LOCUS       sequence-481919-       17767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a CRISPR/dCas9-based fusion protein designed for targeted 
            DNA methylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17767)
  TITLE     Versatile molecular tools enabling customizable DNA methylation in 
            Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: dCas9-KYP (M6)
FEATURES             Location/Qualifiers
     source          1..17767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1713..1733
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1734..1760,1761..1763,1764..4250,4251..4253,
                     4254..5837)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1734..5837
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5853..5873
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5877..5897
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5946..6641
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     terminator      8594..8846
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8875..8891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8875..8891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8888..8910)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8899..8915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8923..8929,8930..8947,8948..8953))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8968..8989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9180..9856
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9793..9813
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9923..10948
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    10989..11163
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11241..11265
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11690..12484
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12571..13159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13060..13079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13313..13330
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13345..13485
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13411..13430)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13571..13593
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13829..14023
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14089..15162)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15591..16220)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    17521..17545
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     17733..17755
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     17747..17764
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     17748..17764
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga cccggggata tattactcaa cttttgattc
       61 gctatttgct gtgcacctgt ggcgttcatc acatcttttg tgacactgtt tgcactggtc
      121 attgctatta caaaggacct tcctgatgtt gaaggagatc gaaagtaagt aactgcacgc
      181 ataaccattt tctttccgct ctttggctca atccatttga cagtcaaaga caatgtttaa
      241 ccagctccgt ttgatatatt gtctttatgt gtttgttcaa gcatgtttag ttaatcatgc
      301 ctttgattga tcttgaatag gttccaaata tcaaccctgg caacaaaact tggagtgaga
      361 aacattgcat tcctcggttc tggacttctg ctagtaaatt atgtttcagc catatcacta
      421 gctttctaca tgcctcaggt gaattcatct atttccgtct taactatttc ggttaattaa
      481 agcacgaaca ccattactgc atgtagaagc ttgataaact atcgccacca atttattttt
      541 gttgcgatat tgttactttc ctcagtatgc agctttgaaa agaccaaccc tcttatcctt
      601 taacaatgaa caggttttta gaggtagctt gatgattcct gcacatgtga tcttggcttc
      661 aggcttaatt ttccaggtaa agcattatga gatactctta tatctcttac atacttttga
      721 gataatgcac aagaacttca taactatatg ctttagtttc tgcatttgac actgccaaat
      781 tcattaatct ctaatatctt tgttgttgat ctttggtaga catgggtact agaaaaagca
      841 aactacacca aggtaaaata cttttgtaca aacataaact cgttatcacg gaacatcaat
      901 ggagtgtata tctaacggag tgtagaaaca tttgattatt gcaggaagct atctcaggat
      961 attatcggtt tatatggaat ctcttctacg cagagtatct gttattcccc ttcctctagc
     1021 tttcaatttc atggtgagga tatgcagttt tctttgtata tcattcttct tcttctttgt
     1081 agcttggagt caaaatcggt tccttcatgt acatacatca aggatatgtc cttctgaatt
     1141 tttatatctt gcaataaaaa tgcttgtacc aattgaaaca ccagcttttt gagttctatg
     1201 atcactgact tggttctaac caaaaaaaaa aaaaatgttt aatttacata tctaaaagta
     1261 ggtttaggga aacctaaaca gtaaaatatt tgtatattat tcgaatttca ctcatcataa
     1321 aaacttaaat tgcaccataa aattttgttt tactattaat gatgtaattt gtgtaactta
     1381 agataaaaat aatattccgt aagttaaccg gctaaaacca cgtataaacc agggaacctg
     1441 ttaaaccggt tctttactgg ataaagaaat gaaagcccat gtagacagct ccattagagc
     1501 ccaaacccta aatttctcat ctatataaaa ggagtgacat tagggttttt gttcgtcctc
     1561 ttaaagcttc tcgttttctc tgccgtctct ctcattcgcg cgacgcaaac gatcttcagg
     1621 tgatcttctt tctccaaatc ctctctcata actctgattt cgtacttgtg tatttgagct
     1681 cacgctctgt ttctctcacc acagccatgg gcccaaaaaa gaagagaaag gtaatggaca
     1741 agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg
     1801 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
     1861 tcaagaagaa cctgatcggc gccctgctgt tcgacagcgg agaaacagcc gaggccaccc
     1921 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
     1981 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
     2041 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
     2101 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
     2161 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
     2221 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
     2281 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     2341 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
     2401 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg
     2461 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     2521 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     2581 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     2641 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     2701 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     2761 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca
     2821 tcgatggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     2881 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     2941 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     3001 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     3061 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     3121 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     3181 acaagggcgc cagcgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     3241 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtacaacg
     3301 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     3361 agcagaaaaa agccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     3421 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg
     3481 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg
     3541 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc
     3601 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt
     3661 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga
     3721 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc
     3781 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga
     3841 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc
     3901 acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg
     3961 tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa
     4021 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc
     4081 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa
     4141 acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt
