LOCUS       sequence-48211-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2281
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             97..162
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             169..189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc accatggact acaaagacca tgacggtgat
      121 tataaagatc atgacatcga ttacaaggat gacgatgaca agatggcccc caagaagaag
      181 aggaaggtgg gcattcaccg cggggtacct atggtggact tgaggacact cggttattcg
      241 caacagcaac aggagaaaat caagcctaag gtcaggagca ccgtcgcgca acaccacgag
      301 gcgcttgtgg ggcatggctt cactcatgcg catattgtcg cgctttcaca gcaccctgcg
      361 gcgcttggga cggtggctgt caaataccaa gatatgattg cggccctgcc cgaagccacg
      421 cacgaggcaa ttgtaggggt cggtaaacag tggtcgggag cgcgagcact tgaggcgctg
      481 ctgactgtgg cgggtgagct tagggggcct ccgctccagc tcgacaccgg gcagctgctg
      541 aagatcgcga agagaggggg agtaacagcg gtagaggcag tgcacgcctg gcgcaatgcg
      601 ctcaccgggg cccccttgaa cctgacccca gaccaggtag tcgcaatcgc gaacaataat
      661 gggggaaagc aagccctgga aaccgtgcaa aggttgttgc cggtcctttg tcaagaccac
      721 ggccttacac cggagcaagt cgtggccatt gcaaataata acggtggcaa acaggctctt
      781 gagacggttc agagacttct cccagttctc tgtcaagccc acgggctgac tcccgatcaa
      841 gttgtagcga ttgcgaataa caatggaggg aaacaagcat tggagactgt ccaacggctc
      901 cttcccgtgt tgtgtcaagc cca
//
