LOCUS       sequence-481925-       17281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a CRISPR/dCas9-based fusion protein designed for targeted 
            DNA methylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17281)
  TITLE     Versatile molecular tools enabling customizable DNA methylation in 
            Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MQ1v-dCas9 (M14)
FEATURES             Location/Qualifiers
     source          1..17281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3119..3139
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3140..3166,3167..3169,3170..5656,5657..5659,
                     5660..7243)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3140..7243
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7259..7279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7283..7303
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7316..7372
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7373..8071
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     terminator      8107..8359
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8388..8404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8388..8404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8401..8423)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8412..8428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8436..8442,8443..8460,8461..8466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8481..8502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8693..9369
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9306..9326
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9436..10461
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    10502..10676
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10754..10778
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11203..11997
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12084..12672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12573..12592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12826..12843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12858..12998
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12924..12943)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13084..13106
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13342..13536
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13602..14675)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15104..15733)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    17034..17058
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     17246..17268
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     17260..17277
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     17261..17277
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagac ccggggatat attactcaac ttttgattcg
       61 ctatttgctg tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca
      121 ttgctattac aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca
      181 taaccatttt ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac
      241 cagctccgtt tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc
      301 tttgattgat cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa
      361 acattgcatt cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag
      421 ctttctacat gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa
      481 gcacgaacac cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg
      541 ttgcgatatt gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt
      601 aacaatgaac aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca
      661 ggcttaattt tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag
      721 ataatgcaca agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt
      781 cattaatctc taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa
      841 actacaccaa ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg
      901 gagtgtatat ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata
      961 ttatcggttt atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct
     1021 ttcaatttca tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta
     1081 gcttggagtc aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt
     1141 ttatatcttg caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga
     1201 tcactgactt ggttctaacc aaaaaaaaaa aaaatgttta atttacatat ctaaaagtag
     1261 gtttagggaa acctaaacag taaaatattt gtatattatt cgaatttcac tcatcataaa
     1321 aacttaaatt gcaccataaa attttgtttt actattaatg atgtaatttg tgtaacttaa
     1381 gataaaaata atattccgta agttaaccgg ctaaaaccac gtataaacca gggaacctgt
     1441 taaaccggtt ctttactgga taaagaaatg aaagcccatg tagacagctc cattagagcc
     1501 caaaccctaa atttctcatc tatataaaag gagtgacatt agggtttttg ttcgtcctct
     1561 taaagcttct cgttttctct gccgtctctc tcattcgcgc gacgcaaacg atcttcaggt
     1621 gatcttcttt ctccaaatcc tctctcataa ctctgatttc gtacttgtgt atttgagctc
     1681 acgctctgtt tctctcacca cagccatggc tagagattct aaggttgaga acaagactaa
     1741 gaaattgaga gttttcgaag ctttcgctgg aattggtgct cagagaaagg ctcttgagaa
     1801 agttagaaaa gatgagtatg aaatcgttgg attggctgaa tggtacgttc ctgctatcgt
     1861 tatgtaccaa gctatccata acaacttcca tactaagctt gagtacaagt ctgtttctag
     1921 agaagagatg atcgattacc ttgaaaacaa gacattgtct tggaactcta agaacccagt
     1981 ttctaacgga tactggaaga gaaagaaaga tgatgagctt aagatcatct acaacgctat
     2041 caagttgtct gagaaggagg gtaacatctt cgatatcaga gatctttaca agagaacttt
     2101 gaagaacatc gatcttttga catactcttt cccttgtcaa gatctttctc agttgggaat
     2161 ccaaaagggt atgaaaagag gatctggtac tagatctgga cttttgtggg aaattgaaag
     2221 agctcttgat tctacagaga agaacgattt gccaaagtac cttttgatgg aaaacgttgg
     2281 tgctcttttg cataagaaaa atgaagagga attgaaccaa tggaagcaga aacttgagtc
     2341 tttgggatac cagaattcta ttgaagttct taacgctgct gattttggtt cttctcaagc
     2401 tagaagaaga gttttcatga tctctactct taatgagttc gttgaattgc ctaagggtga
     2461 taagaagcca aagtctatca agaaagttct taacaagatc gtttctgaga aggatatcct
     2521 taacaacctt ttgaagtaca acttgactga attcaagaaa acaaagtcta acatcaacaa
     2581 ggcttctttg atcggatact ctaaatttaa ctctgagggt tatgtttacg atcctgaatt
     2641 cacaggacca actcttacag cttctggtgc taattctaga atcaagatca aggatggatc
     2701 taacatcaga aagatgaact ctgatgagac ttttctttac atcggattcg attctcagga
     2761 tggaaagaga gttaacgaga tcgaattctt gacagaaaac caaaagatct tcgtttgtgg
     2821 taactctatc tctgttgagg ttcttgaagc tattatcgat aaaatcggag gtccttctgg
     2881 agggccgagc tctggcgcac ccccaccaag tggagggtct cctgccgggt ccccaacatc
     2941 tactgaagaa ggcaccagcg aatccgcaac gcccgagtca ggccctggta catccacaga
     3001 accatctgaa ggtagtgcgc ctggttcccc agctggaagc cctacttcca ccgaagaagg
     3061 cacgtcaacc gaaccaagtg aaggatctgc ccctgggacc agcactgaac catctgagcc
     3121 aaaaaagaag agaaaggtaa tggacaagaa gtacagcatc ggcctggcca tcggcaccaa
     3181 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
     3241 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggcgccc tgctgttcga
     3301 cagcggagaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
     3361 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
     3421 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
     3481 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
     3541 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
     3601 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
     3661 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     3721 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     3781 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     3841 gaatggcctg ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     3901 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga
     3961 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     4021 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     4081 ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct
     4141 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     4201 gagcaagaac ggctacgccg gctacatcga tggcggagcc agccaggaag agttctacaa
     4261 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     4321 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat
     4381 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     4441 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     4501 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     4561 cccctggaac ttcgaggaag tggtggacaa gggcgccagc gcccagagct tcatcgagcg
     4621 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     4681 gtacgagtac ttcaccgtgt acaacgagct gaccaaagtg aaatacgtga ccgagggaat
     4741 gagaaagccc gccttcctga gcggcgagca gaaaaaagcc atcgtggacc tgctgttcaa
     4801 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     4861 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     4921 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     4981 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     5041 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
     5101 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     5161 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     5221 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     5281 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     5341 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     5401 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     5461 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
     5521 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta
     5581 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
     5641 cgactacgat gtggacgcta tcgtgcctca gagctttctg aaggacgact ccatcgataa
     5701 caaagtgctg actcggagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
     5761 ggtcgtgaag aagatgaaga actactggcg ccagctgctg aatgccaagc tgattaccca
     5821 gaggaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
     5881 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
     5941 cctggactcc cggatgaaca ctaagtacga cgagaacgac aaactgatcc gggaagtgaa
     6001 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
     6061 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
     6121 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
     6181 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc
     6241 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa
     6301 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaacaggcg agatcgtgtg
     6361 ggataagggc cgggactttg ccaccgtgcg gaaagtgctg tctatgcccc aagtgaatat
     6421 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag
     6481 gaacagcgac aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt
     6541 cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc
     6601 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt
     6661 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct
     6721 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct
     6781 ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa
     6841 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca
     6901 gaaacagctg tttgtggaac agcacaaaca ctacctggac gagatcatcg agcagatcag
     6961 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaggtgc tgagcgccta