     4201 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc
     4261 ctcagagctt tctgaaggac gactccatcg ataacaaagt gctgactcgg agcgacaaga
     4321 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact
     4381 ggcgccagct gctgaatgcc aagctgatta cccagaggaa gttcgacaat ctgaccaagg
     4441 ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg
     4501 aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt
     4561 acgacgagaa cgacaaactg atccgggaag tgaaagtgat caccctgaag tccaagctgg
     4621 tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc
     4681 acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta
     4741 agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg
     4801 ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca
     4861 tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga
     4921 tcgagacaaa cggcgaaaca ggcgagatcg tgtgggataa gggccgggac tttgccaccg
     4981 tgcggaaagt gctgtctatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag
     5041 gcggcttcag caaagagtct atcctgccca agaggaacag cgacaagctg atcgccagaa
     5101 agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg
     5161 tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc
     5221 tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg
     5281 aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc
     5341 tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg
     5401 gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg
     5461 agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca
     5521 aacactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg
     5581 ccgacgctaa tctggacaag gtgctgagcg cctacaacaa gcacagagac aagcctatca
     5641 gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg
     5701 ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc
     5761 tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt
     5821 ctcagctggg aggcgactgt ggcggaggct cgccaaaaaa gaagagaaag gtagacccaa
     5881 agaaaaaacg aaaagtaagc ggaagtgaga ccccaggtac atccgaatca gcaacgcctg
     5941 aaagcagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
     6001 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     6061 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     6121 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
     6181 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     6241 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     6301 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     6361 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     6421 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
     6481 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     6541 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     6601 gatactgcga cctccctagc aaactggggc acaagcttaa tggcggtggc ggagggatgg
     6661 atgctaagtc actaactgcc tggtccgctg gaaaaaggaa acgagctaat gctcctgacc
     6721 aaacagagcg aagatcgagt gttcgggttc agaaagtgag acagaaagcg ttagatgaga
     6781 aggcgcgttt agtacaggag agggttaagc tcctcagtga cagaaagagt gaaatttgtg
     6841 tcgatgacac tgagttacat gagaaagaag aggaaaatgt cgatgggagc cctaaacgaa
     6901 gaagccctcc aaagctaacc gcaatgcaga aaggaaagca gaaattgagt gtttctctga
     6961 atggtaagga cgtgaacttg gaacctcatc tcaaagtgac aaagtgtctg aggttattta
     7021 acaagcaata tctcctctgt gtccaggcta agttgagcag gcctgatttg aagggtgtaa
     7081 ctgagatgat aaaagctaag gcgatattgt acccaagaaa aataatcggt gaccttccag
     7141 gtatagacgt tggacaccgt tttttttcaa gagctgaaat gtgtgctgta ggattccata
     7201 accattggct aaatggcatt gattatatgt caatggaata cgaaaaagag tatagtaact
     7261 acaaattacc gcttgctgtt tctattgtta tgtcgggcca gtacgaggat gatctagaca
     7321 atgcagatac agtgacttac actggacagg gagggcataa cttaactggt aataaacgtc
     7381 agataaagga tcaactttta gaacgaggga atttggcgct aaagcactgc tgcgaatata
     7441 atgtgcctgt cagagtaact cgtggtcaca attgcaaaag tagctatacc aaacgagtat
     7501 acacttatga tggactgtac aaggttgaaa agttctgggc acaaaagggc gtttcaggat
     7561 ttacagtgta taagtaccga ctgaaacgat tggaggggca accagaacta actactgatc
     7621 aggtcaactt tgttgctgga cgcataccaa cgagtacttc agaaattgag ggtttggtat
     7681 gtgaggacat ctccggaggg ctagaattta agggtatccc cgccactaat cgtgttgatg
     7741 attcaccagt ttcaccaaca tctggtttca catacatcaa atctttgatt attgagccta
     7801 atgtcataat tccaaagagt tcaactgggt gtaactgccg aggcagctgc actgactcaa
     7861 agaaatgtgc atgtgctaag cttaatgggg gtaactttcc atatgttgac cttaatgatg
     7921 gcagattaat tgagtctcga gatgttgtat ttgaatgtgg tcctcactgt gggtgtgggc
     7981 caaaatgtgt caaccgaact tctcagaagc gtctaagatt caatcttgag gttttccgct
     8041 ctgcaaagaa gggttgggca gttagatcat gggagtacat accagctggt tcaccagtat
     8101 gtgagtacat aggagttgtc aggagaactg ctgatgtgga tactatctct gacaatgaat
     8161 acatatttga gattgactgc caacagacaa tgcaaggtct tggtggaaga cagagaagac
     8221 taagagatgt tgctgtacca atgaataatg gagtcagtca gagcagtgaa gatgagaatg
     8281 cgccagagtt ctgcattgat gctggttcaa caggaaactt tgctaggttt ataaatcaca
     8341 gttgtgaacc aaacctattt gttcagtgcg tcctgagttc tcaccaggat ataaggcttg
     8401 cccgtgtggt tcttttcgca gctgacaaca tttccccaat gcaggagctc acttacgact
     8461 atggatatgc gcttgatagc gttcatggac cggatgggaa ggtgaagcag ctcgcttgct
     8521 actgtggagc gctaaattgt aggaaacgcc tttactaagg atcctgattg atcgatagag
     8581 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     8641 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     8701 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     8761 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     8821 cgcggtgtca tctatgttac tagatcgggg taccgagctc gaattcgtaa tcatgtcata
     8881 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8941 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9001 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9061 acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc ttgccaacat ggtggagcac
     9121 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt
     9181 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
     9241 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc
     9301 gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc
     9361 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     9421 gattgatgtg aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
     9481 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
     9541 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
     9601 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
     9661 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     9721 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
     9781 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     9841 ggagaggaca cgctgaaatc accagtctct ctctacaaat ctatctctct cgagctttcg
     9901 cagatccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     9961 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
    10021 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
    10081 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
    10141 gattccggaa gtgcttgaca ttggggagtt tagcgagagc ctgacctatt gcatctcccg
    10201 ccgttcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctaca
    10261 accggtcgcg gaggctatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
    10321 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
    10381 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
    10441 cgtcgcgcag gctctcgatg atctgatgct ttgggccgag gactgccccg aagtccggca
    10501 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
    10561 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
    10621 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
