LOCUS       sequence-481946-        2709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 1 homology arm for Golden Gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Lariviere PJ, Zuberi Ashraf AHM, Leonard SP, Miller LG, Moran NA, 
            Barrick JE
  TITLE     Single-step genome engineering in the bee gut symbiont Snodgrassella
            alvi
  JOURNAL   bioRxiv 2023.09.19.558440
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.09.19.558440"
COMMENT     Sequence Label: pBTK1058
FEATURES             Location/Qualifiers
     source          1..2709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      707..741
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(1916..2504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1996..2015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2686..2709,1..636)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
       61 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
      121 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
      181 gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
      241 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
      301 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
      361 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ttcagccaat
      421 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
      481 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
      541 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
      601 caacagtact gcgatgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact
      661 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
      721 ttgccgccgg gcgtttttta ttggtccttt ggtcgaaaaa aaaagcccgc actgtcaggt
      781 gtgggctttt ttctgtgttt ccggtctcac gttgctgtgc cgaaatcata tccgacctcc
      841 tgacaaagta acgttatcaa tagcgactgc aaccaatttt attcacttgt ataaacaaca
      901 tgaagcatac acgataaaat tcaagtagcg cccgcagaac taatatttca cccgtaatta
      961 cccaaacagg caattcaggc ctcttctacc aaacactgta aaggaaaacc ggctgcttcc
     1021 gagcgtgaca aaacctgatt acattttgtt tctgcaatat ctttttgaaa cacaccgcat
     1081 atcccgctgc ctgattcatg caccatcagc ataatagcta tggcctgcgt atgtggtaaa
     1141 tgaaacacat ccattaaaat tgcaattaca aagtccattg tcgtgtaatc atcattcagc
     1201 aaaaccactt tatagcgctt tggcggcatt atcatggatt tctggctctg gctaacaatt
     1261 tgtatcttcg aatttgacat tgtctattaa tctacatgta tctgtgggtt tttagtaacg
     1321 gttttaataa ttttatcatt tttcggtaag cttgcttgac gttagttctt aacctatgta
     1381 aaaagactac ttaacaagtc agacatagac tgccagacaa actgttgtac agtcgttcac
     1441 tatttttttg gcctttttgt gtcgtaaact tgttctttat tttgtttttt atgtatagga
     1501 agtttcatgg caactggtat tgttaagtgg tttaacgacg ctaaaggttt tggttttatt
     1561 accccagatg aaggtggcga agatttgttt gcacatttct ctgcaatcaa catgaatggt
     1621 ttcaaaacct tgaaagaagg tcaacgtgta tcttttgatg tgaccaccgg tccaaaaggt
     1681 aaacaagcgg ctaatattca ggaattcagg gtgagaccga ctttcagcca aaaaacttaa
     1741 gaccgccggt cttgtccact accttgcagt aatgcggtgg acaggatcgg cggttttctt
     1801 ttctcttctc aaacgtacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2341 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2461 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2521 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2581 ttgatcgggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     2641 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     2701 actggatat
//
