LOCUS       sequence-481937-       10958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding full length UBR5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Shearer RF, Frikstad KM, McKenna J, McCloy RA, Deng N, Burgess A, 
            Stokke T, Patzke S, Saunders DN
  TITLE     The E3 ubiquitin ligase UBR5 regulates centriolar satellite 
            stability and primary cilia.
  JOURNAL   Mol Biol Cell. 2018 May 9:mbcE17040248. doi: 
            10.1091/mbc.E17-04-0248.
  PUBMED    29742019
REFERENCE   2  (bases 1 to 10958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2018 May 9:mbcE17040248. doi: 10.1091/mbc.E17-04-0248."
COMMENT     Sequence Label: pENTR221 UBR5
FEATURES             Location/Qualifiers
     source          1..10958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(8443..8542)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8559..8578)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8560..8578)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8583..8599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8583..8599)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8701..8720)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8712..9521
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8779..8798)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      9668..10256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10157..10176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10410..10427
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(10586..10613)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(10705..10791)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     10840..10862
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10854..10871
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10855..10871
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(10887..10958,1..28)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgccaactt tgtacaaaaa agcaggcttc cggaccatga cgtccatcca tttcgtggtt
       61 cacccgctgc cgggcaccga ggaccagctc aatgacaggt tacgagaagt ttctgagaag
      121 ctgaacaaat ataatttaaa cagccacccc cctttgaatg tattggaaca ggctactatt
      181 aaacagtgtg tggtgggacc aaatcatgct gcctttcttc ttgaggatgg tagagtttgc
      241 aggattggtt tttcagtaca gccagacaga ttggaattgg gtaaacctga taataatgat
      301 gggtcaaagt tgaacagcaa ctcgggggca gggaggacgt caaggcctgg taggacaagc
      361 gactctccat ggtttctctc aggttctgag actctaggca ggctggcagg caacacctta
      421 ggaagccgct ggagttctgg agtgggtgga agtggtggag gatcctctgg taggtcatca
      481 gctggagctc gagattcccg ccggcagact cgagttattc ggacaggacg ggatcgaggg
      541 tctgggcttt tgggcagtca gccccagcca gttattccag catctgtcat tccagaggag
      601 ctgatttcac aggcccaagt tgttttacaa ggcaaatcca gaagtgtcat tattcgagaa
      661 cttcagagaa caaatcttga tgtgaacctt gctgtaaata atttacttag ccgggatgat
      721 gaagatggag atgatgggga tgatacagcc agcgaatctt atttgcctgg agaggatctt
      781 atgtctctcc ttgatgccga cattcattct gcccacccaa gtgtcattat tgatgcagat
      841 gccatgtttt ctgaagacat tagctatttt ggttaccctt cttttcgtcg ttcatcactt
      901 tccaggctag gctcatctcg agttctcctt cttcccttag agagagactc tgagctgttg
      961 cgtgaacgtg aatccgtttt acgtttacgt gaacgaaggt ggcttgatgg agcctcattt
     1021 gataatgaaa ggggttctac cagcaaggaa ggagagccaa acttggataa gaagaataca
     1081 cctgttcaaa gtccagtatc tctaggagaa gatttgcagt ggtggcctga taaggatgga
     1141 acaaaattca tctgtattgg ggctctgtat tctgaacttc tggctgtcag cagtaaagga
     1201 gaactttatc agtggaaatg gagtgaatct gagccttaca gaaatgccca gaatccttca
     1261 ttacatcatc cacgagcaac atttttgggg ttaaccaatg aaaagatagt cctcctgtct
     1321 gcaaatagca taagagcaac tgtagctaca gaaaataaca aggttgctac atgggtggat
     1381 gaaactttaa gttctgtggc ttctaaatta gagcacactg ctcagactta ctctgaactt
     1441 caaggagagc ggatagtttc tttacattgc tgtgcccttt acacctgcgc tcagctggaa
