LOCUS       sequence-481935-       16672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a CRISPR/dCas9-based fusion protein designed for targeted 
            DNA methylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16672)
  TITLE     Versatile molecular tools enabling customizable DNA methylation in 
            Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MQ1v-dCas9-cSRDX (M40)
FEATURES             Location/Qualifiers
     source          1..16672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3119..3139
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3140..3166,3167..3169,3170..5656,5657..5659,
                     5660..7243)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3140..7243
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7259..7279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7283..7303
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7498..7750
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7779..7795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7779..7795)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7792..7814)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7803..7819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7827..7833,7834..7851,7852..7857))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7872..7893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8084..8760
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     8697..8717
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             8827..9852
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    9893..10067
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10145..10169
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10594..11388
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11475..12063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11964..11983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12217..12234
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12249..12389
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12315..12334)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12475..12497
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12733..12927
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12993..14066)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(14495..15124)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    16425..16449
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     16637..16659
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16651..16668
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16652..16668
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagac ccggggatat attactcaac ttttgattcg
       61 ctatttgctg tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca
      121 ttgctattac aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca
      181 taaccatttt ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac
      241 cagctccgtt tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc
      301 tttgattgat cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa
      361 acattgcatt cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag
      421 ctttctacat gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa
      481 gcacgaacac cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg
      541 ttgcgatatt gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt
      601 aacaatgaac aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca
      661 ggcttaattt tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag
      721 ataatgcaca agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt
      781 cattaatctc taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa
      841 actacaccaa ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg
      901 gagtgtatat ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata
      961 ttatcggttt atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct
     1021 ttcaatttca tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta
     1081 gcttggagtc aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt
     1141 ttatatcttg caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga
     1201 tcactgactt ggttctaacc aaaaaaaaaa aaaatgttta atttacatat ctaaaagtag
     1261 gtttagggaa acctaaacag taaaatattt gtatattatt cgaatttcac tcatcataaa
     1321 aacttaaatt gcaccataaa attttgtttt actattaatg atgtaatttg tgtaacttaa
     1381 gataaaaata atattccgta agttaaccgg ctaaaaccac gtataaacca gggaacctgt
     1441 taaaccggtt ctttactgga taaagaaatg aaagcccatg tagacagctc cattagagcc
     1501 caaaccctaa atttctcatc tatataaaag gagtgacatt agggtttttg ttcgtcctct
     1561 taaagcttct cgttttctct gccgtctctc tcattcgcgc gacgcaaacg atcttcaggt
     1621 gatcttcttt ctccaaatcc tctctcataa ctctgatttc gtacttgtgt atttgagctc
     1681 acgctctgtt tctctcacca cagccatggc tagagattct aaggttgaga acaagactaa
     1741 gaaattgaga gttttcgaag ctttcgctgg aattggtgct cagagaaagg ctcttgagaa
     1801 agttagaaaa gatgagtatg aaatcgttgg attggctgaa tggtacgttc ctgctatcgt
     1861 tatgtaccaa gctatccata acaacttcca tactaagctt gagtacaagt ctgtttctag
     1921 agaagagatg atcgattacc ttgaaaacaa gacattgtct tggaactcta agaacccagt
     1981 ttctaacgga tactggaaga gaaagaaaga tgatgagctt aagatcatct acaacgctat
     2041 caagttgtct gagaaggagg gtaacatctt cgatatcaga gatctttaca agagaacttt
     2101 gaagaacatc gatcttttga catactcttt cccttgtcaa gatctttctc agttgggaat
     2161 ccaaaagggt atgaaaagag gatctggtac tagatctgga cttttgtggg aaattgaaag
     2221 agctcttgat tctacagaga agaacgattt gccaaagtac cttttgatgg aaaacgttgg
     2281 tgctcttttg cataagaaaa atgaagagga attgaaccaa tggaagcaga aacttgagtc
     2341 tttgggatac cagaattcta ttgaagttct taacgctgct gattttggtt cttctcaagc
     2401 tagaagaaga gttttcatga tctctactct taatgagttc gttgaattgc ctaagggtga
     2461 taagaagcca aagtctatca agaaagttct taacaagatc gtttctgaga aggatatcct
     2521 taacaacctt ttgaagtaca acttgactga attcaagaaa acaaagtcta acatcaacaa
     2581 ggcttctttg atcggatact ctaaatttaa ctctgagggt tatgtttacg atcctgaatt
     2641 cacaggacca actcttacag cttctggtgc taattctaga atcaagatca aggatggatc
     2701 taacatcaga aagatgaact ctgatgagac ttttctttac atcggattcg attctcagga
     2761 tggaaagaga gttaacgaga tcgaattctt gacagaaaac caaaagatct tcgtttgtgg
     2821 taactctatc tctgttgagg ttcttgaagc tattatcgat aaaatcggag gtccttctgg
     2881 agggccgagc tctggcgcac ccccaccaag tggagggtct cctgccgggt ccccaacatc
     2941 tactgaagaa ggcaccagcg aatccgcaac gcccgagtca ggccctggta catccacaga
     3001 accatctgaa ggtagtgcgc ctggttcccc agctggaagc cctacttcca ccgaagaagg
     3061 cacgtcaacc gaaccaagtg aaggatctgc ccctgggacc agcactgaac catctgagcc
     3121 aaaaaagaag agaaaggtaa tggacaagaa gtacagcatc ggcctggcca tcggcaccaa
     3181 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
     3241 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggcgccc tgctgttcga
     3301 cagcggagaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
     3361 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
     3421 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
     3481 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
     3541 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
     3601 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
     3661 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     3721 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     3781 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     3841 gaatggcctg ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     3901 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga
     3961 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     4021 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     4081 ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct
     4141 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     4201 gagcaagaac ggctacgccg gctacatcga tggcggagcc agccaggaag agttctacaa
     4261 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     4321 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat
     4381 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     4441 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     4501 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     4561 cccctggaac ttcgaggaag tggtggacaa gggcgccagc gcccagagct tcatcgagcg
     4621 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     4681 gtacgagtac ttcaccgtgt acaacgagct gaccaaagtg aaatacgtga ccgagggaat
     4741 gagaaagccc gccttcctga gcggcgagca gaaaaaagcc atcgtggacc tgctgttcaa
     4801 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     4861 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     4921 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     4981 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     5041 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
     5101 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     5161 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     5221 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     5281 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     5341 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     5401 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     5461 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
     5521 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta
     5581 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
     5641 cgactacgat gtggacgcta tcgtgcctca gagctttctg aaggacgact ccatcgataa
     5701 caaagtgctg actcggagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
     5761 ggtcgtgaag aagatgaaga actactggcg ccagctgctg aatgccaagc tgattaccca
     5821 gaggaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
     5881 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
     5941 cctggactcc cggatgaaca ctaagtacga cgagaacgac aaactgatcc gggaagtgaa
     6001 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
     6061 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
     6121 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
     6181 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc
     6241 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa
     6301 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaacaggcg agatcgtgtg
     6361 ggataagggc cgggactttg ccaccgtgcg gaaagtgctg tctatgcccc aagtgaatat
     6421 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag
     6481 gaacagcgac aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt
     6541 cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc
     6601 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt
     6661 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct
     6721 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct
     6781 ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa
     6841 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca
     6901 gaaacagctg tttgtggaac agcacaaaca ctacctggac gagatcatcg agcagatcag
     6961 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaggtgc tgagcgccta
     7021 caacaagcac agagacaagc ctatcagaga gcaggccgag aatatcatcc acctgtttac
     7081 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
     7141 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
     7201 cctgtacgag acacggatcg acctgtctca gctgggaggc gacggtggcg gaggctcgcc
     7261 aaaaaagaag agaaaggtag acccaaagaa aaaacgaaaa gtaagcggaa gtgagacccc
     7321 aggtacatcc gaatcagcaa cgcctgaaag ccttgatctt gacctcgaac tcagacttgg
     7381 atttgctctc gatctcgacc ttgaacttag actcggattt gctcttgacc tcgatcttga
     7441 gcttagactc ggattcgctt aaggatcctg attgatcgat agagctcgaa tttccccgat
     7501 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     7561 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     7621 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     7681 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     7741 ttactagatc ggggtaccga gctcgaattc gtaatcatgt catagctgtt tcctgtgtga
     7801 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7861 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7921 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7981 ggtttgcgta ttggctagag cagcttgcca acatggtgga gcacgacact ctcgtctact
     8041 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
     8101 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
     8161 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
     8221 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     8281 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgaacatg
     8341 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa
     8401 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc
     8461 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc
     8521 catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa
     8581 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     8641 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     8701 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacacgctga
     8761 aatcaccagt ctctctctac aaatctatct ctctcgagct ttcgcagatc cggggggcaa
     8821 tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     8881 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     8941 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     9001 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     9061 gacattgggg agtttagcga gagcctgacc tattgcatct cccgccgttc acagggtgtc
     9121 acgttgcaag acctgcctga aaccgaactg cccgctgttc tacaaccggt cgcggaggct
     9181 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     9241 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     9301 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     9361 gatgatctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     9421 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     9481 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     9541 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     9601 tcgccacgac tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     9661 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     9721 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     9781 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     9841 gcaaagaaat agagtagatg ccgaccggga tctgtcgatc gacaagctcg agtttctcca
     9901 taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat
     9961 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
    10021 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcccc cgaattaatt
    10081 cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
    10141 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
    10201 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
    10261 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
    10321 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
    10381 atgacagagc gttgctgcct gtgatcaccg cggtttcaaa atcggctccg tcgatactat
    10441 gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta
    10501 gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt
    10561 taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa
    10621 ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct
    10681 aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg
    10741 tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga
    10801 aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc
    10861 atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa
    10921 aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat
    10981 tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac
    11041 gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc
    11101 gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc
    11161 gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt
    11221 gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg
    11281 gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
    11341 gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta cctagaatgc
    11401 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    11461 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    11521 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    11581 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
    11641 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    11701 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    11761 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    11821 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    11881 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    11941 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    12001 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    12061 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    12121 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    12181 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    12241 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
    12301 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
    12361 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
    12421 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
    12481 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
    12541 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
    12601 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
    12661 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
    12721 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
    12781 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
    12841 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
    12901 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
    12961 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
    13021 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
    13081 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
    13141 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
    13201 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
    13261 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
    13321 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
    13381 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
    13441 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
    13501 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
    13561 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
    13621 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
    13681 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
    13741 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
    13801 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
    13861 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
    13921 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
    13981 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
    14041 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
    14101 tgcagggccg gcaggcaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
    14161 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
    14221 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
    14281 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
    14341 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
    14401 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
    14461 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
    14521 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
    14581 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
    14641 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
    14701 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
    14761 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
    14821 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
    14881 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
    14941 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
    15001 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
    15061 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
    15121 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
    15181 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
    15241 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
    15301 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
    15361 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
    15421 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
    15481 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
    15541 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
    15601 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
    15661 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
    15721 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
    15781 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
    15841 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
    15901 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
    15961 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
    16021 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
    16081 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
    16141 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
    16201 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
    16261 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga
    16321 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc
    16381 cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat
    16441 atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg atccaagctc
    16501 aagctgctct agcattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
    16561 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
    16621 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc ca
//
