LOCUS       sequence-481929-       17413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a CRISPR/dCas9-based fusion protein designed for targeted 
            DNA methylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17413)
  TITLE     Versatile molecular tools enabling customizable DNA methylation in 
            Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MQ1v-dCas9-SRDX (M30)
FEATURES             Location/Qualifiers
     source          1..17413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3119..3139
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3140..3166,3167..3169,3170..5656,5657..5659,
                     5660..7243)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3140..7243
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7259..7279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7283..7303
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7352..8047
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     terminator      8239..8491
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8520..8536)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8520..8536)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8533..8555)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8544..8560
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8568..8574,8575..8592,8593..8598))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8613..8634
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8825..9501
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9438..9458
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9568..10593
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    10634..10808
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10886..10910
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11335..12129
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12216..12804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12705..12724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12958..12975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12990..13130
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13056..13075)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13216..13238
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13474..13668
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13734..14807)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15236..15865)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    17166..17190
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     17378..17400
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     17392..17409
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     17393..17409
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagac ccggggatat attactcaac ttttgattcg
       61 ctatttgctg tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca
      121 ttgctattac aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca
      181 taaccatttt ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac
      241 cagctccgtt tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc
      301 tttgattgat cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa
      361 acattgcatt cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag
      421 ctttctacat gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa
      481 gcacgaacac cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg
      541 ttgcgatatt gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt
      601 aacaatgaac aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca
      661 ggcttaattt tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag
      721 ataatgcaca agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt
      781 cattaatctc taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa
      841 actacaccaa ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg
      901 gagtgtatat ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata
      961 ttatcggttt atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct
     1021 ttcaatttca tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta
     1081 gcttggagtc aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt
     1141 ttatatcttg caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga
     1201 tcactgactt ggttctaacc aaaaaaaaaa aaaatgttta atttacatat ctaaaagtag
     1261 gtttagggaa acctaaacag taaaatattt gtatattatt cgaatttcac tcatcataaa
     1321 aacttaaatt gcaccataaa attttgtttt actattaatg atgtaatttg tgtaacttaa
     1381 gataaaaata atattccgta agttaaccgg ctaaaaccac gtataaacca gggaacctgt
     1441 taaaccggtt ctttactgga taaagaaatg aaagcccatg tagacagctc cattagagcc
     1501 caaaccctaa atttctcatc tatataaaag gagtgacatt agggtttttg ttcgtcctct
     1561 taaagcttct cgttttctct gccgtctctc tcattcgcgc gacgcaaacg atcttcaggt
     1621 gatcttcttt ctccaaatcc tctctcataa ctctgatttc gtacttgtgt atttgagctc
     1681 acgctctgtt tctctcacca cagccatggc tagagattct aaggttgaga acaagactaa
     1741 gaaattgaga gttttcgaag ctttcgctgg aattggtgct cagagaaagg ctcttgagaa
     1801 agttagaaaa gatgagtatg aaatcgttgg attggctgaa tggtacgttc ctgctatcgt
     1861 tatgtaccaa gctatccata acaacttcca tactaagctt gagtacaagt ctgtttctag
     1921 agaagagatg atcgattacc ttgaaaacaa gacattgtct tggaactcta agaacccagt
     1981 ttctaacgga tactggaaga gaaagaaaga tgatgagctt aagatcatct acaacgctat
     2041 caagttgtct gagaaggagg gtaacatctt cgatatcaga gatctttaca agagaacttt
     2101 gaagaacatc gatcttttga catactcttt cccttgtcaa gatctttctc agttgggaat
     2161 ccaaaagggt atgaaaagag gatctggtac tagatctgga cttttgtggg aaattgaaag
     2221 agctcttgat tctacagaga agaacgattt gccaaagtac cttttgatgg aaaacgttgg
     2281 tgctcttttg cataagaaaa atgaagagga attgaaccaa tggaagcaga aacttgagtc
     2341 tttgggatac cagaattcta ttgaagttct taacgctgct gattttggtt cttctcaagc
     2401 tagaagaaga gttttcatga tctctactct taatgagttc gttgaattgc ctaagggtga
     2461 taagaagcca aagtctatca agaaagttct taacaagatc gtttctgaga aggatatcct
     2521 taacaacctt ttgaagtaca acttgactga attcaagaaa acaaagtcta acatcaacaa
     2581 ggcttctttg atcggatact ctaaatttaa ctctgagggt tatgtttacg atcctgaatt
     2641 cacaggacca actcttacag cttctggtgc taattctaga atcaagatca aggatggatc
     2701 taacatcaga aagatgaact ctgatgagac ttttctttac atcggattcg attctcagga
     2761 tggaaagaga gttaacgaga tcgaattctt gacagaaaac caaaagatct tcgtttgtgg
     2821 taactctatc tctgttgagg ttcttgaagc tattatcgat aaaatcggag gtccttctgg
     2881 agggccgagc tctggcgcac ccccaccaag tggagggtct cctgccgggt ccccaacatc
     2941 tactgaagaa ggcaccagcg aatccgcaac gcccgagtca ggccctggta catccacaga
     3001 accatctgaa ggtagtgcgc ctggttcccc agctggaagc cctacttcca ccgaagaagg
     3061 cacgtcaacc gaaccaagtg aaggatctgc ccctgggacc agcactgaac catctgagcc
     3121 aaaaaagaag agaaaggtaa tggacaagaa gtacagcatc ggcctggcca tcggcaccaa
     3181 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
     3241 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggcgccc tgctgttcga
     3301 cagcggagaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
     3361 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
     3421 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
     3481 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
     3541 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
     3601 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
     3661 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     3721 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     3781 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     3841 gaatggcctg ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     3901 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga
     3961 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     4021 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     4081 ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct
     4141 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     4201 gagcaagaac ggctacgccg gctacatcga tggcggagcc agccaggaag agttctacaa
     4261 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     4321 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat
     4381 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     4441 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     4501 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     4561 cccctggaac ttcgaggaag tggtggacaa gggcgccagc gcccagagct tcatcgagcg
     4621 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     4681 gtacgagtac ttcaccgtgt acaacgagct gaccaaagtg aaatacgtga ccgagggaat
     4741 gagaaagccc gccttcctga gcggcgagca gaaaaaagcc atcgtggacc tgctgttcaa
     4801 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     4861 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     4921 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     4981 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     5041 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
     5101 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     5161 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     5221 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     5281 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     5341 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     5401 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     5461 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
     5521 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta
     5581 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
     5641 cgactacgat gtggacgcta tcgtgcctca gagctttctg aaggacgact ccatcgataa
     5701 caaagtgctg actcggagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
     5761 ggtcgtgaag aagatgaaga actactggcg ccagctgctg aatgccaagc tgattaccca
     5821 gaggaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
     5881 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
     5941 cctggactcc cggatgaaca ctaagtacga cgagaacgac aaactgatcc gggaagtgaa
     6001 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
     6061 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
     6121 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
     6181 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc
     6241 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa
     6301 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaacaggcg agatcgtgtg
     6361 ggataagggc cgggactttg ccaccgtgcg gaaagtgctg tctatgcccc aagtgaatat
     6421 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag
     6481 gaacagcgac aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt
     6541 cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc
     6601 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt
     6661 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct
     6721 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct
     6781 ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa
     6841 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca
     6901 gaaacagctg tttgtggaac agcacaaaca ctacctggac gagatcatcg agcagatcag
     6961 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaggtgc tgagcgccta
     7021 caacaagcac agagacaagc ctatcagaga gcaggccgag aatatcatcc acctgtttac
     7081 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
     7141 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
