LOCUS       sequence-482048-       19798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a CRISPR/dCas9-based fusion protein designed for targeted 
            DNA demethylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19798)
  TITLE     Versatile molecular tools enabling customizable DNA methylation in 
            Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TET1cd-dCas9-IBM1cd (D24)
FEATURES             Location/Qualifiers
     source          1..19798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1440..1457)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1440..1456)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1449..1471)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1659..1683
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2984..3613
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4042..5115
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5181..5375
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5611..5633)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5719..5859
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5774..5793
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5874..5891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6045..6633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6125..6144)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6720..7514)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7939..7963
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8041..8215
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8256..9281)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9348..10024)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(9391..9411)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    10215..10236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10251..10256,10257..10274,10275..10281)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10289..10305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10294..10316
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10313..10329
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10313..10329
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(10358..10610)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12203..12898)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(12947..12967)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(12971..12991)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(13007..14590,14591..14593,14594..17080,
                     17081..17083,17084..17110))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(13007..17110)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(17111..17131)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttccctggtt tatacgtggt tttagccggt taacttacgg aatattattt ttatcttaag
       61 ttacacaaat tacatcatta atagtaaaac aaaattttat ggtgcaattt aagtttttat
      121 gatgagtgaa attcgaataa tatacaaata ttttactgtt taggtttccc taaacctact
      181 tttagatatg taaattaaac attttttttt tttttggtta gaaccaagtc agtgatcata
      241 gaactcaaaa agctggtgtt tcaattggta caagcatttt tattgcaaga tataaaaatt
      301 cagaaggaca tatccttgat gtatgtacat gaaggaaccg attttgactc caagctacaa
      361 agaagaagaa gaatgatata caaagaaaac tgcatatcct caccatgaaa ttgaaagcta
      421 gaggaagggg aataacagat actctgcgta gaagagattc catataaacc gataatatcc
      481 tgagatagct tcctgcaata atcaaatgtt tctacactcc gttagatata cactccattg
      541 atgttccgtg ataacgagtt tatgtttgta caaaagtatt ttaccttggt gtagtttgct
      601 ttttctagta cccatgtcta ccaaagatca acaacaaaga tattagagat taatgaattt
      661 ggcagtgtca aatgcagaaa ctaaagcata tagttatgaa gttcttgtgc attatctcaa
      721 aagtatgtaa gagatataag agtatctcat aatgctttac ctggaaaatt aagcctgaag
      781 ccaagatcac atgtgcagga atcatcaagc tacctctaaa aacctgttca ttgttaaagg
      841 ataagagggt tggtcttttc aaagctgcat actgaggaaa gtaacaatat cgcaacaaaa
      901 ataaattggt ggcgatagtt tatcaagctt ctacatgcag taatggtgtt cgtgctttaa
      961 ttaaccgaaa tagttaagac ggaaatagat gaattcacct gaggcatgta gaaagctagt
     1021 gatatggctg aaacataatt tactagcaga agtccagaac cgaggaatgc aatgtttctc
     1081 actccaagtt ttgttgccag ggttgatatt tggaacctat tcaagatcaa tcaaaggcat
     1141 gattaactaa acatgcttga acaaacacat aaagacaata tatcaaacgg agctggttaa
     1201 acattgtctt tgactgtcaa atggattgag ccaaagagcg gaaagaaaat ggttatgcgt
