LOCUS       sequence-482097-        7961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of CIBN-eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Krueger D, Spoelstra WK, Mastebroek DJ, Kok RNU, Wu S, Nikolaev M, 
            Bannier-Helaouet M, Gjorevski N, Lutolf M, van Es J, van Zon J, Tans
            SJ, Clevers H
  TITLE     Epithelial tension controls intestinal cell extrusion.
  JOURNAL   Science. 2025 Sep 4;389(6764):eadr8753. doi: 
            10.1126/science.adr8753. Epub 2025 Sep 4.
  PUBMED    40906841
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-09-4"; volume: "389(6764):eadr8753. doi"; pages: " 
            10.1126/science.adr8753. Epub 2025 Sep 4"
COMMENT     Sequence Label: p2T-CAG-CIBN-eGFP-CAAX-Puro
FEATURES             Location/Qualifiers
     source          1..7961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(150..941,942..1010))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     773..792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1011..1115)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1141..1595)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1278..1299)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1490..1509
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1722..1744
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1736..1753
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1737..1753
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1763..1782
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1763..1781
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(1812..1823,1824..1986)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        2026..2405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2687..3703
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3627..3649
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3711..3730
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3775..4284
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     regulatory      4297..4306
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4303..4305,4306..4308,4309..5019)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4303..4305,4306..4308,4309..5019)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4348..4369)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4609..4628)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4956..4977
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5097..5649
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5241..5258)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5468..5487
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5676..6275
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5676..5695)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6172..6192
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6321..6455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6345..6364)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6399..6418
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6625..6645
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(6655..6842,6843..6854)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6911..6931)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6911..6929)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6950..6966)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6950..6966)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6963..6985)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6974..6990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6998..7004,7005..7022,7023..7028))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7043..7064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7181..7198)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7352..7940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7432..7451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
       61 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      121 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      181 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      241 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      301 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      361 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      421 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      481 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      541 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      601 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      661 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      721 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      781 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      841 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      901 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      961 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1021 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1081 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1141 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     1201 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     1261 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     1321 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     1381 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     1441 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     1501 cgaaaggagc gggcgctagg gcgcggcggg tgtggtggtc acgctgcgcg taaccaccac
     1561 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa
     1621 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     1681 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     1741 aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccggcat
     1801 atggttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga aagtacaagt
     1861 acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc ttacttgagt
     1921 aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta aaagtaaaag
     1981 caagaaagat cgatctcgaa ggatctggag gccacaagct tgctcgacat tgattattga
     2041 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     2101 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     2161 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     2221 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     2281 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     2341 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     2401 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     2461 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     2521 gggggggggc cgcgcccggg gggggggggg cggggggggg gggggggggg ggacgaggcg
     2581 gagaggtgcg gcggcagcca ctctgagcgg cgcgctccga aagtttcctt ttatggcgag
     2641 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     2701 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     2761 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     2821 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     2881 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     2941 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     3001 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     3061 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     3121 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     3181 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     3241 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     3301 cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     3361 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     3421 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     3481 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     3541 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     3601 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     3661 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     3721 ggttattgtg ctgtctcatc attttggcaa agaattcctc gagcggccgc caccatgaat
     3781 ggagctatag gaggtgacct tttgctcaat tttcctgaca tgtcggtcct agagcgccaa
     3841 agggctcacc tcaagtacct caatcccacc tttgattctc ctctcgccgg cttctttgcc
     3901 gattcttcaa tgattaccgg cggcgagatg gacagctatc tttcgactgc cggtttgaat
     3961 cttccgatga tgtacggtga gacgacggtg gaaggtgatt caagactctc aatttcgccg
     4021 gaaacgacgc ttgggactgg aaatttcaag gcagcgaagt ttgatacaga gactaaggat
     4081 tgtaatgagg cggcgaagaa gatgacgatg aacagagatg acctagtaga agaaggagaa
     4141 gaagagaagt cgaaaataac agagcaaaac aatgggagca caaaaagcat caagaagatg
     4201 aaacacaaag ccaagaaaga agagaacaat ttctctaatg attcatctaa agtgacgaag
     4261 gaattggaga aaacggatta tattcatgta ccggtcgcca ccatggtgag caagggcgag
     4321 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     4381 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     4441 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     4501 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     4561 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     4621 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     4681 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     4741 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     4801 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     4861 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     4921 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     4981 gccgccggga tcactctcgg catggacgag ctgtataagg gtaaaaagaa gaaaaagaag
     5041 tcaaagacaa agtgtgtaat tatgtagccc gggaattccg cccctctccc cccctaacgt
     5101 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     5161 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     5221 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     5281 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     5341 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     5401 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     5461 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     5521 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     5581 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     5641 atgataatat ggatatggcc acaacggtcg ccaccatgac cgagtacaag cccacggtgc
     5701 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     5761 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     5821 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     5881 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     5941 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     6001 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     6061 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6121 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6181 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6241 gcacctggtg catgacccgc aagcccggtg cctgaggcat ggacgagctg tacaagtagt
     6301 ctagtggatc caccgatgat gatccagaca tgataagata cattgatgag tttggacaaa
     6361 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     6421 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     6481 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     6541 gtggtatggc tgattatgat cctctagatc agatcttcga tggatccaga ccacctcccc
     6601 tgcgagcccg tcgacctcta gcacacccta actgacacac attccacaga attcaagtga
     6661 tctccaaaaa ataagtactt tttgactgta aataaaattg taaggagtaa aaagtacttt
     6721 tttttctaaa aaaatgtaat taagtaaaag taaaagtatt gatttttaat tgtactcaag
     6781 taaagtaaaa atccccaaaa ataatactta agtacagtaa tcaagtaaaa ttactcaagt
     6841 actttacacc tctggttctt gaccccctac cttcagcaag cccagcagat ccgagctcca
     6901 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     6961 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7021 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7081 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7141 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7201 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7261 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7321 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7381 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7441 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7501 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7561 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7621 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7681 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7741 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7801 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7861 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7921 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc t
//