    10681 tccggagctt gcaggatcgc cacgactccg ggcgtatatg ctccgcattg gtcttgacca
    10741 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
    10801 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
    10861 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
    10921 cagcactcgt ccgagggcaa agaaatagag tagatgccga ccgggatctg tcgatcgaca
    10981 agctcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct
    11041 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
    11101 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
    11161 atcccccgaa ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
    11221 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
    11281 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
    11341 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
    11401 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
    11461 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg
    11521 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
    11581 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
    11641 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
    11701 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
    11761 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
    11821 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
    11881 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
    11941 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
    12001 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
    12061 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
    12121 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
    12181 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
    12241 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
    12301 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
    12361 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
    12421 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
    12481 ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    12541 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    12601 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    12661 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
    12721 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    12781 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    12841 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    12901 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    12961 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    13021 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    13081 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    13141 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    13201 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    13261 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    13321 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
    13381 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
    13441 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
    13501 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
    13561 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
    13621 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
    13681 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
    13741 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
    13801 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
    13861 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
    13921 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
    13981 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
    14041 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
    14101 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
    14161 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
    14221 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
    14281 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
    14341 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
    14401 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
    14461 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
    14521 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
    14581 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
    14641 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
    14701 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
    14761 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
    14821 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
    14881 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
    14941 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
    15001 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
    15061 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
    15121 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
    15181 ggggttccag tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg
    15241 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
    15301 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
    15361 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
    15421 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
    15481 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
    15541 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    15601 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    15661 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    15721 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    15781 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    15841 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    15901 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    15961 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    16021 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    16081 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    16141 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    16201 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    16261 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    16321 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    16381 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    16441 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    16501 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    16561 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    16621 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    16681 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    16741 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    16801 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    16861 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    16921 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    16981 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    17041 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    17101 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    17161 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    17221 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    17281 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    17341 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    17401 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    17461 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
    17521 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
    17581 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
    17641 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    17701 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    17761 cagtgcc
//