     1261 gcagttactt actttcgatc tccttcaaca tcaggaaggt cctttgtaat agcaatgacc
     1321 agtgcaaaca gtgtcacaaa agatgtgatg aacgccacag gtgcacagca aatagcgaat
     1381 caaaagttga gtaatatatc cccgggtcta gagtcgacct gcaggcatgc aagcttggca
     1441 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1501 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1561 ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag
     1621 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
     1681 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     1741 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
     1801 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     1861 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     1921 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     1981 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     2041 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     2101 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     2161 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     2221 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     2281 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     2341 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     2401 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     2461 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     2521 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     2581 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     2641 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     2701 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     2761 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     2821 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     2881 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     2941 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     3001 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     3061 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     3121 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     3181 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     3241 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     3301 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     3361 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     3421 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     3481 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     3541 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     3601 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     3661 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     3721 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     3781 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     3841 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     3901 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     3961 gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac
     4021 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     4081 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
     4141 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
     4201 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
     4261 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
     4321 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
     4381 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
     4441 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
     4501 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
     4561 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
     4621 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
     4681 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
     4741 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
     4801 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
     4861 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
     4921 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
     4981 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
     5041 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
     5101 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
     5161 ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
     5221 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
     5281 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
     5341 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
     5401 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
     5461 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
     5521 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg
     5581 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     5641 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     5701 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
     5761 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     5821 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     5881 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5941 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6001 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6061 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6121 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6181 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6241 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6301 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6361 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6421 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6481 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6541 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6601 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6661 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac
     6721 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
     6781 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
     6841 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
     6901 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
     6961 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
     7021 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
     7081 taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg
     7141 atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt
     7201 tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
     7261 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
     7321 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
     7381 atttttaaat ataggttttc attttctccc accagcttat ataccttagc aggagacatt
     7441 ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt
     7501 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
     7561 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
     7621 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
     7681 agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     7741 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     7801 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     7861 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     7921 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     7981 acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg
     8041 gatctggatt ttagtactgg attttggttt taggaattag aaattttatt gatagaagta
     8101 ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc
     8161 tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga
     8221 gcttgtcgat cgacagatcc cggtcggcat ctactctatt tctttgccct cggacgagtg
     8281 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
     8341 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
     8401 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
     8461 agttggtcaa gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc
     8521 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
     8581 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg
     8641 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg
     8701 aggtgccgga cttcggggca gtcctcggcc caaagcatca gatcatcgag agcctgcgcg
     8761 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca
     8821 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg
     8881 ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc
     8941 ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgaa
     9001 cggcgggaga tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga
     9061 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg
     9121 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga
     9181 gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga
     9241 aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggat
     9301 ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc
