LOCUS       sequence-481902-       14126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An intermediate vector is used to assemble three gRNAs via Golden 
            Gate cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14126)
  TITLE     Versatile molecular tools enabling customizable DNA methylation in 
            Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p1300-dCas9-HV5
FEATURES             Location/Qualifiers
     source          1..14126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             725..790
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             797..817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(842..865,866..868,869..3355,3356..3358,3359..4942)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             842..4942
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4943..4990
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5234..5250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5234..5250)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5247..5269)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5258..5274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5282..5288,5289..5306,5307..5312))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5327..5348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5539..6215
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     6152..6172
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             6282..7307
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    7348..7522
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7600..7624
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8049..8843
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8930..9518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9419..9438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9672..9689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9704..9844
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9770..9789)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9930..9952
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10188..10382
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10448..11521)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11950..12579)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13880..13904
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     14092..14114
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14106..14123
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14107..14123
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgata cgagacctgg cgactctaga ggtctccact gcccgggata tttcacaaat
       61 tgaacataga ctacagaatt ttagaaaaca aactttctct ctcttatctc acctttatct
      121 tttagagaga aaaagttcga cttccggttg accggaatgt atctttgttt tttttgtttt
      181 gtaacatatt tcgttttccg atttagatcg gatctccttt tccgttttgt cggaccttct
      241 tccggtttat ccggatctaa taatatccat cttagactta gctaagtttg gatctgtttt
      301 ttggttagct cttgtcaatc gcctcatcat cagcaagaag gtgaaatttt tgacaaataa
      361 atcttagaat catgtagtgt ctttggacct tgggaatgat agaaacgatt tgttatagct
      421 actctatgta tcagaccctg accaagatcc aacaatctca taggttttgt gcatatgaaa
      481 ccttcgacta acgagaagtg gtcttttaat gagagagata tctaaaatgt tatcttaaaa
      541 gcccactcaa atctcaaggc ataaggtaga aatgcaaatt tggaaagtgg gctgggcctt
      601 ttgtggtaaa ggcctgtaac ctagcccaat attagcaaaa ccctagacgc gtacattgac
      661 atatataaac ccgcctcctc cttgtttagg gtttctacgt gagagagacg aaacacaaac
      721 catggactat aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga
      781 tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc
      841 cgacaagaag tacagcatcg gcctggcaat cggcaccaac tctgtgggct gggccgtgat
      901 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      961 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
     1021 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
     1081 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     1141 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
     1201 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
     1261 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
     1321 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
     1381 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     1441 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
     1501 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
     1561 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
     1621 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
     1681 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
     1741 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
     1801 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
     1861 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     1921 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
     1981 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
     2041 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
     2101 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
     2161 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
     2221 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
     2281 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
     2341 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     2401 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
     2461 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
     2521 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
     2581 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
     2641 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
     2701 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
     2761 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
     2821 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
     2881 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     2941 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
     3001 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
     3061 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
     3121 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
     3181 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     3241 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
     3301 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggacgccat
     3361 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
     3421 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
     3481 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
     3541 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     3601 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
     3661 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
     3721 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
     3781 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
     3841 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     3901 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
     3961 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
     4021 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
     4081 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
     4141 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
     4201 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
     4261 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
     4321 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
     4381 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
     4441 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
     4501 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
     4561 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
     4621 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     4681 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
     4741 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
     4801 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga
     4861 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
     4921 cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa
     4981 aaagaaaaag taaggatcca gagactctta tcaagaatcc catctcttgc ttgctttttt
     5041 ttgttgcttc cctttgatag ggtttgtttt tcttgtttca gtgactttct atgttaaaag
     5101 ataatgtcag taaaaggatt tggttttcta ttattctgaa tcgattacgg aagattcttg
     5161 cttaattcca atctatacaa gtatcgtgaa ataatgaccg tttatgtggt accgagctcg
     5221 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5281 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5341 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5401 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc tagagcagct
     5461 tgccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc
     5521 agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg
     5581 attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac
     5641 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag
     5701 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
     5761 cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact
     5821 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
     5881 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
     5941 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
     6001 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     6061 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     6121 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     6181 aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc
     6241 tatctctctc gagctttcgc agatccgggg ggcaatgaga tatgaaaaag cctgaactca
     6301 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     6361 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     6421 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     6481 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggagttt agcgagagcc
     6541 tgacctattg catctcccgc cgttcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     6601 aactgcccgc tgttctacaa ccggtcgcgg aggctatgga tgcgatcgct gcggccgatc
     6661 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     6721 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     6781 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga tctgatgctt tgggccgagg
     6841 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     6901 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     6961 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     7021 gctacttcga gcggaggcat ccggagcttg caggatcgcc acgactccgg gcgtatatgc
     7081 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     7141 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     7201 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     7261 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa gaaatagagt agatgccgac
     7321 cgggatctgt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga
     7381 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     7441 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     7501 atccagtact aaaatccaga tcccccgaat taattcggcg ttaattcagt acattaaaaa
     7561 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     7621 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     7681 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     7741 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     7801 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     7861 caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac
     7921 tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag
     7981 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
     8041 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
     8101 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
     8161 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
     8221 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
     8281 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
     8341 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
     8401 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
     8461 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
     8521 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
     8581 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
     8641 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
     8701 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
     8761 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
     8821 ttttactgga tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt
     8881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9061 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9541 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9601 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9661 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     9721 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     9781 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     9841 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     9901 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     9961 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
    10021 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
    10081 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
    10141 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
    10201 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
    10261 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
    10321 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
    10381 caggaaagag tccttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
    10441 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
    10501 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
    10561 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
    10621 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
    10681 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
    10741 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
    10801 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
    10861 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
    10921 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
    10981 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
    11041 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
    11101 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
    11161 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
    11221 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
    11281 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
    11341 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
    11401 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
    11461 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
    11521 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc
    11581 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
    11641 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
    11701 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
    11761 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
    11821 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
    11881 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
    11941 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
    12001 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
    12061 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
    12121 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
    12181 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
    12241 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
    12301 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
    12361 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
    12421 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
    12481 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
    12541 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
    12601 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
    12661 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
    12721 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
    12781 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
    12841 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
    12901 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
    12961 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
    13021 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
    13081 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
    13141 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
    13201 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
    13261 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
    13321 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
    13381 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
    13441 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
    13501 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
    13561 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
    13621 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
    13681 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
    13741 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
    13801 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
    13861 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
    13921 actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat tcgccattca
    13981 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    14041 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    14101 gacgttgtaa aacgacggcc agtgcc
//
