LOCUS       sequence-481901-        5218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To produce AAV9 encoding CAMKII-DrBphP-PCM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Kasatkina LA, Ma C, Sheng H, Lowerison M, Menozzi L, Baloban M, Tang
            Y, Xu Y, Humayun L, Vu T, Song P, Yao J, Verkhusha VV
  TITLE     Deep-tissue high-sensitivity multimodal imaging and optogenetic 
            manipulation enabled by biliverdin reductase knockout.
  JOURNAL   Nat Commun. 2025 Jul 14;16(1):6469. doi: 10.1038/s41467-025-61532-4.
  PUBMED    40659617
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-14"; volume: "16(1):6469. doi"; pages: " 
            10.1038/s41467-025-61532-4"
COMMENT     Sequence Label: pAAV-CAMKII-DrBphP-PCM
FEATURES             Location/Qualifiers
     source          1..5218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      563..572
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2150..2170)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     2352..2371
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2481..2621
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2481..2610
                     /label=AAV2 ITR
     rep_origin      2696..3151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2783..2802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2993..3014
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3168..3187)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3287..3309
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3347..3365)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3433..3537
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3538..3606,3607..4398)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3756..3775)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4569..5157
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5058..5077
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccga cgcccgggcg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca gatgctgcac acttgtggac taagtttgtt
      181 cgcatcccct tctccaaccc cctcagtaca tcaccctggg ggaacagggt ccacttgctc
      241 ctgggcccac acagtcctgc agtattgtgt atataaggcc agggcaaaga ggagcaggtt
      301 ttaaagtgaa aggcaggcag gtgttgggga ggcagttacc ggggcaacgg gaacagggcg
      361 tttcggaggt ggttgccatg gggacctgga tgctgacgaa ggctcgcgag gctgtgagca
      421 gccacagtgc cctgctcaga agccccaagc tcgtcagtca agccggttct ccgtttgcac
      481 tcaggagcac gggcaggcga gtggccccta gttctggggg cagcgtgaaa agacggtatc
      541 gataagcttg attcgagcta gcgccaccat ggcgtctcgg gacccgttgc ccttttttcc
      601 accgctttac cttggtggcc cggaaattac caccgagaac tgcgagcgcg agccgattca
      661 tattcccggc agcatccagc cgcacggcgc cctgctcact gccgacgggc acagcggcga
      721 ggtgctccag atgagcctca acgcggccac ttttctggga caggaaccca cagtgctgcg
      781 cggacagacc ctcgccgcac tgctgcccga gcagtggccc gcgctgcaag cggccctgcc
      841 ccccggctgc cccgacgccc tgcaataccg cgcaacgctg gactggcctg ccgccgggca
      901 cctttcgctg acggtgcacc gggtcggcga gttgctgatt ctggagttcg agccgacgga
      961 ggcctgggac agcaccgggc cgcacgcgct gcgcaacgcg atgttcgcgc tcgaaagtgc
     1021 ccccaacctg cgggcgctgg ccgaggtggc gacccagacg gtccgcgagc tgacgggctt
     1081 tgaccgggtg atgctctaca aatttgcccc cgacgccacc ggcgaagtga ttgccgaggc
     1141 ccgccgtgag gggctgcacg cctttctggg ccaccgtttt cccgcgtcgg acattccggc
     1201 gcaggcccgc gcgctctaca cccggcacct gctgcgcctg accgccgaca cccgcgccgc
     1261 cgccgtgccg ctcgatcccg tcctcaaccc gcagacgaat gcgcccaccc cgctgggcgg
     1321 cgccgtgctg cgcgccacct cgcccatgca catgcagtac ctgcggaaca tgggcgtcgg
     1381 gtcgagcctg tcggtgtcgg tggtggtcgg cggccagctc tggggcctga tcgcctgcca
     1441 ccaccagacg ccctacgtgt tgccgcccga cctgcgaacc acgctcgaat acctgggccg
     1501 cttgctgagc ctgcaagttc aggtcaagga agcggcggac gtggcggcct ttcgccagag
     1561 cctgcgggag caccacgcgc gggtggccct cgcggcggcg cactcgctct cgccgcacga
     1621 caccctcagt gacccggcgc ttgacctgct gggcctgatg cgggccgggg gcctgattct
     1681 gcgtttcgag ggccgctggc agacgttggg tgaagtgccg cctgccccgg cggtggacgc
     1741 gctgctggcg tggctcgaaa cccagccggg cgccctggtc cagaccgacg cgctaggcca
     1801 actgtggccc gccggcgccg atctcgcccc cagcgcagcg ggcctgctcg ccatcagcgt
     1861 gggcgagggc tggtcggagt gcctcgtctg gctgcggccc gaactgcggc tggaggtcgc
     1921 ctggggcggg gccactcctg accaggcgaa agacgacctc gggccgcgcc actcattcga
     1981 cacctacctc gaagaaaaac gcggctacgc cgagccctgg catcccggcg aaatcgagga
     2041 ggcgcaggat ctacgtgaca cattgaccgg ggcgctgtga gaattccgct cgagataatc
     2101 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2161 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     2221 ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg
     2281 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     2341 tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     2401 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     2461 aggtttttta aagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     2521 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     2581 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     2641 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     2701 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2761 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2821 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2881 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     2941 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3001 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
     3061 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3121 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     3181 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3241 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3301 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3361 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3421 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3481 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3541 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3601 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3661 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3721 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3781 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3841 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3901 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3961 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4021 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4081 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4141 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4201 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4261 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4321 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4381 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4441 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4501 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4561 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4621 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4681 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4741 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4801 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4861 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4921 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4981 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5041 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5101 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5161 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
