LOCUS       sequence-482057-       16237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses betalain biosynthesis genes under CaMV 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16237)
  AUTHORS   Lee K, Kang M, Ji Q, Grosic S, Wang K
  TITLE     New T-DNA binary vectors with NptII selection and RUBY reporter for 
            efficient maize transformation and targeted mutagenesis.
  JOURNAL   Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231.
  PUBMED    37070560
REFERENCE   2  (bases 1 to 16237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231."
COMMENT     Sequence Label: pCBL101-RUBY
FEATURES             Location/Qualifiers
     source          1..16237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    183..204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(219..224,225..242,243..249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    257..273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     262..284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     281..297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     281..297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        982..1658
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1595..1615
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1667..5617
                     /label=RUBY
     CDS             3167..3223
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4058..4114
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        5993..7988
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    8017..8064
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8084..8878
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8138..8157)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8748..8767
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9445..9462)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9445..9461)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9454..9476)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    9676..9700
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10221..11012
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      11258..11846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11747..11766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12000..12017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12032..12172
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12098..12117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12258..12280
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12516..12710
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12776..13849)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(14278..14907)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
      301 attacgaatt cgagctcgac actgatagtt ttgagacttt tcaacaaagg gtaatatcgg
      361 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
      421 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
      481 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
      541 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgaacatggt ggagcacgac
      601 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
      661 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
      721 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
      781 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca
      841 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
      901 tgatgtgaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      961 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     1021 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
     1081 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
     1141 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
     1201 aaccacgtct tcaaagcaag tggattgatg tgaacatggt ggagcacgac actctcgtct
     1261 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     1321 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
     1381 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
     1441 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1501 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     1561 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
     1621 tataaggaag ttcatttcat ttggagagga cacgctgaaa actagtatgg atcatgcgac
     1681 cctcgccatg atcctcgcga tctggttcat cagcttccac ttcatcaagc tgctgttctc
     1741 ccagcagacc accaagctgc ttccgccagg accaaagccg cttccgatca tcggcaacat
     1801 ccttgaggtg ggcaagaagc cgcatcggtc cttcgccaac ctcgccaaga ttcacggccc
     1861 actcatttcc ctcagactcg gctctgtgac caccatcgtt gtgtcctctg ccgacgtggc
     1921 caaagagatg ttcctcaaga aggatcaccc gctctccaac cgcacgatcc cgaatagtgt
     1981 tacagccggc gaccaccaca agctcaccat gtcttggctc ccggtgtctc cgaagtggcg
     2041 caacttccgc aagattaccg ccgtgcatct gctctcccca cagagactcg atgcctgcca
     2101 gacattcagg cacgccaagg tgcagcagct ctacgagtac gttcaagagt gcgcccagaa
     2161 aggccaggcc gtggatattg gcaaggccgc ctttacgacc agcctcaacc tcctcagcaa
     2221 gctgttcttc agcgtcgagc tggcgcacca caagtcccat accagccaag agttcaaaga
     2281 gctgatctgg aacatcatgg aagatatagg caagccgaac tacgccgact acttcccgat
     2341 tctcggctgc gttgacccat ctggcattag aagaaggctc gcctgctcct tcgacaagct
     2401 gatcgccgtg ttccagggca tcatctgcga gagactcgcc ccagattcct ccaccacaac
     2461 taccaccacc accgacgacg tgctcgatgt gctcctccag ctgttcaagc agaacgagct
     2521 gacgatgggc gagatcaacc acctcctcgt ggacatcttc gacgccggca ccgataccac
     2581 atcctccaca ttcgagtggg tgatgaccga gctgatccgc aatccagaga tgatggaaaa
     2641 ggcccaagag gaaatcaagc aggtcctcgg caaggacaag cagatccaag agtccgacat
     2701 catcaacctg ccgtacctcc aggcgatcat caaagagaca ctccgcctcc atccgccgac
     2761 cgtgttcttg ctcccaagaa aggccgacac cgatgtcgag ctgtacggct acatcgtgcc
     2821 gaaggatgcc cagatcctcg tgaacctctg ggccattggc agggacccaa acgcctggca
     2881 gaacgccgat attttcagcc cagagcgctt catcggctgc gagatcgatg ttaagggccg
     2941 cgatttcggc ctccttccat ttggcgctgg ccgcagaatt tgcccaggca tgaatctcgc
     3001 catcaggatg ctcaccctca tgctcgccac actcctccag ttcttcaact ggaagctcga
     3061 aggcgacatc tccccgaagg acctcgacat ggacgagaag ttcggcattg cgctccaaaa
     3121 gaccaagccg ctcaagctca tcccgattcc gcgctacggt agcggagcta ccaattttag
     3181 cctccttaag caggcaggtg atgtagaaga gaaccccggg cctatgaaga tgatgaacgg
     3241 cgaggacgcc aacgaccaga tgatcaaaga gtccttcttc atcacccacg gcaacccgat
     3301 cctcaccgtc gaggatacac atccgctcag gccgttcttc gagacatggc gcgagaagat
     3361 tttctccaag aagccgaagg ccatcctcat catctccggc cactgggaga cagtgaagcc
     3421 aaccgtgaac gccgtgcaca tcaacgacac catccacgac ttcgacgact acccagccgc
     3481 catgtaccag ttcaagtacc cagctccagg cgagccagag cttgcgagaa aggtggaaga
     3541 gatcctcaag aagtccgggt tcgagacagc cgagacagac caaaagaggg gccttgatca
     3601 cggcgcctgg gttccactca tgctcatgta tccagaggcg gacatcccgg tgtgccagct
     3661 ctcagttcag ccacatctcg acggcaccta ccactacaat ctcggcagag ccctcgcgcc
     3721 gctcaagaat gatggcgtgc tcattattgg ctccggcagc gccacacatc cactcgatga
     3781 gacaccgcac tacttcgatg gtgttgcccc ttgggccgct gccttcgatt cttggcttag