     9361 tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt
     9421 gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt
     9481 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     9541 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
     9601 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     9661 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
     9721 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttcacatca
     9781 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     9841 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     9901 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     9961 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
    10021 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
    10081 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg
    10141 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    10201 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    10261 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    10321 agctatgaca tgattacgaa ttcgagctcg gtaccccgat ctagtaacat agatgacacc
    10381 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
    10441 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
    10501 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
    10561 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc
    10621 gagctctatc gatcaatcag gatccttaca tcttctccat ttctaatctg tcaatgtatg
    10681 cgttggttac ttcacttccc agcttttttc tccgctcata tgtcctcaca atatttggtc
    10741 ttttcacccc ttcctcttcc gtagacttct tttcgcttga agatatgtgt tcgagatcct
    10801 tatccattgc actgactatc ccatttgata tctccacttt ggcttcggaa gacttctctt
    10861 cacttggagg taagtctttg agacccttat cgattgcatt gactatccca tttgatatct
    10921 cctcttcagc tgcttccgaa gacttctttt cacttggaga taagtctttg agagccttgg
    10981 caactgcctt ggctgccccc ttcttcggcc ctctcatatt ttgtttcttc tcttcaggtt
    11041 ctggagactt ctctccactt aagtctctga gagctttatc aactgcatgg actatcatct
    11101 tcttaacccc taacttgtct tcttttgcaa aatgatttgg tggaagtaga cgatactgtt
    11161 ttgtcaagcg taaacactcg ctgacatttt caggtgagac gaagtcaagc gctaccttat
    11221 tgcaggactt caaatttcta acttgatgag ggcaacctac aggtatcaaa acagcatcac
    11281 caagcttctg attgaaggtc caaggttcaa tgccatattc ttctttcagc ttcattatat
    11341 gataccgcgt cagatagaag ttctggtcat gtataggatg aacaacctga gacacaggac
    11401 agcagtagag atgtctgaac tccttgtgat gtttctcaat gtaactttct aatttaggaa
    11461 tatcttctct acggaaaatg tcccaaagag ctccatcatc agtttcaaca ttttggactt
    11521 gttgtctgga attttcaaga atctccatca tttcctcctt gttagctact gagctataca
    11581 gctccttgag atcttgttcg gcatgcttct ttttcagatt tccaattcca ggttgcatat
    11641 tgggcacttc agatatgtgc gtcaaaatat tcaccgcatc agacatgtcg cagtggagct
    11701 tagtaacaga atctccacgg cctaattctt gagcaaatcc agaagcaacg tacgtctttg
    11761 gtcccatgtc tggcttcaag caattttggg gaagcttgac agcgagattc aaaggtccat
    11821 taaccggatg agtgtaatgc ttcaaaggca aactacataa gaactcctca gcatgacgtg
    11881 gcaagttatc tttgaagact ttagctggag gccaatcttt cagtttcaga actagtggcc
    11941 aacccattcg atcatatcgg ccatccgtgt atccagtaaa aaattcatga agattaactt
    12001 ttacctcaca gaagtccaaa caatcaacag caacaacatc cttaagtgat ccatgctgga
    12061 catggcttat ctggcggcag gcacgatgca ttaccattgg ttcccagctt aaaccagatg
    12121 tagcttcaag cacatttctc acaatcacag gctcgccgga ccaggcagtt agtgacttag
    12181 catccatccc tccgccaccg ccattaagct tgtgccccag tttgctaggg aggtcgcagt
    12241 atctggccac tgccacctcg tgctgctcga cgtaggtctc gttgttggcc tccttgattc
    12301 tttccagtct gtagtccaca tagtagacgc caggcatctt gaggttctta gcgggtttct
    12361 tggatctata tgtggtcttg atgtttgcga tcagatggct cccgcccacg agcttcaggg
    12421 ccatgtcgtt tctgccttcc aggccgccgt cagcggggta cagcgtctcg gtgaaggcct
    12481 cccagccgag tgttttcttc tgcatcacag ggccgttgga tgtgaagttc acccctctga
    12541 tcttgacgtt gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca
    12601 cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct
    12661 tgaagaagtc ggggatgccc tgggtgtggt tgatgaaggt cttgctgccg tagaggaagc
    12721 tagtagccag gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca
    12781 tggtctgggt gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtgatggt
    12841 tgtccacggt gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcgctgc
    12901 tttcaggcgt tgctgattcg gatgtacctg gggtctcact tccgcttact tttcgttttt
