LOCUS       sequence-481628-       12812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of 3xFLAG-tagged human LUC7L3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Jourdain AA, Begg BE, Mick E, Shah H, Calvo SE, Skinner OS, Sharma 
            R, Blue SM, Yeo GW, Burge CB, Mootha VK
  TITLE     Loss of LUC7L2 and U1 snRNP subunits shifts energy metabolism from 
            glycolysis to OXPHOS.
  JOURNAL   Mol Cell. 2021 May 6;81(9):1905-1919.e12. doi: 
            10.1016/j.molcel.2021.02.033. Epub 2021 Apr 13.
  PUBMED    33852893
REFERENCE   2  (bases 1 to 12812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.02.033"; journalName: "Mol Cell"; date: 
            "2021-05-6- 6"; volume: "81"; issue: "9"; pages: "1905-1919.e12"
COMMENT     Sequence Label: pWPI-LUC7L3-3xFLAG
FEATURES             Location/Qualifiers
     source          1..12812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    606..731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1224..1457
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1642..1686
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2069..3247
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2300..3238
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3195..3215
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3295..3412
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4755..4820
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4867..5434
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4885..5436
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5029..5046)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5256..5275
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5439..6233
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5493..5512)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6103..6122
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6407..6528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6567..7155
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6620..6640)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7038..7049)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7299..7332)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7352..7532
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7557..7575)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7558..7575)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7865..7884)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8098..8117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8217..8239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8277..8295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8363..8467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8468..8536,8537..9328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8686..8705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9499..10087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9988..10007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10241..10258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10328..10348)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10333..10662
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10513..10648
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11796..11861
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11991..12011
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12436..12570
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             join(12738..12812,1..559)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
       61 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
      121 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
      181 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
      241 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
      301 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
      361 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      421 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      481 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
      541 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
      601 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
      661 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
      721 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
      781 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
      841 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
      901 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
      961 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1021 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     1081 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1141 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1201 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1261 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1321 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1381 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1441 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1501 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     1561 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     1621 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     1681 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     1741 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     1801 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     1861 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     1921 ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgaccatc gatgtcgacg
     1981 ataagctttg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
     2041 cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2101 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     2161 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2221 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2281 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     2341 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     2401 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     2461 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     2521 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     2581 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     2641 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     2701 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     2761 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     2821 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     2881 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     2941 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     3001 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     3061 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     3121 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     3181 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     3241 gtcgtgagga atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa
     3301 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     3361 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca
     3421 cgagactagc ctcgaggttt catatgtaca gaggatccat gatttcggcc gcgcagttgt
     3481 tggatgagtt aatgggccgg gaccgaaacc tagccccgga cgagaagcgc agcaacgtgc
     3541 ggtgggacca cgagagcgtt tgtaaatatt atctctgtgg tttttgtcct gcggaattgt
     3601 tcacaaatac acgttctgat cttggtccgt gtgaaaaaat tcatgatgaa aatctacgaa
     3661 aacagtatga gaagagctct cgtttcatga aagttggcta tgagagagat tttttgcgat
     3721 acttacagag cttacttgca gaagtagaac gtaggatcag acgaggccat gctcgtttgg
     3781 cattatctca aaaccagcag tcttctgggg ccgctggccc aacaggcaaa aatgaagaaa
     3841 aaattcaggt tctaacagac aaaattgatg tacttctgca acagattgaa gaattagggt
     3901 ctgaaggaaa agtagaagaa gcccagggga tgatgaaatt agttgagcaa ttaaaagaag
     3961 agagagaact gctaaggtcc acaacgtcga caattgaaag ctttgctgca caagaaaaac
     4021 aaatggaagt ttgtgaagta tgtggagcct ttttaatagt aggagatgcc cagtcccggg
     4081 tagatgacca tttgatggga aaacaacaca tgggctatgc caaaattaaa gctactgtag
     4141 aagaattaaa agaaaagtta aggaaaagaa ccgaagaacc tgatcgtgat gagcgtctaa
     4201 aaaaggagaa gcaagaaaga gaagaaagag aaaaagaacg ggagagagaa agggaagaaa
     4261 gagaaaggaa aagacgaagg gaagaggaag aaagagaaaa agaaagggct cgtgacagag
     4321 aaagaagaaa gagaagtcgt tcacgaagta gacactcaag ccgaacatca gacagaagat
     4381 gcagcaggtc tcgggaccac aaaaggtcac gaagtagaga aagaaggcgg agcagaagta
     4441 gagatcgacg aagaagcaga agccatgatc gatcagaaag aaaacacaga tctcgaagtc
     4501 gggatcgaag aagatcaaaa agccgggatc gaaagtcata taagcacagg agcaaaagtc
     4561 gggacagaga acaagataga aaatccaagg agaaagaaaa gaggggatct gatgataaaa
     4621 aaagtagtgt gaagtccggt agtcgagaaa agcagagtga agacacaaac actgaatcga
     4681 aggaaagtga tactaagaat gaggtcaatg ggaccagtga agacattaaa tctgaaggtg
     4741 acactcagtc caatgattat aaagatcatg atggcgatta taaagatcat gatattgatt
     4801 ataaagatga tgatgataaa taatagtgag cggcgcacta gtcccgggac tacgggctgc
     4861 aggaattccg cccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     4921 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     4981 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     5041 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     5101 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     5161 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     5221 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     5281 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     5341 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
     5401 ggacgtggtt ttcctttgaa aaacacgatg ataataccat gattgaacaa gatggattgc
     5461 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     5521 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     5581 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     5641 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     5701 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     5761 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5821 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     5881 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5941 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     6001 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     6061 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6121 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6181 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     6241 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     6301 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     6361 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     6421 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6481 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     6541 accgtcgacc tcgagggaat tccgataatc aacctctgga ttacaaaatt tgtgaaagat
     6601 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6661 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6721 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6781 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6841 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6901 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6961 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7021 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7081 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7141 gggccgcctc cccgcatcgg gaattcgagc tcggtacctt taagaccaat gacttacaag
     7201 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7261 tcccaacgaa gacaagatgg gatcaattca ccatgggaat aacttcgtat agcatacatt
     7321 atacgaagtt atgctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7381 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7441 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7501 acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta
     7561 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
     7621 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
     7681 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
     7741 ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc
     7801 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     7861 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     7921 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     7981 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     8041 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac
     8101 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     8161 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8221 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     8281 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     8341 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8401 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8461 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8521 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8581 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8641 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8701 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8761 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8821 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8881 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8941 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9001 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9061 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9121 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9181 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9241 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9301 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9361 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9421 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9601 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9841 cagcttggag cgaacaacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    10141 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10201 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10261 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    10321 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
    10381 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    10441 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
    10501 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10561 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    10621 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca
    10681 ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta
    10741 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc
    10801 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
    10861 catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc
    10921 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
    10981 gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
    11041 tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat
    11101 ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt
    11161 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
    11221 ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat
    11281 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg
    11341 ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga
    11401 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
    11461 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
    11521 ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
    11581 ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga
    11641 tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg
    11701 gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat
    11761 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
    11821 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
    11881 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
    11941 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
    12001 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
    12061 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
    12121 cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca
    12181 gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta
    12241 ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg
    12301 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
    12361 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    12421 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    12481 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    12541 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    12601 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    12661 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    12721 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
    12781 tctgtggatc taccacacac aaggctactt cc
//
