cttttcagga agtggctatg
     4861 gctcatgata tcgccccggc ttacgcgttt gatcatctgg aaggcttcgc catggatgta
     4921 cgcgaagcgc aatacagcca actggatgat acgctgcgct attgctatca cgttgcaggc
     4981 gttgtcggct tgatgatggc gcaaatcatg ggcgtgcggg ataacgccac gctggaccgc
     5041 gcctgtgacc ttgggctggc atttcagttg accaatattg ctcgcgatat tgtggacgat
     5101 gcgcatgcgg gccgctgtta tctgccggca agctggctgg agcatgaagg tctgaacaaa
     5161 gagaattatg cggcacctga aaaccgtcag gcgctgagcc gtatcgcccg tcgtttggtg
     5221 caggaagcag aaccttacta tttgtctgcc acagccggcc tggcagggtt gcccctgcgt
     5281 tccgcctggg caatcgctac ggcgaagcag gtttaccgga aaataggtgt caaagttgaa
     5341 caggccggtc agcaagcctg ggatcagcgg cagtcaacga ccacgcccga aaaattaacg
     5401 ctgctgctgg ccgcctctgg tcaggccctt acttcccgga tgcgggctca tcctccccgc
     5461 cctgcgcatc tctggcagcg cccgctctag cgccatgtct ttcccggagc gtcgcctgaa
     5521 gttttgacag gggcggcgca tagaggaagc caaaagaaac acaaccttct ttgcccctga
     5581 cggcgtgatg catacgctgg cgattagcac gaagacaagg gacttggact cctgttgata
     5641 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     5701 gcgtttttta ttggtgagaa tccaggcaat tgacataagc ctgttcggtt cgtaaactgt
     5761 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     5821 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagcctatgc
     5881 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     5941 gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     6001 ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga ccaagtcaaa
     6061 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gcgacgtagc cacctactcc
     6121 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac attcatcgcg
     6181 cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt tctgcccaag
     6241 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg agagcaccgg
     6301 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
     6361 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
     6421 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
     6481 caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa attgactact
     6541 tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct
     6601 ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc
     6661 aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct
     6721 tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc
     6781 cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc aagccaacca
     6841 ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga gcgattgagg
     6901 aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc ggccagggct
     6961 acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctggcccgc atcaatggcg
     7021 acctgggccg cctgggcggc ctgctgaaac tctggctcac cgacgacccg cgcacggcgc
     7081 ggttcggtga tgccacgatc ctcgccctgc tggcgaagat cgactctagc tagaaatatt
     7141 ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct
     7201 ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact
     7261 ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt
     7321 agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct
     7381 gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag
     7441 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
     7501 tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac
     7561 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag ggggaaacgc
     7621 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
     7681 atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc tctcacttcc
     7741 ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc
     7801 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc
     7861 ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc acatgaagca
     7921 cttcactgac accctcatca gtgccaacat agtaagccag tatacactcc gctagcgctg
     7981 atgtccggcg gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca
     8041 gttactgacc attccaaggt agggataaca gggtaatttg gaaccgtagt ccagatctag
     8101 ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
     8161 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
//