    12961 tctttgggtc tacctttctc ttcttttttg gcgagcctcc gccaccgtcg cctcccagct
    13021 gagacaggtc gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc agggtggcgt
    13081 ccagcacctc tttggtgctg gtgtacctct tccggtcgat ggtggtgtca aagtacttga
    13141 aggcggcagg ggctcccaga ttggtcaggg taaacaggtg gatgatattc tcggcctgct
    13201 ctctgatagg cttgtctctg tgcttgttgt aggcgctcag caccttgtcc agattagcgt
    13261 cggccaggat cactctcttg gagaactcgc tgatctgctc gatgatctcg tccaggtagt
    13321 gtttgtgctg ttccacaaac agctgtttct gctcattatc ctcgggggag cccttcagct
    13381 tctcatagtg gctggccagg tacaggaagt tcacatattt ggagggcagg gccagttcgt
    13441 ttcccttctg cagttcgccg gcagaggcca gcattctctt ccggccgttt tccagctcga
    13501 acagggagta cttaggcagc ttgatgatca ggtccttttt cacttctttg tagcccttgg
    13561 cttccagaaa gtcgatggga ttcttctcga agctgcttct ttccatgatg gtgatcccca
    13621 gcagctcttt cacactcttc agtttcttgg acttgccctt ttccactttg gccaccacca
    13681 gcacagaata ggccacggtg gggctgtcga agccgccgta cttcttaggg tcccagtcct
    13741 tctttctggc gatcagcttg tcgctgttcc tcttgggcag gatagactct ttgctgaagc
    13801 cgcctgtctg cacctcggtc tttttcacga tattcacttg gggcatagac agcactttcc
    13861 gcacggtggc aaagtcccgg cccttatccc acacgatctc gcctgtttcg ccgtttgtct
    13921 cgatcagagg ccgcttccgg atctcgccgt tggccagggt aatctcggtc ttgaaaaagt
    13981 tcatgatgtt gctgtagaag aagtacttgg cggtagcctt gccgatttcc tgctcgctct
    14041 tggcgatcat cttccgcacg tcgtacacct tgtagtcgcc gtacacgaac tcgctttcca
    14101 gcttagggta ctttttgatc agggcggttc ccacgacggc gttcaggtag gcgtcgtggg
    14161 cgtggtggta gttgttgatc tcgcgcactt tgtaaaactg gaaatccttc cggaaatcgg
    14221 acaccagctt ggacttcagg gtgatcactt tcacttcccg gatcagtttg tcgttctcgt
    14281 cgtacttagt gttcatccgg gagtccagga tctgtgccac gtgctttgtg atctgccggg
    14341 tttccaccag ctgtctcttg atgaagccgg ccttatccag ttcgctcagg ccgcctctct
    14401 cggccttggt cagattgtcg aacttcctct gggtaatcag cttggcattc agcagctggc
    14461 gccagtagtt cttcatcttc ttcacgacct cttcggaggg cacgttgtcg ctcttgcccc
    14521 ggttcttgtc gctccgagtc agcactttgt tatcgatgga gtcgtccttc agaaagctct
    14581 gaggcacgat agcgtccaca tcgtagtcgg acagccggtt gatgtccagt tcctggtcca
    14641 cgtacatatc ccgcccattc tgcaggtagt acaggtacag cttctcgttc tgcagctggg
    14701 tgttttccac ggggtgttct ttcaggatct ggctgcccag ctctttgatg ccctcttcga
    14761 tccgcttcat tctctcgcgg ctgttcttct gtcccttctg ggtggtctgg ttctctctgg
    14821 ccatttcgat cacgatgttc tcgggcttgt gccggcccat cactttcacg agctcgtcca
    14881 ccaccttcac tgtctgcagg atgcccttct taatggcggg gctgccggcc agattggcaa
    14941 tgtgctcgtg caggctatcg ccctggccgg acacctgggc tttctggatg tcctctttaa
    15001 aggtcaggct gtcgtcgtgg atcagctgca tgaagtttct gttggcgaag ccgtcggact
    15061 tcaggaaatc caggattgtc ttgccggact gcttgtcccg gatgccgttg atcagcttcc
    15121 ggctcagcct gccccagccg gtgtatctcc gccgcttcag ctgcttcatc actttgtcgt
    15181 cgaacaggtg ggcataggtt ttcagccgtt cctcgatcat ctctctgtcc tcaaacagtg
    15241 tcagggtcag cacgatatct tccagaatgt cctcgttttc ctcattgtcc aggaagtcct
    15301 tgtccttgat aattttcagc agatcgtggt atgtgcccag ggaggcgttg aaccgatctt
    15361 ccacgccgga gatttccacg gagtcgaagc actcgatttt cttgaagtag tcctctttca
    15421 gctgcttcac ggtcactttc cggttggtct tgaacagcag gtccacgatg gcttttttct
    15481 gctcgccgct caggaaggcg ggctttctca ttccctcggt cacgtatttc actttggtca
    15541 gctcgttgta cacggtgaag tactcgtaca gcaggctgtg cttgggcagc accttctcgt
    15601 tgggcaggtt cttatcgaag ttggtcatcc gctcgatgaa gctctgggcg ctggcgccct
    15661 tgtccaccac ttcctcgaag ttccaggggg tgatggtttc ctcgctcttt ctggtcatcc
    15721 aggcgaatct gctgtttccc ctggccagag ggcccacgta gtaggggatg cggaaggtca
    15781 ggatcttctc gatcttttcc cggttgtcct tcaggaatgg gtaaaaatct tcctgccgcc
    15841 gcagaatggc gtgcagctct cccaggtgga tctggtgggg gatgctgccg ttgtcgaagg
    15901 tccgctgctt ccgcagcagg tcctctctgt tcagcttcac gagcagttcc tcggtgccgt
    15961 ccatcttttc caggatgggc ttgatgaact tgtagaactc ttcctggctg gctccgccat
    16021 cgatgtagcc ggcgtagccg ttcttgctct ggtcgaagaa aatctctttg tacttctcag
    16081 gcagctgctg ccgcacgaga gctttcagca gggtcaggtc ctggtggtgc tcgtcgtatc
    16141 tcttgatcat agaggcgctc aggggggcct tggtgatctc ggtgttcact ctcaggatgt
    16201 cgctcagcag gatggcgtcg gacaggttct tggcggccag aaacaggtcg gcgtactggt
    16261 cgccgatctg ggccagcagg ttgtccaggt cgtcgtcgta ggtgtccttg ctcagctgca
    16321 gtttggcatc ctcggccagg tcgaagttgc tcttgaagtt gggggtcagg cccaggctca