     7201 cctgtacgag acacggatcg acctgtctca gctgggaggc gacggtggcg gaggctcgcc
     7261 aaaaaagaag agaaaggtag acccaaagaa aaaacgaaaa gtaagcggaa gtgagacccc
     7321 aggtacatcc gaatcagcaa cgcctgaaag cagcgagctg attaaggaga acatgcacat
     7381 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     7441 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     7501 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     7561 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     7621 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
     7681 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     7741 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
     7801 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     7861 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     7921 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     7981 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     8041 gcttaatggc ggtggcggag ggatggatgc taagtcacta actgcctggt cccttgatct
     8101 tgacctcgaa ctcagacttg gatttgctct cgatctcgac cttgaactta gactcggatt
     8161 tgctcttgac ctcgatcttg agcttagact cggattcgct taaggatcct gattgatcga
     8221 tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     8281 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     8341 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     8401 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     8461 tcgcgcgcgg tgtcatctat gttactagat cggggtaccg agctcgaatt cgtaatcatg
     8521 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8581 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8641 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8701 ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc aacatggtgg
     8761 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
     8821 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     8881 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
     8941 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
     9001 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     9061 aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
     9121 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
     9181 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
     9241 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
     9301 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     9361 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
     9421 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     9481 catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc tctctcgagc
     9541 tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc
     9601 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     9661 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     9721 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     9781 ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac ctattgcatc
     9841 tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     9901 ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag ccagacgagc
     9961 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
    10021 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
    10081 gcgtccgtcg cgcaggctct cgatgatctg atgctttggg ccgaggactg ccccgaagtc
    10141 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
    10201 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
    10261 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
    10321 aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg cattggtctt
    10381 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
    10441 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
    10501 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
    10561 cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg atctgtcgat
    10621 cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg
    10681 ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
    10741 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa
    10801 tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt
    10861 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
    10921 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
    10981 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
    11041 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
    11101 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa
    11161 aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt
    11221 tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata
    11281 attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct
    11341 aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat
    11401 acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat
    11461 ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac
    11521 atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat
    11581 gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat
    11641 gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt
    11701 cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa
    11761 ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac
    11821 actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag
    11881 gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa
    11941 gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc
    12001 ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt
    12061 gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa
    12121 ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga
    12181 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    12241 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    12301 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    12361 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    12421 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    12481 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    12541 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    12601 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    12661 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    12721 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    12781 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    12841 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    12901 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    12961 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
    13021 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
    13081 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
    13141 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
    13201 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
    13261 gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt
    13321 ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg
    13381 ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt
    13441 tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga
    13501 gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca
    13561 gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc
    13621 cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct
    13681 tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa
    13741 ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc
    13801 tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc
    13861 acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg
    13921 aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg
    13981 ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg
    14041 ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc
    14101 ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc
    14161 accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac
    14221 cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac
    14281 ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc
    14341 cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc
    14401 cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg
    14461 tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc
    14521 atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt
    14581 tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg
    14641 gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc
    14701 tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc
    14761 agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg
    14821 gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc
    14881 aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat
    14941 tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt
    15001 tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt
    15061 accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc
    15121 atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga
    15181 cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa
    15241 tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg
    15301 gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg
    15361 tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc
    15421 cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg
    15481 tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag
    15541 ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt
    15601 ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt
    15661 cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg
    15721 ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg
    15781 cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct
    15841 ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg
    15901 catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt
    15961 tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc
    16021 atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc
    16081 gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc
    16141 gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc
    16201 gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg
    16261 gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca
    16321 agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc
    16381 gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg
    16441 gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg
    16501 gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc
    16561 aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg
    16621 gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg
    16681 tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac
    16741 agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg
    16801 gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt
    16861 ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc
    16921 caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt
    16981 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
    17041 atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga
    17101 acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct
    17161 cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa
    17221 acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct gcgcaactgt
    17281 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    17341 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    17401 acggccagtg cca
//
