LOCUS       sequence-482049-       20140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a CRISPR/dCas9-based fusion protein designed for targeted 
            DNA demethylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20140)
  TITLE     Versatile molecular tools enabling customizable DNA methylation in 
            Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TET1cd-dCas9-p300 (D18)
FEATURES             Location/Qualifiers
     source          1..20140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4103..4123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4124..4150,4151..4153,4154..6640,6641..6643,
                     6644..8227)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4124..8227
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8243..8263
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8267..8287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8336..9031
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             9077..10927
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      10691..10700
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      10966..11218
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(11247..11263)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11247..11263)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11260..11282)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11271..11287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11295..11301,11302..11319,11320..11325))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11340..11361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11552..12228
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12165..12185
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12295..13320
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    13361..13535
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13613..13637
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14062..14856
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14943..15531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15432..15451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15685..15702
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15717..15857
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15783..15802)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15943..15965
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16201..16395
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16461..17534)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17963..18592)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    19893..19917
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     20105..20127
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     20119..20136
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20120..20136
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagac ccggggatat attactcaac ttttgattcg
       61 ctatttgctg tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca
      121 ttgctattac aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca
      181 taaccatttt ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac
      241 cagctccgtt tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc
      301 tttgattgat cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa
      361 acattgcatt cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag
      421 ctttctacat gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa
      481 gcacgaacac cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg
      541 ttgcgatatt gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt
      601 aacaatgaac aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca
      661 ggcttaattt tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag
      721 ataatgcaca agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt
      781 cattaatctc taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa
      841 actacaccaa ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg
      901 gagtgtatat ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata
      961 ttatcggttt atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct
     1021 ttcaatttca tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta
     1081 gcttggagtc aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt
     1141 ttatatcttg caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga
     1201 tcactgactt ggttctaacc aaaaaaaaaa aaaatgttta atttacatat ctaaaagtag
     1261 gtttagggaa acctaaacag taaaatattt gtatattatt cgaatttcac tcatcataaa
     1321 aacttaaatt gcaccataaa attttgtttt actattaatg atgtaatttg tgtaacttaa
     1381 gataaaaata atattccgta agttaaccgg ctaaaaccac gtataaacca gggaacctgt
     1441 taaaccggtt ctttactgga taaagaaatg aaagcccatg tagacagctc cattagagcc
     1501 caaaccctaa atttctcatc tatataaaag gagtgacatt agggtttttg ttcgtcctct
     1561 taaagcttct cgttttctct gccgtctctc tcattcgcgc gacgcaaacg atcttcaggt
     1621 gatcttcttt ctccaaatcc tctctcataa ctctgatttc gtacttgtgt atttgagctc
     1681 acgctctgtt tctctcacca cagccatgct gcccacctgc agctgtcttg atcgagttat
     1741 acaaaaagac aaaggcccat attatacaca ccttggggca ggaccaagtg ttgctgctgt
     1801 cagggaaatc atggagaata ggtatggtca aaaaggaaac gcaataagga tagaaatagt
     1861 agtgtacacc ggtaaagaag ggaaaagctc tcatgggtgt ccaattgcta agtgggtttt
     1921 aagaagaagc agtgatgaag aaaaagttct ttgtttggtc cggcagcgta caggccacca
     1981 ctgtccaact gctgtgatgg tggtgctcat catggtgtgg gatggcatcc ctcttccaat
     2041 ggccgaccgg ctatacacag agctcacaga gaatctaaag tcatacaatg ggcaccctac
     2101 cgacagaaga tgcaccctca atgaaaatcg tacctgtaca tgtcaaggaa ttgatccaga
     2161 gacttgtgga gcttcattct cttttggctg ttcatggagt atgtacttta atggctgtaa
     2221 gtttggtaga agcccaagcc ccagaagatt tagaattgat ccaagctctc ccttacatga
     2281 aaaaaacctt gaagataact tacagagttt ggctacacga ttagctccaa tttataagca
     2341 gtatgctcca gtagcttacc aaaatcaggt ggaatatgaa aatgttgccc gagaatgtcg
     2401 gcttggcagc aaggaaggtc gacccttctc tggggtcact gcttgcctgg acttctgtgc
     2461 tcatccccac agggacattc acaacatgaa taatggaagc actgtggttt gtaccttaac
     2521 tcgagaagat aaccgctctt tgggtgttat tcctcaagat gagcagctcc atgtgctacc
     2581 tctttataag ctttcagaca cagatgagtt tggctccaag gaaggaatgg aagccaagat
     2641 caaatctggg gccatcgagg tcctggcacc ccgccgcaaa aaaagaacgt gtttcactca
     2701 gcctgttccc cgttctggaa agaagagggc tgcgatgatg acagaggttc ttgcacataa
     2761 gataagggca gtggaaaaga aacctattcc ccgaatcaag cggaagaata actcaacaac
     2821 aacaaacaac agtaagcctt cgtcactgcc aaccttaggg agtaacactg aaaccgtgca