    16381 gggcaatcag gttgccgaac aggccattct tcttctcgcc gggcagctgg gcgatcagat
    16441 tttccagccg tctgctcttg ctcagtctgg cagacaggat ggccttggcg tccacgccgc
    16501 tggcgttgat ggggttttcc tcgaacagct ggttgtaggt ctgcaccagc tggatgaaca
    16561 gcttgtccac gtcgctgttg tcggggttca ggtcgccctc gatcaggaag tggccccgga
    16621 acttgatcat gtgggccagg gccagataga tcagccgcag gtcggccttg tcggtgctgt
    16681 ccaccagttt ctttctcagg tggtagatgg tggggtactt ctcgtggtag gccacctcgt
    16741 ccacgatgtt gccgaagatg gggtgccgct cgtgcttctt atcctcttcc accaggaagg
    16801 actcttccag tctgtggaag aagctgtcgt ccaccttggc catctcgttg ctgaagatct
    16861 cttgcagata gcagatccgg ttcttccgtc tggtgtatct tcttctggcg gttctcttca
    16921 gccgggtggc ctcggctgtt tctccgctgt cgaacagcag ggcgccgatc aggttcttct
    16981 tgatgctgtg ccggtcggtg ttgcccagca ccttgaattt cttgctgggc accttgtact
    17041 cgtcggtgat cacggcccag cccacagagt tggtgccgat ggccaggccg atgctgtact
    17101 tcttgtccat tacctttctc ttcttttttg gctcagatgg ttcagtgctg gtcccagggg
    17161 cagatccttc acttggttcg gttgacgtgc cttcttcggt ggaagtaggg cttccagctg
    17221 gggaaccagg cgcactacct tcagatggtt ctgtggatgt accagggcct gactcgggcg
    17281 ttgcggattc gctggtgcct tcttcagtag atgttgggga cccggcagga gaccctccac
    17341 ttggtggggg tgcgccagag ctcggccctc cgacccaatg gttatagggc cccgcaacgt
    17401 gtgtgagagc ataaggggac acggtgacaa cattgtcatg ggttaatgtt aatgctttat
    17461 gagaaggaat ttggttcaat tcatttactt cagaggactg ttctggacct tcattagctg
    17521 cctggtcttt ttgctctgag gccttcattt tcttattctt agcttcttta gcctcaaact
    17581 taatcttgtt tagttcaaaa ccatgttggg gcttatttag gtttttgtgc tggtaaaaga
    17641 caagggagag gcgggttgga tgattacggt tggggtgctc aacaggagtg gtagcgtgca
    17701 gctctcgccg ggcacactca atcaaaaccg agccgtgagc aggtgcgatg gccaccccac
    17761 caatatttgc atccaaaaag atgtgctcac tgtctgacca atactcatca atgtggggca
    17821 atttctcctc agcaggtgac agggggtcat cagatagggg ttcgtctgat ggaggctcat
    17881 ctgcttcaga atgctgctca tcttcttcca ttggagaaga ggcaaggtct tgaggagagg
    17941 tgaggaagga gggctggtgg tttggctgat gaggcgttag cggctcagtc acaccagtgg
    18001 aaggctcaga attaatgagg ggctccatca caggagcaga cagggtgggg agaggagcca
    18061 cttcgccaag ctgtgaaatg ccagggccat cagcagctgc agcattggca ccactgagtc
    18121 ttcccgaagg catcgtacag tggggagtgc tgcttctttc tgaaaacccg catgaggctg
    18181 ttgcgtcatt cttcagtgga gctggtgtgg ctgaagcagt cttcggggac caggagaagc
    18241 ctggagatgc ctctttcact gggtgaggag cggatggcat cagcgaataa gttttagtgt
    18301 tgtctgaact ttttaagata aaatggggtt cggtttcact ttttacttca ggttgcacgg
    18361 tttcagtgtt actccctaag gttggcagtg acgaaggctt actgttgttt gttgttgttg
    18421 agttattctt ccgcttgatt cggggaatag gtttcttttc cactgccctt atcttatgtg
    18481 caagaacctc tgtcatcatc gcagccctct tctttccaga acggggaaca ggctgagtga
    18541 aacacgttct ttttttgcgg cggggtgcca ggacctcgat ggccccagat ttgatcttgg
    18601 cttccattcc ttccttggag ccaaactcat ctgtgtctga aagcttataa agaggtagca
    18661 catggagctg ctcatcttga ggaataacac ccaaagagcg gttatcttct cgagttaagg
    18721 tacaaaccac agtgcttcca ttattcatgt tgtgaatgtc cctgtgggga tgagcacaga
    18781 agtccaggca agcagtgacc ccagagaagg gtcgaccttc cttgctgcca agccgacatt
    18841 ctcgggcaac attttcatat tccacctgat tttggtaagc tactggagca tactgcttat
    18901 aaattggagc taatcgtgta gccaaactct gtaagttatc ttcaaggttt ttttcatgta
    18961 agggagagct tggatcaatt ctaaatcttc tggggcttgg gcttctacca aacttacagc
    19021 cattaaagta catactccat gaacagccaa aagagaatga agctccacaa gtctctggat
    19081 caattccttg acatgtacag gtacgatttt cattgagggt gcatcttctg tcggtagggt
    19141 gcccattgta tgactttaga ttctctgtga gctctgtgta tagccggtcg gccattggaa
    19201 gagggatgcc atcccacacc atgatgagca ccaccatcac agcagttgga cagtggtggc
    19261 ctgtacgctg ccggaccaaa caaagaactt tttcttcatc actgcttctt cttaaaaccc
    19321 acttagcaat tggacaccca tgagagcttt tcccttcttt accggtgtac actactattt
    19381 ctatccttat tgcgtttcct ttttgaccat acctattctc catgatttcc ctgacagcag
    19441 caacacttgg tcctgcccca aggtgtgtat aatatgggcc tttgtctttt tgtataactc
    19501 gatcaagaca gctgcaggtg ggcagcatgg ctgtggtgag agaaacagag cgtgagctca
    19561 aatacacaag tacgaaatca gagttatgag agaggatttg gagaaagaag atcacctgaa
    19621 gatcgtttgc gtcgcgcgaa tgagagagac ggcagagaaa acgagaagct ttaagaggac
    19681 gaacaaaaac cctaatgtca ctccttttat atagatgaga aatttagggt ttgggctcta
    19741 atggagctgt ctacatgggc tttcatttct ttatccagta aagaaccggt ttaacagg
//