     1501 aacagtttat attggtgggg tgtagttcct tttagtcaaa ggaggaaaat gttagagaaa
     1561 gctagagcaa aaaataaaaa gcctaaatcc agtgctggta tttcttcaat gccgaacatc
     1621 actgttggta cccaggtatg cttgagaaat aatcctcttt atcatgctgg agcagttgca
     1681 ttttcaatta gtgctgggat tcctaaagtt ggtgtcttaa tggagtcagt ttggaatatg
     1741 aatgacagct gtagatttca acttagatct cctgaaagct tgaaaaacat ggaaaaagct
     1801 agcaaaacta ctgaagctaa gcctgaaagt aagcaggagc cagtgaaaac agaaatgggt
     1861 cctccaccat ctccagcatc cacgtgtagt gatgcatcct caattgccag cagtgcatca
     1921 atgccataca aacgacgacg gtcaacccct gcaccaaaag aagaggaaaa ggtgaatgaa
     1981 gagcagtggt ctcttcggga agtggttttt gtggaagatg tcaagaatgt tcctgttggc
     2041 aaggtgctaa aagtagatgg tgcctatgtt gctgtaaaat ttccaggaac ctccagtaat
     2101 actaactgtc agaacagctc tggtccagat gctgaccctt cttctctcct gcaggattgt
     2161 aggttactta gaattgatga attgcaggtt gtcaaaactg gtggaacacc gaaggttccc
     2221 gactgtttcc aaaggactcc taaaaagctt tgtatacctg aaaaaacaga aatattagca
     2281 gtgaatgtag attccaaagg tgttcatgct gttctgaaga ctggaaattg ggtacgatac
     2341 tgtatctttg atcttgctac aggaaaagca gaacaggaaa ataattttcc tacaagcagc
     2401 attgctttcc ttggtcagaa tgagaggaat gtagccattt tcactgctgg acaggaatct
     2461 cccattattc ttcgagatgg aaatggtacc atctacccaa tggccaaaga ttgcatggga
     2521 ggaataaggg atcccgattg gctggatctt ccacctatta gtagtcttgg aatgggtgtg
     2581 cattctttaa taaatcttcc tgccaattca acaatcaaaa agaaagctgc tgttatcatc
     2641 atggctgtag agaaacaaac cttaatgcaa cacattctgc gctgtgacta tgaggcctgt
     2701 cgacaatatc taatgaatct tgagcaagcg gttgttttag agcagaatct acagatgctg
     2761 cagacattca tcagccacag atgtgatgga aatcgaaata ttttgcatgc ttgtgtatca
     2821 gtttgctttc caaccagcaa taaagaaact aaagaagaag aggaagcgga gcgttctgaa
     2881 agaaatacat ttgcagaaag gctttctgct gttgaggcca ttgcaaatgc aatatcagtt
     2941 gtttcaagta atggcccagg taatcgggct ggatcatcaa gtagccgaag tttgagatta
     3001 cgggaaatga tgagacgttc gttgagagca gctggtttgg gtagacatga agctggagct
     3061 tcatccagtg accaccagga tccagtttca ccccccatag ctccccctag ttgggttcct
     3121 gaccctcctg cgatggatcc tgatggtgac attgatttta tcctggcccc cgctgtggga
     3181 tctcttacca cagcagcaac cggtactggt caaggaccaa gcacctccac tattccaggt
     3241 ccttccacag agccatctgt agtagaatcc aaggatcgaa aggcgaatgc tcattttata
     3301 ttgaaattgt tatgtgacag tgtggttctc cagccctatc tacgagaact tctttctgcc
     3361 aaggatgcaa gagggatgac cccatttatg tcagctgtaa gtggccgagc ttatcctgct
     3421 gcaattacca tcttagaaac tgctcagaaa attgcaaaag ctgaaatatc ctcaagtgaa
     3481 aaagaggaag atgtattcat gggaatggtt tgcccatcag gtaccaaccc tgatgactct
     3541 cctttatatg ttttatgttg taatgacact tgcagtttta catggactgg agcagagcac
     3601 attaaccagg atatttttga gtgtcgaact tgtggcttgc tggagtcact gtgttgttgt
     3661 acggaatgtg caagggtttg tcataaaggt catgattgca aactcaaacg gacatcacca
     3721 acagcctact gtgattgttg ggagaaatgt aaatgtaaaa ctcttattgc tggacagaaa
     3781 tctgctcgtc ttgatctact ttatcgcctg ctcactgcta ctaatctggt tactctgcca
     3841 aacagcaggg gagagcacct cttactattc ttagtacaga cagtcgcaag gcagacggtg
     3901 gagcattgtc aatacaggcc acctcgaatc agggaagatc gtaaccgaaa aacagccagt
     3961 cctgaagatt cagatatgcc agatcatgat ttggagcctc caagatttgc ccagcttgca
     4021 ttggagcgtg ttctacagga ctggaatgcc ttgaaatcta tgattatgtt tgggtcgcag
     4081 gagaataaag accctcttag tgccagcagt agaataggcc atcttttgcc agaagagcaa
     4141 gtatacctca atcagcaaag tggcacaatt cggctggact gtttcactca ttgccttata
     4201 gttaagtgta cagcagatat tttgctttta gatactctac taggtacact agtgaaagaa
     4261 ctccaaaaca aatatacacc tggacgtaga gaagaagcta ttgctgtgac aatgaggttt
     4321 ctacgttcag tggcaagagt ttttgttatt ctgagtgtgg aaatggcttc atccaaaaag
     4381 aaaaacaact ttattccaca gccaattgga aaatgcaagc gtgtattcca agcattgcta
     4441 ccttacgctg tggaagaatt gtgcaacgta gcagagtcac tgattgttcc tgtcagaatg