     3841 gaaggccctc atcaacggcc gcttcgagga agtgaacatc tacgagagca aggccccgaa
     3901 ctggaagctc gcccatccat ttccagagca cttctacccg ctccacgttg tgctcggcgc
     3961 tgctggtgaa aagtggaagg ccgagctgat ccactcctcc tgggatcatg gcacactttg
     4021 ccacggctcc tacaagttca cctccgccgg atccggagca accaacttta gcctgctcaa
     4081 gcaagcagga gatgttgagg aaaatcctgg ccccatgacc gccatcaaga tgaacaccaa
     4141 cggcgagggc gagacacagc acatcctcat gatcccgttc atggcgcagg gccacctcag
     4201 gccatttctc gaactcgcca tgttcctcta caagcgctcc cacgtgatca tcaccctgct
     4261 cacaactccg ctcaatgccg gcttcctcag gcacctcctt caccaccatt cctactcctc
     4321 cagcggcatc aggatcgtcg agctgccatt caactccacc aaccacggac tcccaccggg
     4381 catcgagaac accgataagc tcacactccc gctcgtggtg tccctcttcc attccaccat
     4441 cagcctcgat ccgcacctcc gcgattacat ctccaggcat ttcagcccag ccaggccacc
     4501 actctgcgtg atccatgatg tgttcctcgg ctgggttgac caggtggcca aggatgtggg
     4561 ctctacaggc gtggtgttca caacaggcgg cgcttatggc acatccgcct acgtgtccat
     4621 ctggaacgat ctcccgcacc agaactactc cgacgaccaa gagttcccgc tgccaggctt
     4681 cccagagaac cataagttcc gcaggtccca gctccatcgg ttcctcagat atgccgacgg
     4741 ctccgacgat tggtccaagt atttccagcc gcagctccgc cagtccatga agtcttttgg
     4801 ctggctctgc aactccgtgg aagagatcga gacactcggc ttctccatcc tccgcaacta
     4861 caccaagctg ccgatctggg gcatcggccc acttattgct tccccagtgc agcactcctc
     4921 ctccgacaac aattcaacag gcgccgagtt cgtgcagtgg ctcagcctca aagagccgga
     4981 ctccgtcctc tacatctcct tcggctccca gaacacgatc agcccgacgc agatgatgga
     5041 actcgctgct ggccttgagt cctccgagaa gccattcctc tgggtgatca gagccccgtt
     5101 cggcttcgac atcaacgaag agatgcgccc agagtggctg ccagagggct ttgaggaacg
     5161 catgaaggtg aagaaacagg gcaagctcgt gtacaagctc ggcccgcagc ttgagatcct
     5221 caaccatgaa tccatcggcg gctttctcac ccactgcgga tggaacagca tccttgagtc
     5281 tcttcgcgag ggcgttccga tgcttggatg gccacttgct gccgagcagg cctacaacct
     5341 caagtacctc gaagatgaga tgggcgtcgc ggttgagctt gctagaggcc tcgaaggcga
     5401 gatctccaaa gagaaggtca agcgcatcgt cgagatgatc cttgagcgca acgagggctc
     5461 caaaggctgg gagatgaaga atcgcgccgt ggaaatgggc aaaaagctca aggacgccgt
     5521 gaacgaggaa aaagagctga agggctcctc cgtgaaggcg atcgacgatt tcctcgacgc
     5581 cgtcatgcag gccaaacttg agccaagcct ccagtgatag tgaatatgaa gatgaagatg
     5641 aaatatttgg tgtgtcaaat aaaaaggttg tgtgcttaag tttgtgtttt tttcttggct
     5701 tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaacat ctcattataa
     5761 tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc ttctccagca
     5821 tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat aagacctaac
     5881 tgagtaagct agcttgttcg agtattatgg cattgggaaa actgttttcc ggggatcctc
     5941 tagagtcgac ctgcaggcat gcaatagttc cggacttacc ggcgccgcta gcctgcagtg
     6001 cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa
     6061 aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata
     6121 catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg
     6181 ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg
     6241 acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa
     6301 atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg
     6361 ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta
     6421 aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag
     6481 aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg
     6541 aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta
     6601 acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg
     6661 catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc
     6721 cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg
     6781 gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc
     6841 gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa
     6901 cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg
     6961 cacctccgct tcaaggtacg ccgctcgtcc tccccccccc ccctctctac cttctctaga
     7021 tcggcgttcc ggtccatgca tggttagggc ccggtagttc tacttctgtt catgtttgtg
     7081 ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat gcgacctgta
     7141 cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat cctgggatgg
     7201 ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt tgcatagggt
     7261 ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc gggtcatctt
     7321 ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat
     7381 cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt
     7441 gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat
     7501 aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt ttgttcgctt
     7561 ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg gagtagaata
     7621 ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt gtcatacatc
     7681 ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta tacatgttga
     7741 tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa
     7801 ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata
     7861 tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac
     7921 gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac
     7981 ttctgcaggt cgactctaga ggatcaattc gctagcgaag ttcctattcc gaagttccta
     8041 ttctctagaa agtataggaa cttcagatcc accgggatcc accatggttg aacaagatgg
     8101 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     8161 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     8221 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     8281 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     8341 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     8401 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     8461 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     8521 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     8581 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt
     8641 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     8701 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     8761 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     8821 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg
     8881 atccaccatg gttaacctag acttgtccat cttctggatt ggccaactta attaatgtat
     8941 gaaataaaag gatgcacaca tagtgacatg ctaatcacta taatgtgggc atcaaagttg
     9001 tgtgttatgt gtaattacta gttatctgaa taaaagagaa agagatcatc catatttctt
     9061 atcctaaatg aatgtcacgt gtctttataa ttctttgatg aaccagatgc atttcattaa
     9121 ccaaatccat atacatataa atattaatca tatataatta atatcaattg ggttagcaaa
     9181 acaaatctag tctaggtgtg ttttgcaagc ttgcatgcct gcagtcgtct attggaccga
     9241 cagttgctat taatataatg ggccaccata gtagactgac aaataaatta cctgacaaca
     9301 tcgtttcaca aaaaaacaaa cacaaaaagg gagtgcattt tccagggcat ttttgtaata
     9361 aaaaacagtt aaaagggagt gcaatagaaa tataggggtg tggaaatagt gatttgagca
     9421 cgtcttgaag cgaattagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     9481 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     9541 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     9601 ctagagcaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     9661 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     9721 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     9781 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     9841 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     9901 tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga
     9961 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
    10021 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
    10081 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
    10141 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
    10201 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
    10261 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
    10321 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
    10381 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
    10441 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
    10501 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
    10561 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
    10621 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
    10681 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
    10741 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
    10801 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
    10861 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
    10921 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
    10981 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
    11041 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
    11101 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
    11161 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact
    11221 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    11281 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11341 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11401 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11461 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11521 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11581 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11641 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11701 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11761 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11821 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11881 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11941 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    12001 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
    12061 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
    12121 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
    12181 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
    12241 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
    12301 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
    12361 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg
    12421 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
    12481 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
    12541 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
    12601 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
    12661 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac
    12721 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
    12781 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
    12841 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
    12901 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
    12961 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
    13021 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
    13081 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
    13141 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
    13201 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
    13261 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
    13321 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
    13381 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
    13441 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
    13501 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
    13561 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
    13621 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc
    13681 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
    13741 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
    13801 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc
    13861 gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg
    13921 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg
    13981 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca
    14041 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc
    14101 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct
    14161 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg
    14221 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca
    14281 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc
    14341 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg
    14401 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc
    14461 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc
    14521 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta
    14581 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa
    14641 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac
    14701 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac
    14761 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc
    14821 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt
    14881 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat
    14941 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt
    15001 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg
    15061 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc
    15121 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt
    15181 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg
    15241 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct
    15301 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc
    15361 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt
    15421 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg
    15481 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc
    15541 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg
    15601 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc
    15661 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt
    15721 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa
    15781 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt
    15841 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat
    15901 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac
    15961 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt
    16021 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt
    16081 ggatcaaagt actttaaagt actttaaagt actttaaagt actttgatcc cgaggggaac
    16141 cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa
    16201 atatccgatt attctaataa acgctctttt ctcttag
//