     2881 acctgaagta aaaagtgaaa ccgaacccca ttttatctta aaaagttcag acaacactaa
     2941 aacttattcg ctgatgccat ccgctcctca cccagtgaaa gaggcatctc caggcttctc
     3001 ctggtccccg aagactgctt cagccacacc agctccactg aagaatgacg caacagcctc
     3061 atgcgggttt tcagaaagaa gcagcactcc ccactgtacg atgccttcgg gaagactcag
     3121 tggtgccaat gctgcagctg ctgatggccc tggcatttca cagcttggcg aagtggctcc
     3181 tctccccacc ctgtctgctc ctgtgatgga gcccctcatt aattctgagc cttccactgg
     3241 tgtgactgag ccgctaacgc ctcatcagcc aaaccaccag ccctccttcc tcacctctcc
     3301 tcaagacctt gcctcttctc caatggaaga agatgagcag cattctgaag cagatgagcc
     3361 tccatcagac gaacccctat ctgatgaccc cctgtcacct gctgaggaga aattgcccca
     3421 cattgatgag tattggtcag acagtgagca catctttttg gatgcaaata ttggtggggt
     3481 ggccatcgca cctgctcacg gctcggtttt gattgagtgt gcccggcgag agctgcacgc
     3541 taccactcct gttgagcacc ccaaccgtaa tcatccaacc cgcctctccc ttgtctttta
     3601 ccagcacaaa aacctaaata agccccaaca tggttttgaa ctaaacaaga ttaagtttga
     3661 ggctaaagaa gctaagaata agaaaatgaa ggcctcagag caaaaagacc aggcagctaa
     3721 tgaaggtcca gaacagtcct ctgaagtaaa tgaattgaac caaattcctt ctcataaagc
     3781 attaacatta acccatgaca atgttgtcac cgtgtcccct tatgctctca cacacgttgc
     3841 ggggccctat aaccattggg tcggagggcc gagctctggc gcacccccac caagtggagg
     3901 gtctcctgcc gggtccccaa catctactga agaaggcacc agcgaatccg caacgcccga
     3961 gtcaggccct ggtacatcca cagaaccatc tgaaggtagt gcgcctggtt ccccagctgg
     4021 aagccctact tccaccgaag aaggcacgtc aaccgaacca agtgaaggat ctgcccctgg
     4081 gaccagcact gaaccatctg agccaaaaaa gaagagaaag gtaatggaca agaagtacag
     4141 catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
     4201 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
     4261 cctgatcggc gccctgctgt tcgacagcgg agaaacagcc gaggccaccc ggctgaagag
     4321 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
     4381 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
     4441 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
     4501 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
     4561 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
     4621 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
     4681 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
     4741 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
     4801 cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg ccctgagcct
     4861 gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct
     4921 gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta
     4981 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct
     5041 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga
     5101 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa
     5161 gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca tcgatggcgg
     5221 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac
     5281 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga
     5341 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca
     5401 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt
     5461 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac
     5521 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc
     5581 cagcgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa
     5641 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtacaacg agctgaccaa
     5701 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa
     5761 agccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga
     5821 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg
     5881 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
     5941 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
     6001 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
     6061 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
     6121 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
     6181 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
     6241 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
     6301 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
     6361 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
     6421 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
     6481 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
     6541 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
     6601 ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc ctcagagctt
     6661 tctgaaggac gactccatcg ataacaaagt gctgactcgg agcgacaaga accggggcaa
     6721 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcgccagct
     6781 gctgaatgcc aagctgatta cccagaggaa gttcgacaat ctgaccaagg ccgagagagg
     6841 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
     6901 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
     6961 cgacaaactg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
     7021 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
     7081 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
     7141 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
     7201 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
     7261 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
     7321 cggcgaaaca ggcgagatcg tgtgggataa gggccgggac tttgccaccg tgcggaaagt
     7381 gctgtctatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
     7441 caaagagtct atcctgccca agaggaacag cgacaagctg atcgccagaa agaaggactg
     7501 ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt
     7561 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
     7621 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
     7681 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
     7741 ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact
     7801 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
     7861 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca aacactacct
     7921 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa
     7981 tctggacaag gtgctgagcg cctacaacaa gcacagagac aagcctatca gagagcaggc
     8041 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta
     8101 ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac
     8161 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
     8221 aggcgacggt ggcggaggct cgccaaaaaa gaagagaaag gtagacccaa agaaaaaacg
     8281 aaaagtaagc ggaagtgaga ccccaggtac atccgaatca gcaacgcctg aaagcagcga
     8341 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca
     8401 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
     8461 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct
     8521 ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc
     8581 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
     8641 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
     8701 ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt
     8761 caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa
     8821 gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc
     8881 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
     8941 ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga
     9001 cctccctagc aaactggggc acaagcttaa tggcggtggc ggagggatgg atgctaagtc
     9061 actaactgcc tggtccattt tcaaaccaga agaactacga caggcactga tgccaacttt
     9121 ggaggcactt taccgtcagg acccagaatc ccttcccttt cgtcaacctg tggaccctca
     9181 gcttttagga atccctgatt actttgatat tgtgaagagc cccatggatc tttctaccat
     9241 taagaggaag ttagacactg gacagtatca ggagccctgg cagtatgtcg atgatatttg
     9301 gcttatgttc aataatgcct ggttatataa ccggaaaaca tcacgggtat acaaatactg
     9361 ctccaagctc tctgaggtct ttgaacaaga aattgaccca gtgatgcaaa gccttggata
     9421 ctgttgtggc agaaagttgg agttctctcc acagacactg tgttgctacg gcaaacagtt
     9481 gtgcacaata cctcgtgatg ccacttatta cagttaccag aacaggtatc atttctgtga
     9541 gaagtgtttc aatgagatcc aaggggagag cgtttctttg ggggatgacc cttcccagcc
     9601 tcaaactaca ataaataaag aacaattttc caagagaaaa aatgacacac tggaccctga
     9661 actgtttgtt gaatgtacag agtgcggaag aaagatgcat cagatctgtg tccttcacca
     9721 tgagatcatc tggcctgctg gattcgtctg tgatggctgt ttaaagaaaa gtgcacgaac
     9781 taggaaagaa aataagtttt ctgctaaaag gttgccatct accagacttg gcacctttct
     9841 agagaatcgt gtgaatgact ttctgaggcg acagaatcac cctgagtcag gagaggtcac
     9901 tgttagagta gttcatgctt ctgacaaaac cgtggaagta aaaccaggca tgaaagcaag
     9961 gtttgtggac agtggagaga tggcagaatc ctttccatac cgaaccaaag ccctctttgc
    10021 ctttgaagaa attgatggtg ttgacctgtg cttctttggc atgcatgttc aagagtatgg
    10081 ctctgactgc cctccaccca accagaggag agtatacata tcttacctcg atagtgttca
    10141 tttcttccgt cctaaatgct tgaggactgc agtctatcat gaaatcctaa ttggatattt
    10201 agaatatgtc aagaaattag gttacacaac agggcatatt tgggcatgtc caccaagtga
    10261 gggagatgat tatatcttcc attgccatcc tcctgaccag aagataccca agcccaagcg
    10321 actgcaggaa tggtacaaaa aaatgcttga caaggctgta tcagagcgta ttgtccatga
    10381 ctacaaggat atttttaaac aagctactga agatagatta acaagtgcaa aggaattgcc
    10441 ttatttcgag ggtgatttct ggcccaatgt tctggaagaa agcattaagg aactggaaca
    10501 ggaggaagaa gagagaaaac gagaggaaaa caccagcaat gaaagcacag atgtgaccaa
    10561 gggagacagc aaaaatgcta aaaagaagaa taataagaaa accagcaaaa ataagagcag
    10621 cctgagtagg ggcaacaaga agaaaccagg gatgcccaat gtatctaacg acctctcaca
    10681 gaaactatat gccaccatgg agaagcataa agaggtcttc tttgtgatcc gcctcattgc
    10741 tggccctgct gccaactccc tgcctcccat tgttgatcct gatcctctca tcccctgcga
    10801 tctgatggat ggtcgggatg cgtttctcac gctggcaagg gacaagcacc tggagttctc
    10861 ttcactccga agagcccagt ggtccaccat gtgcatgctg gtggagctgc acacgcagag
    10921 ccaggactaa ggatcctgat tgatcgatag agctcgaatt tccccgatcg ttcaaacatt
    10981 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
    11041 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
    11101 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
    11161 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
    11221 ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
    11281 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    11341 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    11401 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    11461 ggctagagca gcttgccaac atggtggagc acgacactct cgtctactcc aagaatatca
    11521 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg
    11581 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
    11641 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
    11701 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
    11761 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgaacatggt ggagcacgac
    11821 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
    11881 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
    11941 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
    12001 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca
    12061 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
    12121 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
    12181 ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct
    12241 ctctctacaa atctatctct ctcgagcttt cgcagatccg gggggcaatg agatatgaaa
    12301 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
    12361 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
    12421 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
    12481 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggag
    12541 tttagcgaga gcctgaccta ttgcatctcc cgccgttcac agggtgtcac gttgcaagac
    12601 ctgcctgaaa ccgaactgcc cgctgttcta caaccggtcg cggaggctat ggatgcgatc
    12661 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
    12721 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
    12781 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgatctgatg
    12841 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
    12901 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
    12961 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
    13021 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccacgactc
    13081 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
    13141 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
    13201 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
    13261 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaagaaatag
    13321 agtagatgcc gaccgggatc tgtcgatcga caagctcgag tttctccata ataatgtgtg
    13381 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
    13441 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
    13501 tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaattcg gcgttaattc
    13561 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
    13621 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
    13681 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
    13741 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
    13801 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
    13861 tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt tatacgccaa
    13921 ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa
    13981 cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag
    14041 aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg
    14101 atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag
    14161 ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc
    14221 acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt
    14281 ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc
    14341 gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag
    14401 ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg
    14461 aatagcttag acagccgctt agccgaattg gattacttac tgaataacga tctggccgat
    14521 gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat
    14581 tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac
    14641 agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc
    14701 agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg
    14761 gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa
    14821 ataaaatatt atattttact ggatgaattg ttttagtacc tagaatgcat gaccaaaatc
    14881 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    14941 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    15001 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    15061 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
    15121 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    15181 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    15241 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    15301 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    15361 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    15421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    15481 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    15541 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    15601 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    15661 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
    15721 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
    15781 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
    15841 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
    15901 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
    15961 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
    16021 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
    16081 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
    16141 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
    16201 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
    16261 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
    16321 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
    16381 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
    16441 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
    16501 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
    16561 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
    16621 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
    16681 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
    16741 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
    16801 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
    16861 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
    16921 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
    16981 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
    17041 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
    17101 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
    17161 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
    17221 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
    17281 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
    17341 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
    17401 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
    17461 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
    17521 ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc
    17581 aggcaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca
    17641 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc
    17701 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc
    17761 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc
    17821 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt
    17881 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc
    17941 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct
    18001 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg
    18061 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc
    18121 cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga
    18181 caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca
    18241 ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg
    18301 ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga
    18361 agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa
    18421 cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg
    18481 aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga
    18541 tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc
    18601 cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct
    18661 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
    18721 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
    18781 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
    18841 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
    18901 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
    18961 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
    19021 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
    19081 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
    19141 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
    19201 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
    19261 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
    19321 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
    19381 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
    19441 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
    19501 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
    19561 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
    19621 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
    19681 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg
    19741 actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt
    19801 ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc
    19861 cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
    19921 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctgctctag
    19981 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
    20041 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
    20101 ttttcccagt cacgacgttg taaaacgacg gccagtgcca
//