     4501 gggattgctc gtccaactgc accatttacc ctggctagta ctagcataga tgccatgcag
     4561 ggcagtgaag aattattttc agtggaacca ctaccaccac gaccatcatc tgatcagtct
     4621 agcagctcca gtcagtctca gtcatcctac atcatcagga atccacagca gaggcgcatc
     4681 agccagtcac agcccgttcg gggcagagat gaagaacagg atgatattgt ttcagcagat
     4741 gtggaagagg ttgaggtggt ggagggtgtg gctggagaag aggatcatca tgatgaacag
     4801 gaagaacacg gggaagaaaa tgctgaggca gagggacaac atgatgagca tgatgaagac
     4861 gggagtgata tggagctgga cttgttagca gcagctgaaa cagaaagtga tagtgaaagt
     4921 aaccacagca accaagataa tgctagtggg cgcagaagcg ttgtcactgc agcaactgct
     4981 ggttcagaag caggagcaag cagtgttcct gccttctttt ctgaagatga ttctcaatcg
     5041 aatgactcaa gtgattctga tagcagtagt agtcagagtg acgacataga acaggagacc
     5101 tttatgcttg atgagccatt agaaagaacc acaaatagct cccatgccaa tggtgctgcc
     5161 caagctcccc gttcaatgca gtgggctgtc cgcaacaccc agcatcagcg agcagccagt
     5221 acagcccctt ccagtacatc tacaccagca gcaagttcag cgggtttgat ttatattgat
     5281 ccttcaaact tacgccggag tggtaccatc agtacaagtg ctgcagctgc agcagctgct
     5341 ttggaagcta gcaacgccag cagttaccta acatctgcaa gcagtttagc cagggcttac
     5401 agcattgtca ttagacaaat ctcggacttg atgggcctta ttcctaagta taatcaccta
     5461 gtatactctc agattccagc agctgtgaaa ttgacttacc aagatgcagt aaacttacag
     5521 aactatgtag aagaaaagct tattcccact tggaactgga tggtcagtat tatggattct
     5581 actgaagctc aattacgtta tggttctgca ttagcatctg ctggtgatcc tggacatcca
     5641 aatcatcctc ttcacgcttc tcagaattca gcgagaagag agaggatgac tgcgcgagaa
     5701 gaagctagct tacgaacact tgaaggcaga cgacgtgcca ccttgcttag cgcccgtcaa
     5761 ggaatgatgt ctgcacgagg agacttccta aattatgctc tgtctctaat gcggtctcat
     5821 aatgatgagc attctgatgt tcttccagtt ttggatgttt gctcattgaa gcatgtggca
     5881 tatgtttttc aagcacttat atactggatt aaggcaatga atcagcagac aacattggat
     5941 acacctcaac tagaacgcaa aaggacgcga gaactcttgg aactgggtat tgataatgaa
     6001 gattcagaac atgaaaatga tgatgacacc aatcaaagtg ctactttgaa tgataaggat
     6061 gatgactctc ttcctgcaga aactggccaa aaccatccat ttttccgacg ttcagactcc
     6121 atgacattcc ttgggtgtat acccccaaat ccatttgaag tgcctctggc tgaagccatc
     6181 cccttggctg atcagccaca tctgttgcag ccaaatgcta gaaaggagga tctttttggc
     6241 cgtccaagtc agggtcttta ttcttcatct gccagtagtg ggaaatgttt aatggaggtt
     6301 acagtggata gaaactgcct agaggttctt ccaacaaaaa tgtcttatgc tgccaatctg
     6361 aaaaatgtaa tgaacatgca aaaccggcaa aaaaaagaag gggaagaaca gcccgtgctg
     6421 ccagaagaaa ctgagagttc aaaaccaggg ccatctgctc atgatcttgc tgcacaatta
     6481 aaaagtagct tactagcaga aataggactt actgaaagtg aagggccacc tctcacatct
     6541 ttcaggccac agtgtagctt tatgggaatg gttatttccc atgatatgct gctaggacgt
     6601 tggcgccttt ctttagaact gttcggcagg gtattcatgg aagatgttgg agcagaacct
     6661 ggatcaatcc taactgaatt gggtggtttt gaggtaaaag aatcaaaatt ccgcagagaa
     6721 atggaaaaac tgagaaacca gcagtcaaga gatttgtcac tagaggttga tcgggatcga
     6781 gatcttctca ttcagcagac tatgaggcag cttaacaatc actttggtcg aagatgtgct
     6841 actacaccaa tggctgtaca cagagtaaaa gtcacattta aggatgagcc aggagagggc
     6901 agtggtgtag cacgaagttt ttatacagcc attgcacaag catttttatc aaatgaaaaa
     6961 ttgccaaatc tagagtgtat ccaaaatgcc aacaaaggca cccacacaag tttaatgcag
     7021 agattaagga accgaggaga gagagaccgg gaaagggaga gagaaaggga aatgaggagg
     7081 agtagtggtt tgcgagcagg ttctcggagg gaccgggata gagactttag aagacagctt
     7141 tccatcgaca ctaggccctt tagaccagcc tctgaaggga atcctagcga tgatcctgag
     7201 cctttgccag cacatcggca ggcacttgga gagaggcttt atcctcgtgt acaagcaatg
     7261 caaccagcat ttgcaagtaa aatcactggc atgttgttgg aattatcccc agctcagctg
     7321 cttctccttc tagcaagtga ggattctctg agagcaagag tggatgaggc catggaactc
     7381 attattgcac atggacggga aaatggagct gatagtatcc tggatcttgg attagtagac
     7441 tcctcagaaa aggtacagca ggaaaaccga aagcgccatg gctctagtcg aagtgtagta