     7021 caacaagcac agagacaagc ctatcagaga gcaggccgag aatatcatcc acctgtttac
     7081 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
     7141 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
     7201 cctgtacgag acacggatcg acctgtctca gctgggaggc gacggtggcg gaggctcgcc
     7261 aaagaaaaaa cgaaaagtag atccgaaaaa gaagaggaag gtgggaggta gtggggctac
     7321 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctagcgagct
     7381 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     7441 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     7501 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     7561 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     7621 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     7681 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     7741 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     7801 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     7861 cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc
     7921 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
     7981 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     8041 ccctagcaaa ctggggcaca agcttaatta aggatcctga ttgatcgata gagctcgaat
     8101 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     8161 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     8221 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     8281 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     8341 tcatctatgt tactagatcg gggtaccgag ctcgaattcg taatcatgtc atagctgttt
     8401 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8461 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8521 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8581 gggagaggcg gtttgcgtat tggctagagc agcttgccaa catggtggag cacgacactc
     8641 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt
     8701 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
     8761 tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag
     8821 gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
     8881 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     8941 gtgaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
     9001 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     9061 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
     9121 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
     9181 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     9241 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     9301 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     9361 acacgctgaa atcaccagtc tctctctaca aatctatctc tctcgagctt tcgcagatcc
     9421 ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     9481 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     9541 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     9601 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     9661 gaagtgcttg acattgggga gtttagcgag agcctgacct attgcatctc ccgccgttca
     9721 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct acaaccggtc
     9781 gcggaggcta tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     9841 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     9901 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     9961 caggctctcg atgatctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
    10021 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
    10081 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
    10141 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
    10201 cttgcaggat cgccacgact ccgggcgtat atgctccgca ttggtcttga ccaactctat
    10261 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
    10321 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
    10381 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
    10441 cgtccgaggg caaagaaata gagtagatgc cgaccgggat ctgtcgatcg acaagctcga
    10501 gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt
    10561 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
    10621 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatccccc
    10681 gaattaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
    10741 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
    10801 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
    10861 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
    10921 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
    10981 attgtaacga tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt
    11041 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
    11101 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
    11161 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
    11221 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
    11281 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
    11341 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
    11401 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
    11461 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
    11521 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
    11581 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
    11641 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
    11701 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
    11761 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
    11821 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
    11881 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
    11941 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
    12001 ctagaatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    12061 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    12121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    12181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    12241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    12301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    12361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    12421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    12481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    12541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    12601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    12661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    12721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    12781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    12841 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
    12901 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
    12961 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
    13021 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
    13081 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
    13141 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
    13201 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
    13261 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
    13321 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
    13381 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
    13441 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
    13501 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
    13561 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
    13621 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
    13681 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
    13741 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
    13801 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
    13861 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
    13921 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
    13981 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
    14041 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
    14101 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
    14161 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
    14221 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
    14281 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
    14341 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
    14401 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
    14461 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
    14521 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
    14581 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
    14641 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
    14701 cagtgccatt gcagggccgg caggcaaccc agccgcttac gcctggccaa ccgcccgttc
    14761 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
    14821 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
    14881 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
    14941 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
    15001 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
    15061 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
    15121 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
    15181 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
    15241 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
    15301 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
    15361 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    15421 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    15481 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    15541 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    15601 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    15661 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    15721 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    15781 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    15841 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    15901 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    15961 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    16021 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    16081 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    16141 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    16201 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    16261 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    16321 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    16381 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    16441 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    16501 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    16561 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    16621 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    16681 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    16741 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    16801 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    16861 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    16921 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
    16981 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
    17041 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
    17101 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
    17161 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    17221 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
    17281 a
//