     7501 gatatggatt tagatgatac agatgatggt gatgacaatg cccctttgtt ttaccaacct
     7561 gggaaaagag gattttatac tccaaggcct ggcaagaaca cagaagcaag gttgaattgt
     7621 ttcagaaaca ttggcaggat tcttggacta tgtctgttac agaatgaact ctgtcctatc
     7681 acattgaata gacatgtaat taaagtattg cttggtagaa aagtcaattg gcatgatttt
     7741 gctttttttg atcctgtaat gtatgagagt ttgcggcaac taatcctcgc gtctcagagt
     7801 tcagatgctg atgctgtttt ctcagcaatg gatttggcat ttgcaattga cctgtgtaaa
     7861 gaagaaggtg gaggacaggt tgaactcatt cctaatggtg taaatatacc agtcactcca
     7921 cagaatgtat atgagtatgt gcggaaatac gcagaacaca gaatgttggt agttgcagaa
     7981 cagcccttac atgcaatgag gaaaggtcta ctagatgtgc ttccaaaaaa ttcattagaa
     8041 gatttaacgg cagaagattt taggcttttg gtaaatggct gcggtgaagt caatgtgcaa
     8101 atgctgatca gttttacctc tttcaatgat gaatcaggag aaaatgctga gaagcttctg
     8161 cagttcaagc gttggttctg gtcaatagta gagaagatga gcatgacaga acgacaagat
     8221 cttgtttact tttggacatc aagcccatca ctgccagcca gtgaagaagg attccagcct
     8281 atgccctcaa tcacaataag accaccagat gaccaacatc ttcctactgc aaatacttgc
     8341 atttctcgac tttacgtccc actctattcc tctaaacaga ttctcaaaca gaaattgtta
     8401 ctcgccatta agaccaagaa ttttggtttt gtgtagccgc ggacccagct ttcttgtaca
     8461 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     8521 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     8581 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     8641 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     8701 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     8761 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     8821 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     8881 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     8941 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     9001 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     9061 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     9121 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     9181 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     9241 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     9301 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     9361 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     9421 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     9481 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     9541 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     9601 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9661 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9721 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9781 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9841 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9901 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9961 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    10021 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    10081 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10141 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    10201 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10261 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10321 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10381 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10441 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    10501 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
    10561 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
    10621 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
    10681 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
    10741 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
    10801 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
    10861 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
    10921 gttcgttgca acaaattgat gagcaatgct tttttata
//
